Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticles
ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe3O4 nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-06-01
|
| Series: | Biotechnology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X1630011X |
| _version_ | 1818562390393880576 |
|---|---|
| author | Honghua Jia Fan Huang Zhen Gao Chao Zhong Hua Zhou Min Jiang Ping Wei |
| author_facet | Honghua Jia Fan Huang Zhen Gao Chao Zhong Hua Zhou Min Jiang Ping Wei |
| author_sort | Honghua Jia |
| collection | DOAJ |
| description | ω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe3O4 nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe3O4 nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe3O4 nanoparticles was located between 30–40 nm ω-TA was immobilized onto magnetic PVA-Fe3O4 nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6–8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes. |
| first_indexed | 2024-12-14T01:03:07Z |
| format | Article |
| id | doaj.art-52070e0ad8fe4f1cad8c972166e821ba |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-12-14T01:03:07Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-52070e0ad8fe4f1cad8c972166e821ba2022-12-21T23:23:06ZengElsevierBiotechnology Reports2215-017X2016-06-0110C495510.1016/j.btre.2016.03.004Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticlesHonghua JiaFan HuangZhen GaoChao ZhongHua ZhouMin JiangPing Weiω-Transaminase (ω-TA) as a kind of important biocatalyst is widely used in preparation of chiral intermediates. In this paper, a magnetic PVA-Fe3O4 nanoparticles was prepared and employed on immobilization of ω-TA to reduce the cost, increase reusability and enhance stability. The prepared magnetic PVA-Fe3O4 nanoparticles were characterized by transmission electron microscope (TEM), X-ray diffraction (XRD) and vibrating sample magnetometer (VSM). The average size of magnetic PVA-Fe3O4 nanoparticles was located between 30–40 nm ω-TA was immobilized onto magnetic PVA-Fe3O4 nanoparticles via glutaraldehyde cross-linking, achieving a wider pH range as 6–8 and also a better thermal stability at 60 °C. Meanwhile, the immobilized ω-TA could be successfully reused for 13 times in biotransformation. These results therefore indicated that the immobilized ω-TA with high stability might be readily utilized in industrial purposes.http://www.sciencedirect.com/science/article/pii/S2215017X1630011Xω-transaminaseMagnetic PVA-Fe3O4 nanoparticlesImmobilizationChiral amine |
| spellingShingle | Honghua Jia Fan Huang Zhen Gao Chao Zhong Hua Zhou Min Jiang Ping Wei Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticles Biotechnology Reports ω-transaminase Magnetic PVA-Fe3O4 nanoparticles Immobilization Chiral amine |
| title | Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticles |
| title_full | Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticles |
| title_fullStr | Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticles |
| title_full_unstemmed | Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticles |
| title_short | Immobilization of ω-transaminase by magnetic PVA-Fe3O4 nanoparticles |
| title_sort | immobilization of ω transaminase by magnetic pva fe3o4 nanoparticles |
| topic | ω-transaminase Magnetic PVA-Fe3O4 nanoparticles Immobilization Chiral amine |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X1630011X |
| work_keys_str_mv | AT honghuajia immobilizationofōtransaminasebymagneticpvafe3o4nanoparticles AT fanhuang immobilizationofōtransaminasebymagneticpvafe3o4nanoparticles AT zhengao immobilizationofōtransaminasebymagneticpvafe3o4nanoparticles AT chaozhong immobilizationofōtransaminasebymagneticpvafe3o4nanoparticles AT huazhou immobilizationofōtransaminasebymagneticpvafe3o4nanoparticles AT minjiang immobilizationofōtransaminasebymagneticpvafe3o4nanoparticles AT pingwei immobilizationofōtransaminasebymagneticpvafe3o4nanoparticles |