A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate
A carbon nanofibers modified screen-printed carbon sensor (SPCE/CNFs) was applied for the determination of a novel promising anticancer agent candidate (ethyl 8-(4-methoxyphenyl)-4-oxo-4,6,7,8-tetrahydroimidazo[2,1-<i>c</i>][1,2,4]triazine-3-carboxylate, EIMTC) using square-wave voltamme...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/18/5217 |
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| author | Katarzyna Tyszczuk-Rotko Jędrzej Kozak Małgorzata Sztanke Krzysztof Sztanke Ilona Sadok |
| author_facet | Katarzyna Tyszczuk-Rotko Jędrzej Kozak Małgorzata Sztanke Krzysztof Sztanke Ilona Sadok |
| author_sort | Katarzyna Tyszczuk-Rotko |
| collection | DOAJ |
| description | A carbon nanofibers modified screen-printed carbon sensor (SPCE/CNFs) was applied for the determination of a novel promising anticancer agent candidate (ethyl 8-(4-methoxyphenyl)-4-oxo-4,6,7,8-tetrahydroimidazo[2,1-<i>c</i>][1,2,4]triazine-3-carboxylate, EIMTC) using square-wave voltammetry (SWV). It is the first method for the quantitative determination of EIMTC. The modified screen-printed sensor exhibited excellent electrochemical activity in reducing EIMTC. The peak current of EIMTC was found to be linear in two concentration ranges of 2.0 × 10<sup>−9</sup> – 2.0 × 10<sup>−8</sup> mol L<sup>−1</sup> and 2.0 × 10<sup>−8</sup> – 2.0 × 10<sup>−7</sup> mol L<sup>−1</sup>, with a detection limit of 5.0 × 10<sup>−10</sup> mol L<sup>−1</sup>. The connection of flow-cell for the SPCE/CNFs with SWV detection allowed for the successful determination of EIMTC in human serum samples. Ultra-high-performance liquid chromatography coupled to electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-MS/MS) acted as a comparative method in the serum samples analysis. |
| first_indexed | 2024-03-10T16:22:25Z |
| format | Article |
| id | doaj.art-51fe76dc0b3945bcbbdce07520c5bf72 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T16:22:25Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-51fe76dc0b3945bcbbdce07520c5bf722023-11-20T13:34:02ZengMDPI AGSensors1424-82202020-09-012018521710.3390/s20185217A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent CandidateKatarzyna Tyszczuk-Rotko0Jędrzej Kozak1Małgorzata Sztanke2Krzysztof Sztanke3Ilona Sadok4Faculty of Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University in Lublin, 20-031 Lublin, PolandFaculty of Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University in Lublin, 20-031 Lublin, PolandChair and Department of Medical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandLaboratory of Bioorganic Synthesis and Analysis, Chair and Department of Medical Chemistry, Medical University of Lublin, 20-093 Lublin, PolandLaboratory of Separation and Spectroscopic Method Application, Centre for Interdisciplinary Research, Faculty of Science and Health, The John Paul II Catholic University of Lublin, 20-708 Lublin, PolandA carbon nanofibers modified screen-printed carbon sensor (SPCE/CNFs) was applied for the determination of a novel promising anticancer agent candidate (ethyl 8-(4-methoxyphenyl)-4-oxo-4,6,7,8-tetrahydroimidazo[2,1-<i>c</i>][1,2,4]triazine-3-carboxylate, EIMTC) using square-wave voltammetry (SWV). It is the first method for the quantitative determination of EIMTC. The modified screen-printed sensor exhibited excellent electrochemical activity in reducing EIMTC. The peak current of EIMTC was found to be linear in two concentration ranges of 2.0 × 10<sup>−9</sup> – 2.0 × 10<sup>−8</sup> mol L<sup>−1</sup> and 2.0 × 10<sup>−8</sup> – 2.0 × 10<sup>−7</sup> mol L<sup>−1</sup>, with a detection limit of 5.0 × 10<sup>−10</sup> mol L<sup>−1</sup>. The connection of flow-cell for the SPCE/CNFs with SWV detection allowed for the successful determination of EIMTC in human serum samples. Ultra-high-performance liquid chromatography coupled to electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-MS/MS) acted as a comparative method in the serum samples analysis.https://www.mdpi.com/1424-8220/20/18/5217anticancer agent candidatevoltammetric analysisscreen-printed sensorflow systemliquid chromatography |
| spellingShingle | Katarzyna Tyszczuk-Rotko Jędrzej Kozak Małgorzata Sztanke Krzysztof Sztanke Ilona Sadok A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate Sensors anticancer agent candidate voltammetric analysis screen-printed sensor flow system liquid chromatography |
| title | A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate |
| title_full | A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate |
| title_fullStr | A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate |
| title_full_unstemmed | A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate |
| title_short | A Screen-Printed Sensor Coupled with Flow System for Quantitative Determination of a Novel Promising Anticancer Agent Candidate |
| title_sort | screen printed sensor coupled with flow system for quantitative determination of a novel promising anticancer agent candidate |
| topic | anticancer agent candidate voltammetric analysis screen-printed sensor flow system liquid chromatography |
| url | https://www.mdpi.com/1424-8220/20/18/5217 |
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