Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation in <i>Exopalaemon carinicauda</i>
Eyestalk ablation is an effective method to promote ovarian development in crustaceans. Herein, we performed transcriptome sequencing of ovary and hepatopancreas tissues after eyestalk ablation in <i>Exopalaemon carinicauda</i> to identify genes related to ovarian development. Our analys...
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MDPI AG
2023-04-01
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| author | Shaoting Jia Jitao Li Jianjian Lv Xianyun Ren Jiajia Wang Qiong Wang Ping Liu Jian Li |
| author_facet | Shaoting Jia Jitao Li Jianjian Lv Xianyun Ren Jiajia Wang Qiong Wang Ping Liu Jian Li |
| author_sort | Shaoting Jia |
| collection | DOAJ |
| description | Eyestalk ablation is an effective method to promote ovarian development in crustaceans. Herein, we performed transcriptome sequencing of ovary and hepatopancreas tissues after eyestalk ablation in <i>Exopalaemon carinicauda</i> to identify genes related to ovarian development. Our analyses led to the identification of 97,383 unigenes and 190,757 transcripts, with an average N50 length of 1757 bp. In the ovary, four pathways related to oogenesis and three related to oocyte rapid growth were enriched. In the hepatopancreas, two vitellogenesis-associated transcripts were identified. Furthermore, short time-series expression miner (STEM) and gene ontology (GO) enrichment analyses revealed five terms related to gamete generation. In addition, two-color fluorescent in situ hybridization results suggested that <i>dmrt1</i> might play a vital role in oogenesis during the early stage of ovarian development. Overall, our insights should support future studies focusing on investigating oogenesis and ovarian development in <i>E. carinicauda.</i> |
| first_indexed | 2024-03-11T05:12:57Z |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T05:12:57Z |
| publishDate | 2023-04-01 |
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| record_format | Article |
| series | Biology |
| spelling | doaj.art-51fbe913fafc4cbc83ce6193348a90512023-11-17T18:24:28ZengMDPI AGBiology2079-77372023-04-0112459610.3390/biology12040596Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation in <i>Exopalaemon carinicauda</i>Shaoting Jia0Jitao Li1Jianjian Lv2Xianyun Ren3Jiajia Wang4Qiong Wang5Ping Liu6Jian Li7National Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaNational Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaEyestalk ablation is an effective method to promote ovarian development in crustaceans. Herein, we performed transcriptome sequencing of ovary and hepatopancreas tissues after eyestalk ablation in <i>Exopalaemon carinicauda</i> to identify genes related to ovarian development. Our analyses led to the identification of 97,383 unigenes and 190,757 transcripts, with an average N50 length of 1757 bp. In the ovary, four pathways related to oogenesis and three related to oocyte rapid growth were enriched. In the hepatopancreas, two vitellogenesis-associated transcripts were identified. Furthermore, short time-series expression miner (STEM) and gene ontology (GO) enrichment analyses revealed five terms related to gamete generation. In addition, two-color fluorescent in situ hybridization results suggested that <i>dmrt1</i> might play a vital role in oogenesis during the early stage of ovarian development. Overall, our insights should support future studies focusing on investigating oogenesis and ovarian development in <i>E. carinicauda.</i>https://www.mdpi.com/2079-7737/12/4/596<i>Exopalaemon carinicauda</i>eyestalkovaryhepatopancreasvitellogenesis |
| spellingShingle | Shaoting Jia Jitao Li Jianjian Lv Xianyun Ren Jiajia Wang Qiong Wang Ping Liu Jian Li Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation in <i>Exopalaemon carinicauda</i> Biology <i>Exopalaemon carinicauda</i> eyestalk ovary hepatopancreas vitellogenesis |
| title | Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation in <i>Exopalaemon carinicauda</i> |
| title_full | Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation in <i>Exopalaemon carinicauda</i> |
| title_fullStr | Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation in <i>Exopalaemon carinicauda</i> |
| title_full_unstemmed | Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation in <i>Exopalaemon carinicauda</i> |
| title_short | Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation in <i>Exopalaemon carinicauda</i> |
| title_sort | molecular characterization related to ovary early development mechanisms after eyestalk ablation in i exopalaemon carinicauda i |
| topic | <i>Exopalaemon carinicauda</i> eyestalk ovary hepatopancreas vitellogenesis |
| url | https://www.mdpi.com/2079-7737/12/4/596 |
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