Ontogenic Profile of Hexokinase and Glucokinase mRNA Expressions in Embryonic Chicken Liver and Muscle
The present study describes ontogenic profile of mRNA expressions of hexokinase (HK; EC 2.7.1.1) and glucokinase (GK; EC 2.7.1.2, otherwise known as HKIV) that catalyze the first step of glucose metabolism by cells. The liver and the skeletal muscle were collected from 11 to 21 day embryos (n=6) of...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Japan Poultry Science Association
2013-07-01
|
| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/50/3/50_0120156/_pdf/-char/en |
| _version_ | 1797816177462870016 |
|---|---|
| author | Tushar K. Roy Atsushi Iwasawa Yasutake Shimizu Koji Kageyama Norio Yoshizaki |
| author_facet | Tushar K. Roy Atsushi Iwasawa Yasutake Shimizu Koji Kageyama Norio Yoshizaki |
| author_sort | Tushar K. Roy |
| collection | DOAJ |
| description | The present study describes ontogenic profile of mRNA expressions of hexokinase (HK; EC 2.7.1.1) and glucokinase (GK; EC 2.7.1.2, otherwise known as HKIV) that catalyze the first step of glucose metabolism by cells. The liver and the skeletal muscle were collected from 11 to 21 day embryos (n=6) of the White Leghorn chicken and the mRNAs of HKI and II, two HKs known in the chicken, and GK were measured by semi-quantitative reverse transcription PCR. In the liver, HKI mRNAs gradually decreased during the experimental period, while GK mRNAs gradually increased. In the skeletal muscle, HKI increased on day 13 and was almost stable thereafter, while GK gradually decreased. HKII expression tended to increase on day 13 and remained stable thereafter in both the organs. These results suggest a possibility that the well-established domination of GK to HK in the liver is gradually acquired during embryogenesis. Whether embryonic skeletal muscle expresses active GK protein should be studied further. |
| first_indexed | 2024-03-13T08:33:53Z |
| format | Article |
| id | doaj.art-51fe5a1f84864c7193706659f9903590 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:33:53Z |
| publishDate | 2013-07-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-51fe5a1f84864c7193706659f99035902023-05-31T01:26:12ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862013-07-0150327027410.2141/jpsa.0120156jpsaOntogenic Profile of Hexokinase and Glucokinase mRNA Expressions in Embryonic Chicken Liver and MuscleTushar K. Roy0Atsushi Iwasawa1Yasutake Shimizu2Koji Kageyama3Norio Yoshizaki4United Graduate School of Agricultural Science, Gifu University, JapanFaculty of Applied Biological Sciences, Gifu University, JapanFaculty of Applied Biological Sciences, Gifu University, JapanRiver Basin Research Center, Gifu University, JapanUnited Graduate School of Agricultural Science, Gifu University, JapanThe present study describes ontogenic profile of mRNA expressions of hexokinase (HK; EC 2.7.1.1) and glucokinase (GK; EC 2.7.1.2, otherwise known as HKIV) that catalyze the first step of glucose metabolism by cells. The liver and the skeletal muscle were collected from 11 to 21 day embryos (n=6) of the White Leghorn chicken and the mRNAs of HKI and II, two HKs known in the chicken, and GK were measured by semi-quantitative reverse transcription PCR. In the liver, HKI mRNAs gradually decreased during the experimental period, while GK mRNAs gradually increased. In the skeletal muscle, HKI increased on day 13 and was almost stable thereafter, while GK gradually decreased. HKII expression tended to increase on day 13 and remained stable thereafter in both the organs. These results suggest a possibility that the well-established domination of GK to HK in the liver is gradually acquired during embryogenesis. Whether embryonic skeletal muscle expresses active GK protein should be studied further.https://www.jstage.jst.go.jp/article/jpsa/50/3/50_0120156/_pdf/-char/enchickenembryoglucokinasehexokinaseontogenesis |
| spellingShingle | Tushar K. Roy Atsushi Iwasawa Yasutake Shimizu Koji Kageyama Norio Yoshizaki Ontogenic Profile of Hexokinase and Glucokinase mRNA Expressions in Embryonic Chicken Liver and Muscle The Journal of Poultry Science chicken embryo glucokinase hexokinase ontogenesis |
| title | Ontogenic Profile of Hexokinase and Glucokinase mRNA Expressions in Embryonic Chicken Liver and Muscle |
| title_full | Ontogenic Profile of Hexokinase and Glucokinase mRNA Expressions in Embryonic Chicken Liver and Muscle |
| title_fullStr | Ontogenic Profile of Hexokinase and Glucokinase mRNA Expressions in Embryonic Chicken Liver and Muscle |
| title_full_unstemmed | Ontogenic Profile of Hexokinase and Glucokinase mRNA Expressions in Embryonic Chicken Liver and Muscle |
| title_short | Ontogenic Profile of Hexokinase and Glucokinase mRNA Expressions in Embryonic Chicken Liver and Muscle |
| title_sort | ontogenic profile of hexokinase and glucokinase mrna expressions in embryonic chicken liver and muscle |
| topic | chicken embryo glucokinase hexokinase ontogenesis |
| url | https://www.jstage.jst.go.jp/article/jpsa/50/3/50_0120156/_pdf/-char/en |
| work_keys_str_mv | AT tusharkroy ontogenicprofileofhexokinaseandglucokinasemrnaexpressionsinembryonicchickenliverandmuscle AT atsushiiwasawa ontogenicprofileofhexokinaseandglucokinasemrnaexpressionsinembryonicchickenliverandmuscle AT yasutakeshimizu ontogenicprofileofhexokinaseandglucokinasemrnaexpressionsinembryonicchickenliverandmuscle AT kojikageyama ontogenicprofileofhexokinaseandglucokinasemrnaexpressionsinembryonicchickenliverandmuscle AT norioyoshizaki ontogenicprofileofhexokinaseandglucokinasemrnaexpressionsinembryonicchickenliverandmuscle |