Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies
<p>Abstract</p> <p>Background</p> <p>Xenotropic Murine Leukemia Virus-related Virus (XMRV) is a human gammaretrovirus recently identified in prostate cancer tissue and in lymphocytes of patients with chronic fatigue syndrome. To establish the etiologic role of XMRV infe...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-08-01
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| Series: | Retrovirology |
| Online Access: | http://www.retrovirology.com/content/7/1/68 |
| _version_ | 1818114507116904448 |
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| author | Qiu Xiaoxing Swanson Priscilla Luk Ka-Cheung Tu Bailin Villinger Francois Das Gupta Jaydip Silverman Robert H Klein Eric A Devare Sushil Schochetman Gerald Hackett John |
| author_facet | Qiu Xiaoxing Swanson Priscilla Luk Ka-Cheung Tu Bailin Villinger Francois Das Gupta Jaydip Silverman Robert H Klein Eric A Devare Sushil Schochetman Gerald Hackett John |
| author_sort | Qiu Xiaoxing |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Xenotropic Murine Leukemia Virus-related Virus (XMRV) is a human gammaretrovirus recently identified in prostate cancer tissue and in lymphocytes of patients with chronic fatigue syndrome. To establish the etiologic role of XMRV infection in human disease requires large scale epidemiologic studies. Development of assays to detect XMRV-specific antibodies would greatly facilitate such studies. However, the nature and kinetics of the antibody response to XMRV infection have yet to be determined.</p> <p>Results</p> <p>Three rhesus macaques were infected with XMRV to determine the dynamics of the antibody responses elicited by infection with XMRV. All macaques developed antibodies to XMRV during the second week of infection, and the predominant responses were to the envelope protein gp70, transmembrane protein p15E, and capsid protein p30. In general, antibody responses to gp70 and p15E appeared early with higher titers than to p30, especially in the early period of seroconversion. Antibodies to gp70, p15E and p30 persisted to 158 days and were substantially boosted by re-infection, thus, were identified as useful serologic markers. Three high-throughput prototype assays were developed using recombinant proteins to detect antibodies to these viral proteins. Both gp70 and p15E prototype assays demonstrated 100% sensitivity by detecting all Western blot (WB) positive serial bleeds from the XMRV-infected macaques and good specificity (99.5-99.9%) with blood donors. Seroconversion sensitivity and specificity of the p30 prototype assay were 92% and 99.4% respectively.</p> <p>Conclusions</p> <p>This study provides the first demonstration of seroconversion patterns elicited by XMRV infection. The nature and kinetics of antibody responses to XMRV in primates were fully characterized. Moreover, key serologic markers useful for detection of XMRV infection were identified. Three prototype immunoassays were developed to detect XMRV-specific antibodies. These assays demonstrated good sensitivity and specificity; thus, they will facilitate large scale epidemiologic studies of XMRV infection in humans.</p> |
| first_indexed | 2024-12-11T03:51:49Z |
| format | Article |
| id | doaj.art-5206d8b51ce840949df09a262a9a65da |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-12-11T03:51:49Z |
| publishDate | 2010-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-5206d8b51ce840949df09a262a9a65da2022-12-22T01:21:52ZengBMCRetrovirology1742-46902010-08-01716810.1186/1742-4690-7-68Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studiesQiu XiaoxingSwanson PriscillaLuk Ka-CheungTu BailinVillinger FrancoisDas Gupta JaydipSilverman Robert HKlein Eric ADevare SushilSchochetman GeraldHackett John<p>Abstract</p> <p>Background</p> <p>Xenotropic Murine Leukemia Virus-related Virus (XMRV) is a human gammaretrovirus recently identified in prostate cancer tissue and in lymphocytes of patients with chronic fatigue syndrome. To establish the etiologic role of XMRV infection in human disease requires large scale epidemiologic studies. Development of assays to detect XMRV-specific antibodies would greatly facilitate such studies. However, the nature and kinetics of the antibody response to XMRV infection have yet to be determined.</p> <p>Results</p> <p>Three rhesus macaques were infected with XMRV to determine the dynamics of the antibody responses elicited by infection with XMRV. All macaques developed antibodies to XMRV during the second week of infection, and the predominant responses were to the envelope protein gp70, transmembrane protein p15E, and capsid protein p30. In general, antibody responses to gp70 and p15E appeared early with higher titers than to p30, especially in the early period of seroconversion. Antibodies to gp70, p15E and p30 persisted to 158 days and were substantially boosted by re-infection, thus, were identified as useful serologic markers. Three high-throughput prototype assays were developed using recombinant proteins to detect antibodies to these viral proteins. Both gp70 and p15E prototype assays demonstrated 100% sensitivity by detecting all Western blot (WB) positive serial bleeds from the XMRV-infected macaques and good specificity (99.5-99.9%) with blood donors. Seroconversion sensitivity and specificity of the p30 prototype assay were 92% and 99.4% respectively.</p> <p>Conclusions</p> <p>This study provides the first demonstration of seroconversion patterns elicited by XMRV infection. The nature and kinetics of antibody responses to XMRV in primates were fully characterized. Moreover, key serologic markers useful for detection of XMRV infection were identified. Three prototype immunoassays were developed to detect XMRV-specific antibodies. These assays demonstrated good sensitivity and specificity; thus, they will facilitate large scale epidemiologic studies of XMRV infection in humans.</p>http://www.retrovirology.com/content/7/1/68 |
| spellingShingle | Qiu Xiaoxing Swanson Priscilla Luk Ka-Cheung Tu Bailin Villinger Francois Das Gupta Jaydip Silverman Robert H Klein Eric A Devare Sushil Schochetman Gerald Hackett John Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies Retrovirology |
| title | Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies |
| title_full | Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies |
| title_fullStr | Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies |
| title_full_unstemmed | Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies |
| title_short | Characterization of antibodies elicited by XMRV infection and development of immunoassays useful for epidemiologic studies |
| title_sort | characterization of antibodies elicited by xmrv infection and development of immunoassays useful for epidemiologic studies |
| url | http://www.retrovirology.com/content/7/1/68 |
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