Mapping the Function of Whole‐Brain Projection at the Single Neuron Level
Abstract Axonal projection conveys neural information. The divergent and diverse projections of individual neurons imply the complexity of information flow. It is necessary to investigate the relationship between the projection and functional information at the single neuron level for understanding...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-11-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202202553 |
| _version_ | 1798015669962276864 |
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| author | Wei Zhou Shanshan Ke Wenwei Li Jing Yuan Xiangning Li Rui Jin Xueyan Jia Tao Jiang Zimin Dai Guannan He Zhiwei Fang Liang Shi Qi Zhang Hui Gong Qingming Luo Wenzhi Sun Anan Li Pengcheng Li |
| author_facet | Wei Zhou Shanshan Ke Wenwei Li Jing Yuan Xiangning Li Rui Jin Xueyan Jia Tao Jiang Zimin Dai Guannan He Zhiwei Fang Liang Shi Qi Zhang Hui Gong Qingming Luo Wenzhi Sun Anan Li Pengcheng Li |
| author_sort | Wei Zhou |
| collection | DOAJ |
| description | Abstract Axonal projection conveys neural information. The divergent and diverse projections of individual neurons imply the complexity of information flow. It is necessary to investigate the relationship between the projection and functional information at the single neuron level for understanding the rules of neural circuit assembly, but a gap remains due to a lack of methods to map the function to whole‐brain projection. Here an approach is developed to bridge two‐photon calcium imaging in vivo with high‐resolution whole‐brain imaging based on sparse labeling with the genetically encoded calcium indicator GCaMP6. Reliable whole‐brain projections are captured by the high‐definition fluorescent micro‐optical sectioning tomography (HD‐fMOST). A cross‐modality cell matching is performed and the functional annotation of whole‐brain projection at the single‐neuron level (FAWPS) is obtained. Applying it to the layer 2/3 (L2/3) neurons in mouse visual cortex, the relationship is investigated between functional preferences and axonal projection features. The functional preference of projection motifs and the correlation between axonal length in MOs and neuronal orientation selectivity, suggest that projection motif‐defined neurons form a functionally specific information flow, and the projection strength in specific targets relates to the information clarity. This pipeline provides a new way to understand the principle of neuronal information transmission. |
| first_indexed | 2024-04-11T15:38:06Z |
| format | Article |
| id | doaj.art-520e99eb04554f3fb7f8c989040e6383 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-04-11T15:38:06Z |
| publishDate | 2022-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-520e99eb04554f3fb7f8c989040e63832022-12-22T04:15:54ZengWileyAdvanced Science2198-38442022-11-01933n/an/a10.1002/advs.202202553Mapping the Function of Whole‐Brain Projection at the Single Neuron LevelWei Zhou0Shanshan Ke1Wenwei Li2Jing Yuan3Xiangning Li4Rui Jin5Xueyan Jia6Tao Jiang7Zimin Dai8Guannan He9Zhiwei Fang10Liang Shi11Qi Zhang12Hui Gong13Qingming Luo14Wenzhi Sun15Anan Li16Pengcheng Li17Britton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaResearch Unit of Multimodal Cross Scale Neural Signal Detection and Imaging Chinese Academy of Medical Sciences HUST‐Suzhou Institute for Brainsmatics JITRI Suzhou 215100 ChinaResearch Unit of Multimodal Cross Scale Neural Signal Detection and Imaging Chinese Academy of Medical Sciences HUST‐Suzhou Institute for Brainsmatics JITRI Suzhou 215100 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaKey Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering Hainan University Haikou 570228 ChinaChinese Institute for Brain Research Beijing 102206 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaBritton Chance Center and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 ChinaAbstract Axonal projection conveys neural information. The divergent and diverse projections of individual neurons imply the complexity of information flow. It is necessary to investigate the relationship between the projection and functional information at the single neuron level for understanding the rules of neural circuit assembly, but a gap remains due to a lack of methods to map the function to whole‐brain projection. Here an approach is developed to bridge two‐photon calcium imaging in vivo with high‐resolution whole‐brain imaging based on sparse labeling with the genetically encoded calcium indicator GCaMP6. Reliable whole‐brain projections are captured by the high‐definition fluorescent micro‐optical sectioning tomography (HD‐fMOST). A cross‐modality cell matching is performed and the functional annotation of whole‐brain projection at the single‐neuron level (FAWPS) is obtained. Applying it to the layer 2/3 (L2/3) neurons in mouse visual cortex, the relationship is investigated between functional preferences and axonal projection features. The functional preference of projection motifs and the correlation between axonal length in MOs and neuronal orientation selectivity, suggest that projection motif‐defined neurons form a functionally specific information flow, and the projection strength in specific targets relates to the information clarity. This pipeline provides a new way to understand the principle of neuronal information transmission.https://doi.org/10.1002/advs.202202553whole‐brain projectiontwo‐photon calcium imaging in vivohigh‐definition fluorescent micro‐optical sectioning tomography (HD‐fMOST)GCaMP6primary visual cortex |
| spellingShingle | Wei Zhou Shanshan Ke Wenwei Li Jing Yuan Xiangning Li Rui Jin Xueyan Jia Tao Jiang Zimin Dai Guannan He Zhiwei Fang Liang Shi Qi Zhang Hui Gong Qingming Luo Wenzhi Sun Anan Li Pengcheng Li Mapping the Function of Whole‐Brain Projection at the Single Neuron Level Advanced Science whole‐brain projection two‐photon calcium imaging in vivo high‐definition fluorescent micro‐optical sectioning tomography (HD‐fMOST) GCaMP6 primary visual cortex |
| title | Mapping the Function of Whole‐Brain Projection at the Single Neuron Level |
| title_full | Mapping the Function of Whole‐Brain Projection at the Single Neuron Level |
| title_fullStr | Mapping the Function of Whole‐Brain Projection at the Single Neuron Level |
| title_full_unstemmed | Mapping the Function of Whole‐Brain Projection at the Single Neuron Level |
| title_short | Mapping the Function of Whole‐Brain Projection at the Single Neuron Level |
| title_sort | mapping the function of whole brain projection at the single neuron level |
| topic | whole‐brain projection two‐photon calcium imaging in vivo high‐definition fluorescent micro‐optical sectioning tomography (HD‐fMOST) GCaMP6 primary visual cortex |
| url | https://doi.org/10.1002/advs.202202553 |
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