Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera)
Abstract Objectives DNA Barcoding has proven to be a reliable method for rapid insect identification. The success of this method is based on the amplification of a specific region, the ‘Folmer’ barcode region at the 5´ start of the cytochrome c oxidase 1 gene (cox1), with universal primers. Previous...
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-023-06585-8 |
| _version_ | 1797630447171141632 |
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| author | Saskia Bastin Diana M. Percy Felipe Siverio |
| author_facet | Saskia Bastin Diana M. Percy Felipe Siverio |
| author_sort | Saskia Bastin |
| collection | DOAJ |
| description | Abstract Objectives DNA Barcoding has proven to be a reliable method for rapid insect identification. The success of this method is based on the amplification of a specific region, the ‘Folmer’ barcode region at the 5´ start of the cytochrome c oxidase 1 gene (cox1), with universal primers. Previous studies showed failures of standard “universal” primers to amplify this region in psyllids. The aim of the study was the design of a new alternative more reliable primer combination for taxa of the superfamily Psylloidea and its comparison with the performance of the standard “universal” Folmer-primers. Results A newly designed degenerate forward primer LCOP-F was developed following comparison of the sequence alignment of the priming site of “universal” primer LCO1490 and the standard insect forward primer LepF1. When combined with the “universal” reverse primer, HCO2198, this new primer pairing was able to generate barcode sequence for all 36 species in 20 genera across the five families of psyllids tested in this study, and these primers were found to be more universally reliable across psyllid taxa than other primer pairs tested. |
| first_indexed | 2024-03-11T11:08:22Z |
| format | Article |
| id | doaj.art-520bcff54e0441269992cb875bf89999 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-03-11T11:08:22Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-520bcff54e0441269992cb875bf899992023-11-12T12:05:32ZengBMCBMC Research Notes1756-05002023-11-011611710.1186/s13104-023-06585-8Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera)Saskia Bastin0Diana M. Percy1Felipe Siverio2Instituto Canario de Investigaciones Agrarias, Unidad de Protección VegetalBotany Department and Biodiversity Research Centre, University of British ColumbiaInstituto Canario de Investigaciones Agrarias, Unidad de Protección VegetalAbstract Objectives DNA Barcoding has proven to be a reliable method for rapid insect identification. The success of this method is based on the amplification of a specific region, the ‘Folmer’ barcode region at the 5´ start of the cytochrome c oxidase 1 gene (cox1), with universal primers. Previous studies showed failures of standard “universal” primers to amplify this region in psyllids. The aim of the study was the design of a new alternative more reliable primer combination for taxa of the superfamily Psylloidea and its comparison with the performance of the standard “universal” Folmer-primers. Results A newly designed degenerate forward primer LCOP-F was developed following comparison of the sequence alignment of the priming site of “universal” primer LCO1490 and the standard insect forward primer LepF1. When combined with the “universal” reverse primer, HCO2198, this new primer pairing was able to generate barcode sequence for all 36 species in 20 genera across the five families of psyllids tested in this study, and these primers were found to be more universally reliable across psyllid taxa than other primer pairs tested.https://doi.org/10.1186/s13104-023-06585-8PsyllidsMolecular identificationPrimer efficacySpecies identificationCOI barcodeStandard barcode |
| spellingShingle | Saskia Bastin Diana M. Percy Felipe Siverio Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera) BMC Research Notes Psyllids Molecular identification Primer efficacy Species identification COI barcode Standard barcode |
| title | Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera) |
| title_full | Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera) |
| title_fullStr | Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera) |
| title_full_unstemmed | Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera) |
| title_short | Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera) |
| title_sort | establishing reliable dna barcoding primers for jumping plant lice psylloidea hemiptera |
| topic | Psyllids Molecular identification Primer efficacy Species identification COI barcode Standard barcode |
| url | https://doi.org/10.1186/s13104-023-06585-8 |
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