Clinical significance of CD8‐positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high‐grade serous carcinoma
Abstract Background The clinical significance of CD8‐positive (CD8+) lymphocytes on tumor cell clusters of ascites cell blocks in patients with ovarian high‐grade serous carcinoma (HGSC) was investigated. Methods Among HGSC patients who underwent surgery from January 2014 to December 2019, 38 patien...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.4592 |
| _version_ | 1817987462598754304 |
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| author | Hideki Iwahashi Morikazu Miyamoto Tsubasa Ito Jin Suminokura Taira Hada Hiroki Ishibashi Soichiro Kakimoto Hiroko Matsuura Rie Suzuki Shinya Minabe Susumu Matsukuma Hitoshi Tsuda Masashi Takano |
| author_facet | Hideki Iwahashi Morikazu Miyamoto Tsubasa Ito Jin Suminokura Taira Hada Hiroki Ishibashi Soichiro Kakimoto Hiroko Matsuura Rie Suzuki Shinya Minabe Susumu Matsukuma Hitoshi Tsuda Masashi Takano |
| author_sort | Hideki Iwahashi |
| collection | DOAJ |
| description | Abstract Background The clinical significance of CD8‐positive (CD8+) lymphocytes on tumor cell clusters of ascites cell blocks in patients with ovarian high‐grade serous carcinoma (HGSC) was investigated. Methods Among HGSC patients who underwent surgery from January 2014 to December 2019, 38 patients with ascites cell block were selected. Using these cell blocks and primary ovarian tumor tissue, the presence of CD8+ lymphocytes and the expression of PD‐L1 were examined immunohistochemically. Tumor cell clusters were defined as cell clumps consisting of more than 10 malignant cells in cell block. Cases with at least one CD8+ lymphocyte in tumor cell cluster were defined as positive CD8+ lymphocytes (Group A); others were defined as negative CD8+ lymphocytes (Group B). The tumor tissue CD8+ lymphocytes were counted mechanically. Clinicopathological features were retrospectively compared between the two groups. Results In total, 38 cases were identified: 25 (65.8%) in Group A and 13 (34.2%) in Group B. More cases in Group A were positive for CD4 (p < 0.01), PD‐L1 (p = 0.02), FoxP3 (p = 0.02) and had a higher number of CD8+ lymphocytes in the tissue (p = 0.03). Patients in Group A had better progression‐free survival (p < 0.01) and overall survival (p = 0.04). In multivariate analysis, Group A was an independent prognostic factor for both progression‐free survival (hazard ratio, 0.24; p < 0.01) and overall survival (hazard ratio, 0.21; p = 0.03). Conclusion The presence of CD8+ lymphocytes in tumor cell clusters of ascites was associated with the status of immune reaction in the tissue and prognosis in patients with HGSC and might be useful information of the immune‐associated therapy. |
| first_indexed | 2024-04-14T00:22:44Z |
| format | Article |
| id | doaj.art-52046819e42b45cba87658aeb2afe6e3 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-04-14T00:22:44Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-52046819e42b45cba87658aeb2afe6e32022-12-22T02:22:54ZengWileyCancer Medicine2045-76342022-05-0111102085209510.1002/cam4.4592Clinical significance of CD8‐positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high‐grade serous carcinomaHideki Iwahashi0Morikazu Miyamoto1Tsubasa Ito2Jin Suminokura3Taira Hada4Hiroki Ishibashi5Soichiro Kakimoto6Hiroko Matsuura7Rie Suzuki8Shinya Minabe9Susumu Matsukuma10Hitoshi Tsuda11Masashi Takano12Department of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanDepartment of Laboratory Medicine National Defense Medical College Hospital Tokorozawa JapanDepartment of Laboratory Medicine National Defense Medical College Hospital Tokorozawa JapanDepartment of Basic Pathology National Defense Medical College Hospital Tokorozawa JapanDepartment of Obstetrics and Gynecology National Defense Medical College Hospital Tokorozawa JapanAbstract Background The clinical significance of CD8‐positive (CD8+) lymphocytes on tumor cell clusters of ascites cell blocks in patients with ovarian high‐grade serous carcinoma (HGSC) was investigated. Methods Among HGSC patients who underwent surgery from January 2014 to December 2019, 38 patients with ascites cell block were selected. Using these cell blocks and primary ovarian tumor tissue, the presence of CD8+ lymphocytes and the expression of PD‐L1 were examined immunohistochemically. Tumor cell clusters were defined as cell clumps consisting of more than 10 malignant cells in cell block. Cases with at least one CD8+ lymphocyte in tumor cell cluster were defined as positive CD8+ lymphocytes (Group A); others were defined as negative CD8+ lymphocytes (Group B). The tumor tissue CD8+ lymphocytes were counted mechanically. Clinicopathological features were retrospectively compared between the two groups. Results In total, 38 cases were identified: 25 (65.8%) in Group A and 13 (34.2%) in Group B. More cases in Group A were positive for CD4 (p < 0.01), PD‐L1 (p = 0.02), FoxP3 (p = 0.02) and had a higher number of CD8+ lymphocytes in the tissue (p = 0.03). Patients in Group A had better progression‐free survival (p < 0.01) and overall survival (p = 0.04). In multivariate analysis, Group A was an independent prognostic factor for both progression‐free survival (hazard ratio, 0.24; p < 0.01) and overall survival (hazard ratio, 0.21; p = 0.03). Conclusion The presence of CD8+ lymphocytes in tumor cell clusters of ascites was associated with the status of immune reaction in the tissue and prognosis in patients with HGSC and might be useful information of the immune‐associated therapy.https://doi.org/10.1002/cam4.4592ascites cell blockCD8‐positive lymphocyteshigh‐grade serous carcinomahybrid cell countimmunohistochemical staining |
| spellingShingle | Hideki Iwahashi Morikazu Miyamoto Tsubasa Ito Jin Suminokura Taira Hada Hiroki Ishibashi Soichiro Kakimoto Hiroko Matsuura Rie Suzuki Shinya Minabe Susumu Matsukuma Hitoshi Tsuda Masashi Takano Clinical significance of CD8‐positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high‐grade serous carcinoma Cancer Medicine ascites cell block CD8‐positive lymphocytes high‐grade serous carcinoma hybrid cell count immunohistochemical staining |
| title | Clinical significance of CD8‐positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high‐grade serous carcinoma |
| title_full | Clinical significance of CD8‐positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high‐grade serous carcinoma |
| title_fullStr | Clinical significance of CD8‐positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high‐grade serous carcinoma |
| title_full_unstemmed | Clinical significance of CD8‐positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high‐grade serous carcinoma |
| title_short | Clinical significance of CD8‐positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high‐grade serous carcinoma |
| title_sort | clinical significance of cd8 positive lymphocytes on tumor cell clusters of ascites cell block in ovarian high grade serous carcinoma |
| topic | ascites cell block CD8‐positive lymphocytes high‐grade serous carcinoma hybrid cell count immunohistochemical staining |
| url | https://doi.org/10.1002/cam4.4592 |
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