Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy
Diseases of the gastrointestinal tract of horses are caused by many factors and have a complex pathogenesis. Developing effective methods of differential diagnostics is of high fundamental and applied importance. The pathogenesis of diseases of the digestive tract of horses accompanied by the develo...
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MDPI AG
2020-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/7/4/191 |
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| author | Seid-Fatima Borunova Nikolay Tkachev Baylar Iolchiev Zinaida Artyushina Pavel Abramov Marina Nikitina Anastasia Silanteva Neilia Khusnetdinova Vladimir Serezhenkov |
| author_facet | Seid-Fatima Borunova Nikolay Tkachev Baylar Iolchiev Zinaida Artyushina Pavel Abramov Marina Nikitina Anastasia Silanteva Neilia Khusnetdinova Vladimir Serezhenkov |
| author_sort | Seid-Fatima Borunova |
| collection | DOAJ |
| description | Diseases of the gastrointestinal tract of horses are caused by many factors and have a complex pathogenesis. Developing effective methods of differential diagnostics is of high fundamental and applied importance. The pathogenesis of diseases of the digestive tract of horses accompanied by the development of inflammation and oxidative stress, can be associated with a lack of the nitrogen monoxide which controls many signaling pathways in the body. The level of the nitric oxide (NO) is involved in the regulation of the immune and nervous systems, the tone of all the blood vessels, and the courses of many pathological processes. The nitric oxide activates guanylate cyclase (sGC) and leads to vascular relaxation. The aim of this investigation was to study the metabolites of nitric oxide in horses suffered from intestinal diseases. The levels of nitric oxide in the blood serum of horses depending on their age and health state was studied. The concentration of nitrites in the blood serum of horses aged 6–25 years was 3.4 ± 4.2 μM, and in the young horses (1–5 years) the level of this indicator was 8.2 ± 5.4 μM. A sharp decrease in nitrite was observed in all the horses with intestinal diseases of 2 ± 0.9 μM, especially with tympanitic caecun of 0.6 ± 0.4 μM and with spasmodic colic of 1.8 ± 0.5 μM. The level of nitrosylhemoglobin HbNO in the blood of the diseased animals was higher than that in clinically healthy horses, regardless of age. |
| first_indexed | 2024-03-10T14:28:07Z |
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| language | English |
| last_indexed | 2024-03-10T14:28:07Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-5205ad944cb445c7a9c84ba19607e6ca2023-11-20T22:50:08ZengMDPI AGVeterinary Sciences2306-73812020-11-017419110.3390/vetsci7040191Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR SpectroscopySeid-Fatima Borunova0Nikolay Tkachev1Baylar Iolchiev2Zinaida Artyushina3Pavel Abramov4Marina Nikitina5Anastasia Silanteva6Neilia Khusnetdinova7Vladimir Serezhenkov8The Russian State Center for Animal Feed and Drug Standardization and Quality (VGNKI), Zvenigorodskoye Shosse 5, 123022 Moscow, RussiaSemenov Institute of Chemical Physics, Russian Academy of Sciences, 4 Kosygina Street, Building 1, 119991 Moscow, RussiaL.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, RussiaSkryabin, Academy of Veterinary Medicine and Biotechnology, 23, Scryabin Str., 109472 Moscow, RussiaSkryabin, Academy of Veterinary Medicine and Biotechnology, 23, Scryabin Str., 109472 Moscow, RussiaV.M. Gorbatov Federal Research Center for Food Systems of RAS, 26, Talalikhina Str., 109316 Moscow, RussiaL.K. Ernst Federal Science Center for Animal Husbandry, Dubrovitsy 60, Podolsk Municipal District, 142132 Moscow, RussiaSkryabin, Academy of Veterinary Medicine and Biotechnology, 23, Scryabin Str., 109472 Moscow, RussiaSemenov Institute of Chemical Physics, Russian Academy of Sciences, 4 Kosygina Street, Building 1, 119991 Moscow, RussiaDiseases of the gastrointestinal tract of horses are caused by many factors and have a complex pathogenesis. Developing effective methods of differential diagnostics is of high fundamental and applied importance. The pathogenesis of diseases of the digestive tract of horses accompanied by the development of inflammation and oxidative stress, can be associated with a lack of the nitrogen monoxide which controls many signaling pathways in the body. The level of the nitric oxide (NO) is involved in the regulation of the immune and nervous systems, the tone of all the blood vessels, and the courses of many pathological processes. The nitric oxide activates guanylate cyclase (sGC) and leads to vascular relaxation. The aim of this investigation was to study the metabolites of nitric oxide in horses suffered from intestinal diseases. The levels of nitric oxide in the blood serum of horses depending on their age and health state was studied. The concentration of nitrites in the blood serum of horses aged 6–25 years was 3.4 ± 4.2 μM, and in the young horses (1–5 years) the level of this indicator was 8.2 ± 5.4 μM. A sharp decrease in nitrite was observed in all the horses with intestinal diseases of 2 ± 0.9 μM, especially with tympanitic caecun of 0.6 ± 0.4 μM and with spasmodic colic of 1.8 ± 0.5 μM. The level of nitrosylhemoglobin HbNO in the blood of the diseased animals was higher than that in clinically healthy horses, regardless of age.https://www.mdpi.com/2306-7381/7/4/191ddinitrosil iron complexesESR-spectroscopyhorsenitric oxidenitritenitrosyl hemoglobin |
| spellingShingle | Seid-Fatima Borunova Nikolay Tkachev Baylar Iolchiev Zinaida Artyushina Pavel Abramov Marina Nikitina Anastasia Silanteva Neilia Khusnetdinova Vladimir Serezhenkov Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy Veterinary Sciences ddinitrosil iron complexes ESR-spectroscopy horse nitric oxide nitrite nitrosyl hemoglobin |
| title | Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy |
| title_full | Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy |
| title_fullStr | Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy |
| title_full_unstemmed | Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy |
| title_short | Estimation of Nitrite—Nitric Oxide Derivative—In Horses with Intestinal Colic by ESR Spectroscopy |
| title_sort | estimation of nitrite nitric oxide derivative in horses with intestinal colic by esr spectroscopy |
| topic | ddinitrosil iron complexes ESR-spectroscopy horse nitric oxide nitrite nitrosyl hemoglobin |
| url | https://www.mdpi.com/2306-7381/7/4/191 |
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