A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus
Abstract Haemonchus contortus is a gastrointestinal nematode that is responsible for high mortality rates in ruminant herds. The resistance of nematodes to synthetic anthelmintics is widespread and requires a continuous search for new bioactive molecules, such as proteins. The objective of this stud...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2018-11-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400473&lng=en&tlng=en |
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| author | Ledia Feitosa Wanderley Alexandra Martins dos Santos Soares Carolina Rocha e Silva Isaias Moreira de Figueiredo Andre Teixeira da Silva Ferreira Jonas Perales Handerson Ribeiro de Oliveira Mota Jose Tadeu Abreu Oliveira Livio Martins Costa Junior |
| author_facet | Ledia Feitosa Wanderley Alexandra Martins dos Santos Soares Carolina Rocha e Silva Isaias Moreira de Figueiredo Andre Teixeira da Silva Ferreira Jonas Perales Handerson Ribeiro de Oliveira Mota Jose Tadeu Abreu Oliveira Livio Martins Costa Junior |
| author_sort | Ledia Feitosa Wanderley |
| collection | DOAJ |
| description | Abstract Haemonchus contortus is a gastrointestinal nematode that is responsible for high mortality rates in ruminant herds. The resistance of nematodes to synthetic anthelmintics is widespread and requires a continuous search for new bioactive molecules, such as proteins. The objective of this study was to evaluate the anthelmintic potential of a protease purified from the latex of Ficus benjamina against H. contortus . Fresh latex was collected from plants via small incisions in the green stems, the rubber was removed by centrifugation, and the latex protein extract (LPE) was obtained. After LPE fractionation with ammonium sulfate and chromatography of the fraction containing the highest proteolytic activity on CM-cellulose, a cysteine protease (FbP) was purified. FbP has a molecular mass of approximately 23.97 kDa, and its proteolytic activity was stable between pH 6.0 and pH 10 and over a broad temperature range, with optimum activity at 60 °C. FbP inhibited both the development and exsheathment of H. contortus larvae, with 50% effective concentrations of 0.26 and 0.79 mg/mL, respectively. We conclude that this cysteine protease from F. benjamina latex with anthelmintic activity against H. contortus could be a promising alternative for the development of products for use in parasite control programmes. |
| first_indexed | 2024-04-12T16:28:14Z |
| format | Article |
| id | doaj.art-5208cc47aa2a4ca393482ed41e7d08c9 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-04-12T16:28:14Z |
| publishDate | 2018-11-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-5208cc47aa2a4ca393482ed41e7d08c92022-12-22T03:25:15ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612018-11-0127447348010.1590/s1984-296120180070S1984-29612018000400473A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortusLedia Feitosa WanderleyAlexandra Martins dos Santos SoaresCarolina Rocha e SilvaIsaias Moreira de FigueiredoAndre Teixeira da Silva FerreiraJonas PeralesHanderson Ribeiro de Oliveira MotaJose Tadeu Abreu OliveiraLivio Martins Costa JuniorAbstract Haemonchus contortus is a gastrointestinal nematode that is responsible for high mortality rates in ruminant herds. The resistance of nematodes to synthetic anthelmintics is widespread and requires a continuous search for new bioactive molecules, such as proteins. The objective of this study was to evaluate the anthelmintic potential of a protease purified from the latex of Ficus benjamina against H. contortus . Fresh latex was collected from plants via small incisions in the green stems, the rubber was removed by centrifugation, and the latex protein extract (LPE) was obtained. After LPE fractionation with ammonium sulfate and chromatography of the fraction containing the highest proteolytic activity on CM-cellulose, a cysteine protease (FbP) was purified. FbP has a molecular mass of approximately 23.97 kDa, and its proteolytic activity was stable between pH 6.0 and pH 10 and over a broad temperature range, with optimum activity at 60 °C. FbP inhibited both the development and exsheathment of H. contortus larvae, with 50% effective concentrations of 0.26 and 0.79 mg/mL, respectively. We conclude that this cysteine protease from F. benjamina latex with anthelmintic activity against H. contortus could be a promising alternative for the development of products for use in parasite control programmes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400473&lng=en&tlng=enProteasenematodecysteine proteaseparasite controlsmall ruminant |
| spellingShingle | Ledia Feitosa Wanderley Alexandra Martins dos Santos Soares Carolina Rocha e Silva Isaias Moreira de Figueiredo Andre Teixeira da Silva Ferreira Jonas Perales Handerson Ribeiro de Oliveira Mota Jose Tadeu Abreu Oliveira Livio Martins Costa Junior A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus Revista Brasileira de Parasitologia Veterinária Protease nematode cysteine protease parasite control small ruminant |
| title | A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus |
| title_full | A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus |
| title_fullStr | A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus |
| title_full_unstemmed | A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus |
| title_short | A cysteine protease from the latex of Ficus benjamina has in vitro anthelmintic activity against Haemonchus contortus |
| title_sort | cysteine protease from the latex of ficus benjamina has in vitro anthelmintic activity against haemonchus contortus |
| topic | Protease nematode cysteine protease parasite control small ruminant |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018000400473&lng=en&tlng=en |
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