Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation
Abstract Background As an adequate plant protein raw material, soybean meal has attracted extensive attention because of its high protein content, abundant amino acids essential for animals, and many functional nutrients. Feed fermentation is a new way to improve nutrient absorption of animals by in...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-06-01
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| Series: | Chemical and Biological Technologies in Agriculture |
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| Online Access: | https://doi.org/10.1186/s40538-022-00303-5 |
| _version_ | 1828821620633894912 |
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| author | Hao Ma Lei Wang Haojie Yu Weiwei Wang Guofang Wu Guangyong Qin Zhongfang Tan Yanping Wang Huili Pang |
| author_facet | Hao Ma Lei Wang Haojie Yu Weiwei Wang Guofang Wu Guangyong Qin Zhongfang Tan Yanping Wang Huili Pang |
| author_sort | Hao Ma |
| collection | DOAJ |
| description | Abstract Background As an adequate plant protein raw material, soybean meal has attracted extensive attention because of its high protein content, abundant amino acids essential for animals, and many functional nutrients. Feed fermentation is a new way to improve nutrient absorption of animals by inoculating microorganisms, which can also play a probiotic role. In order to screen the lactic acid bacteria with remarkable protease-producing performance and excellent growth and fermentation characteristics, 1000 strains isolated from different materials were evaluated in this study. After primary and re-screening, 22 strains both with protein clear zone diameters larger than 15.00 mm (including the diameter of a hole puncher 10.00 mm) and protease activities greater than 20.00 U/mL were screened out for physiological and biochemical tests, as well as antimicrobial performance tests. Results Strains P (Pig) 15 and ZZUPF (Zhengzhou University Pig Fecal) 95, which have excellent physiological and biochemical characteristics, as well as good inhibition abilities for Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Micrococcus luteus, were selected and used as fermenting agents for 3- and 30-day (d) fermentation of soybean meal. The analysis of microorganisms, fermentation quality, and chemical composition during fermentation revealed that all lactic acid bacteria addition groups had lower harmful bacteria and pH value, as well as higher lactic acid bacteria content and lactic acid level compared with CK and protease-treated groups. These effects were even better especially after 30 days of fermentation. Conclusions This study indicated that the selected strains Lactiplantibacillus plantarum subsp. plantarum P15 and Enterococcus faecalis ZZUPF95 could be considered as potential probiotics for fermenting soybean meal for further research. Graphical abstract |
| first_indexed | 2024-12-12T12:57:39Z |
| format | Article |
| id | doaj.art-520577f765a046b4afe23f8075053fec |
| institution | Directory Open Access Journal |
| issn | 2196-5641 |
| language | English |
| last_indexed | 2024-12-12T12:57:39Z |
| publishDate | 2022-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj.art-520577f765a046b4afe23f8075053fec2022-12-22T00:23:51ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412022-06-019111710.1186/s40538-022-00303-5Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentationHao Ma0Lei Wang1Haojie Yu2Weiwei Wang3Guofang Wu4Guangyong Qin5Zhongfang Tan6Yanping Wang7Huili Pang8Henan Key Lab Ion Beam Bioengineering, School of Physics and Microelectronics, Zhengzhou UniversityAcademy of Animal Science and Veterinary Medicine, Qinghai UniversityHenan Key Lab Ion Beam Bioengineering, School of Physics and Microelectronics, Zhengzhou UniversityHenan Key Lab Ion Beam Bioengineering, School of Physics and Microelectronics, Zhengzhou UniversityAcademy of Animal Science and Veterinary Medicine, Qinghai UniversitySchool of Agricultural Science, Zhengzhou UniversitySchool of Agricultural Science, Zhengzhou UniversitySchool of Agricultural Science, Zhengzhou UniversitySchool of Agricultural Science, Zhengzhou UniversityAbstract Background As an adequate plant protein raw material, soybean meal has attracted extensive attention because of its high protein content, abundant amino acids essential for animals, and many functional nutrients. Feed fermentation is a new way to improve nutrient absorption of animals by inoculating microorganisms, which can also play a probiotic role. In order to screen the lactic acid bacteria with remarkable protease-producing performance and excellent growth and fermentation characteristics, 1000 strains isolated from different materials were evaluated in this study. After primary and re-screening, 22 strains both with protein clear zone diameters larger than 15.00 mm (including the diameter of a hole puncher 10.00 mm) and protease activities greater than 20.00 U/mL were screened out for physiological and biochemical tests, as well as antimicrobial performance tests. Results Strains P (Pig) 15 and ZZUPF (Zhengzhou University Pig Fecal) 95, which have excellent physiological and biochemical characteristics, as well as good inhibition abilities for Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Micrococcus luteus, were selected and used as fermenting agents for 3- and 30-day (d) fermentation of soybean meal. The analysis of microorganisms, fermentation quality, and chemical composition during fermentation revealed that all lactic acid bacteria addition groups had lower harmful bacteria and pH value, as well as higher lactic acid bacteria content and lactic acid level compared with CK and protease-treated groups. These effects were even better especially after 30 days of fermentation. Conclusions This study indicated that the selected strains Lactiplantibacillus plantarum subsp. plantarum P15 and Enterococcus faecalis ZZUPF95 could be considered as potential probiotics for fermenting soybean meal for further research. Graphical abstracthttps://doi.org/10.1186/s40538-022-00303-5Lactic acid bacteriaSoybean mealSolid-state fermentationMicrobial contentFermentation quality |
| spellingShingle | Hao Ma Lei Wang Haojie Yu Weiwei Wang Guofang Wu Guangyong Qin Zhongfang Tan Yanping Wang Huili Pang Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation Chemical and Biological Technologies in Agriculture Lactic acid bacteria Soybean meal Solid-state fermentation Microbial content Fermentation quality |
| title | Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation |
| title_full | Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation |
| title_fullStr | Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation |
| title_full_unstemmed | Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation |
| title_short | Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation |
| title_sort | protease producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation |
| topic | Lactic acid bacteria Soybean meal Solid-state fermentation Microbial content Fermentation quality |
| url | https://doi.org/10.1186/s40538-022-00303-5 |
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