Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice
Dried spent yeast (DSY) and its hydrolysate (DSYH) were used as low-cost nitrogen supplements to improve ethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP01 under very high gravity (VHG) fermentation (280 g·L−1 of total sugar) conditions. The supplemented DSY and DSYH concen...
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MDPI AG
2013-03-01
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| Series: | Energies |
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| Online Access: | http://www.mdpi.com/1996-1073/6/3/1618 |
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| author | Pattana Laopaiboon Prasit Jaisil Lakkana Laopaiboon Sureerat Suwanapong Naulchan Khongsay |
| author_facet | Pattana Laopaiboon Prasit Jaisil Lakkana Laopaiboon Sureerat Suwanapong Naulchan Khongsay |
| author_sort | Pattana Laopaiboon |
| collection | DOAJ |
| description | Dried spent yeast (DSY) and its hydrolysate (DSYH) were used as low-cost nitrogen supplements to improve ethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP01 under very high gravity (VHG) fermentation (280 g·L−1 of total sugar) conditions. The supplemented DSY and DSYH concentrations were 11, 16 and 21 g·L−1, corresponding to a yeast extract nitrogen content of 6, 9 and 12 g·L−1, respectively. The initial yeast cell concentration for ethanol fermentation was approximately 5 × 107 cells·mL−1. The fermentation was carried out in single batch mode at 30 °C in 1-L air-locked bottles with an agitation rate of 100 rpm. Ethanol production from the juice with and without yeast extract (9 g·L−1) was also performed as control treatments. The results showed that DSY at 21 g·L−1gave the highest ethanol concentration (PE, 107 g·L−1) and yield (Yp/s, 0.47 g·g−1). The use of DSYH at the same DSY concentration improved ethanol productivity (Qp), but not PE and Yp/s. The ethanol production efficiencies of the juice under DSY and DSYH supplementations were markedly higher than those without nutrient supplementation. However, the PE and Qp values of the juice containing 21 g·L−1 of DSY was approximately 7 g·L−1 and 0.62 g·L−1·h−1 lower than those under the presence of yeast extract (9 g·L−1), respectively. At the end of the single batch fermentation under the optimum DSY concentration, the sugar consumption was approximately 80%. Therefore in the repeated-batch fermentation, the initial total sugar was reduced to 240 g·L−1. The results showed that the system could be carried out at least 20 successive batches with the average PE, Yp/s and Qp of 95 g·L−1, 0.46 g·g−1 and 1.45 g·L−1·h−1, respectively. |
| first_indexed | 2024-04-11T20:46:10Z |
| format | Article |
| id | doaj.art-52052f5a1b924da6ab127686a0dc50bb |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-04-11T20:46:10Z |
| publishDate | 2013-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-52052f5a1b924da6ab127686a0dc50bb2022-12-22T04:04:02ZengMDPI AGEnergies1996-10732013-03-01631618163110.3390/en6031618Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum JuicePattana LaopaiboonPrasit JaisilLakkana LaopaiboonSureerat SuwanapongNaulchan KhongsayDried spent yeast (DSY) and its hydrolysate (DSYH) were used as low-cost nitrogen supplements to improve ethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP01 under very high gravity (VHG) fermentation (280 g·L−1 of total sugar) conditions. The supplemented DSY and DSYH concentrations were 11, 16 and 21 g·L−1, corresponding to a yeast extract nitrogen content of 6, 9 and 12 g·L−1, respectively. The initial yeast cell concentration for ethanol fermentation was approximately 5 × 107 cells·mL−1. The fermentation was carried out in single batch mode at 30 °C in 1-L air-locked bottles with an agitation rate of 100 rpm. Ethanol production from the juice with and without yeast extract (9 g·L−1) was also performed as control treatments. The results showed that DSY at 21 g·L−1gave the highest ethanol concentration (PE, 107 g·L−1) and yield (Yp/s, 0.47 g·g−1). The use of DSYH at the same DSY concentration improved ethanol productivity (Qp), but not PE and Yp/s. The ethanol production efficiencies of the juice under DSY and DSYH supplementations were markedly higher than those without nutrient supplementation. However, the PE and Qp values of the juice containing 21 g·L−1 of DSY was approximately 7 g·L−1 and 0.62 g·L−1·h−1 lower than those under the presence of yeast extract (9 g·L−1), respectively. At the end of the single batch fermentation under the optimum DSY concentration, the sugar consumption was approximately 80%. Therefore in the repeated-batch fermentation, the initial total sugar was reduced to 240 g·L−1. The results showed that the system could be carried out at least 20 successive batches with the average PE, Yp/s and Qp of 95 g·L−1, 0.46 g·g−1 and 1.45 g·L−1·h−1, respectively.http://www.mdpi.com/1996-1073/6/3/1618bioethanoldried spent yeastlow cost nitrogenrepeated-batch fermentationsweet sorghum |
| spellingShingle | Pattana Laopaiboon Prasit Jaisil Lakkana Laopaiboon Sureerat Suwanapong Naulchan Khongsay Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice Energies bioethanol dried spent yeast low cost nitrogen repeated-batch fermentation sweet sorghum |
| title | Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice |
| title_full | Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice |
| title_fullStr | Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice |
| title_full_unstemmed | Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice |
| title_short | Dried Spent Yeast and Its Hydrolysate as Nitrogen Supplements for Single Batch and Repeated-Batch Ethanol Fermentation from Sweet Sorghum Juice |
| title_sort | dried spent yeast and its hydrolysate as nitrogen supplements for single batch and repeated batch ethanol fermentation from sweet sorghum juice |
| topic | bioethanol dried spent yeast low cost nitrogen repeated-batch fermentation sweet sorghum |
| url | http://www.mdpi.com/1996-1073/6/3/1618 |
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