Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines
The function of adjuvant in maintaining the long-term immune response to Typhoid conjugate vaccine (TCV) was evaluated in. Two TCV products, Vi-DT and Vi-TT, were formulated in either aluminum phosphate (AlPO4) or aluminum hydroxide (AlOH) as adjuvants and TCV formulated in phosphate buffer saline w...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-06-01
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| Series: | Human Vaccines & Immunotherapeutics |
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| Online Access: | http://dx.doi.org/10.1080/21645515.2018.1431599 |
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| author | Erman Tritama Catur Riani Indra Rudiansyah Arip Hidayat Siti Azizah Kharisnaeni Debbie Sofie Retnoningrum |
| author_facet | Erman Tritama Catur Riani Indra Rudiansyah Arip Hidayat Siti Azizah Kharisnaeni Debbie Sofie Retnoningrum |
| author_sort | Erman Tritama |
| collection | DOAJ |
| description | The function of adjuvant in maintaining the long-term immune response to Typhoid conjugate vaccine (TCV) was evaluated in. Two TCV products, Vi-DT and Vi-TT, were formulated in either aluminum phosphate (AlPO4) or aluminum hydroxide (AlOH) as adjuvants and TCV formulated in phosphate buffer saline were used as controls. In each case, a group of Balb/c mice was injected intramuscularly with two doses of the formulated vaccine at two-week intervals. The anti-Vi IgG responses were monitored by Enzyme-Linked Immunosorbent Assay and the levels of CD4+ T-cells expressing cytokine were characterized using intracellular cytokine staining. All mice immunized by TCV formulated in adjuvant elicited anti-Vi response to a higher level than the group receiving TCV formulated in PBS. The extent of adsorption of TCV in AlOH was greater than that in AlPO4, and this finding correlated well with the observation that the mice immunized with two doses of Vi-DT(AlOH) elicited anti-Vi IgG to a level higher than that seen with Vi-DT(AlPO4). The mice primed with Vi-TT(AlOH) produced lower anti-Vi IgG (25.901 GM) compared to those receiving Vi-TT(AlPO4) (49.219 GM). However, after the second injection, the former raised the antibody level significantly to 137.008 GM while the latter provided a value of only 104.966 GM. The groups of mice vaccinated by TCV formulated in AlOH expressed IL4 at higher levels than the other groups, which correlated positively with the high Anti-Vi IgG in these animals. In conclusion, AlOH could be recommended as an effective adjuvant for TCV to provide a long-term immune response. |
| first_indexed | 2024-03-11T22:45:39Z |
| format | Article |
| id | doaj.art-520538ada5b34d6bbe8d90655fe9f996 |
| institution | Directory Open Access Journal |
| issn | 2164-5515 2164-554X |
| language | English |
| last_indexed | 2024-03-11T22:45:39Z |
| publishDate | 2018-06-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj.art-520538ada5b34d6bbe8d90655fe9f9962023-09-22T08:17:54ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2018-06-011461524152910.1080/21645515.2018.14315991431599Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccinesErman Tritama0Catur Riani1Indra Rudiansyah2Arip Hidayat3Siti Azizah Kharisnaeni4Debbie Sofie Retnoningrum5Laboratory of Pharmaceutical BiotechnologyLaboratory of Pharmaceutical BiotechnologyResearch and Development Division, PT. Bio FarmaResearch and Development Division, PT. Bio FarmaResearch and Development Division, PT. Bio FarmaLaboratory of Pharmaceutical BiotechnologyThe function of adjuvant in maintaining the long-term immune response to Typhoid conjugate vaccine (TCV) was evaluated in. Two TCV products, Vi-DT and Vi-TT, were formulated in either aluminum phosphate (AlPO4) or aluminum hydroxide (AlOH) as adjuvants and TCV formulated in phosphate buffer saline were used as controls. In each case, a group of Balb/c mice was injected intramuscularly with two doses of the formulated vaccine at two-week intervals. The anti-Vi IgG responses were monitored by Enzyme-Linked Immunosorbent Assay and the levels of CD4+ T-cells expressing cytokine were characterized using intracellular cytokine staining. All mice immunized by TCV formulated in adjuvant elicited anti-Vi response to a higher level than the group receiving TCV formulated in PBS. The extent of adsorption of TCV in AlOH was greater than that in AlPO4, and this finding correlated well with the observation that the mice immunized with two doses of Vi-DT(AlOH) elicited anti-Vi IgG to a level higher than that seen with Vi-DT(AlPO4). The mice primed with Vi-TT(AlOH) produced lower anti-Vi IgG (25.901 GM) compared to those receiving Vi-TT(AlPO4) (49.219 GM). However, after the second injection, the former raised the antibody level significantly to 137.008 GM while the latter provided a value of only 104.966 GM. The groups of mice vaccinated by TCV formulated in AlOH expressed IL4 at higher levels than the other groups, which correlated positively with the high Anti-Vi IgG in these animals. In conclusion, AlOH could be recommended as an effective adjuvant for TCV to provide a long-term immune response.http://dx.doi.org/10.1080/21645515.2018.1431599typhoid conjugate vaccineviadjuvanttetanus toxoiddiphteria toxoidanti-vi iggcytokine |
| spellingShingle | Erman Tritama Catur Riani Indra Rudiansyah Arip Hidayat Siti Azizah Kharisnaeni Debbie Sofie Retnoningrum Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines Human Vaccines & Immunotherapeutics typhoid conjugate vaccine vi adjuvant tetanus toxoid diphteria toxoid anti-vi igg cytokine |
| title | Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines |
| title_full | Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines |
| title_fullStr | Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines |
| title_full_unstemmed | Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines |
| title_short | Evaluation of alum-based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines |
| title_sort | evaluation of alum based adjuvant on the immunogenicity of salmonella enterica serovar typhi conjugates vaccines |
| topic | typhoid conjugate vaccine vi adjuvant tetanus toxoid diphteria toxoid anti-vi igg cytokine |
| url | http://dx.doi.org/10.1080/21645515.2018.1431599 |
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