Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing <i>Azospirillum</i> PGPR
Crop varieties differ in their ability to interact with Plant Growth-Promoting Rhizobacteria (PGPR), but the genetic basis for these differences is unknown. This issue was addressed with the PGPR <i>Azospirillum baldaniorum</i> Sp245, using 187 wheat accessions. We screened the accession...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1615 |
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| author | Jordan Valente Florence Gerin Agathe Mini Rohan Richard Jacques Le Gouis Claire Prigent-Combaret Yvan Moënne-Loccoz |
| author_facet | Jordan Valente Florence Gerin Agathe Mini Rohan Richard Jacques Le Gouis Claire Prigent-Combaret Yvan Moënne-Loccoz |
| author_sort | Jordan Valente |
| collection | DOAJ |
| description | Crop varieties differ in their ability to interact with Plant Growth-Promoting Rhizobacteria (PGPR), but the genetic basis for these differences is unknown. This issue was addressed with the PGPR <i>Azospirillum baldaniorum</i> Sp245, using 187 wheat accessions. We screened the accessions based on the seedling colonization by the PGPR and the expression of the phenylpyruvate decarboxylase gene <i>ppdC</i> (for synthesis of the auxin indole-3-acetic acid), using <i>gusA</i> fusions. Then, the effects of the PGPR on the selected accessions stimulating Sp245 (or not) were compared in soil under stress. Finally, a genome-wide association approach was implemented to identify the quantitative trait loci (QTL) associated with PGPR interaction. Overall, the ancient genotypes were more effective than the modern genotypes for <i>Azospirillum</i> root colonization and <i>ppdC</i> expression. In non-sterile soil, <i>A. baldaniorum</i> Sp245 improved wheat performance for three of the four PGPR-stimulating genotypes and none of the four non-PGPR-stimulating genotypes. The genome-wide association did not identify any region for root colonization but revealed 22 regions spread on 11 wheat chromosomes for <i>ppdC</i> expression and/or <i>ppdC</i> induction rate. This is the first QTL study focusing on molecular interaction with PGPR bacteria. The molecular markers identified provide the possibility to improve the capacity of modern wheat genotypes to interact with Sp245, as well as, potentially, other <i>Azospirillum</i> strains. |
| first_indexed | 2024-03-11T02:08:11Z |
| format | Article |
| id | doaj.art-5215261ecdbb41feaaa3924fcb4d5bd3 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:11Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5215261ecdbb41feaaa3924fcb4d5bd32023-11-18T11:44:20ZengMDPI AGMicroorganisms2076-26072023-06-01116161510.3390/microorganisms11061615Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing <i>Azospirillum</i> PGPRJordan Valente0Florence Gerin1Agathe Mini2Rohan Richard3Jacques Le Gouis4Claire Prigent-Combaret5Yvan Moënne-Loccoz6Univ Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR5557 Ecologie Microbienne, 43 Bd du 11 Novembre 1918, F-69622 Villeurbanne, FranceUniv Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR5557 Ecologie Microbienne, 43 Bd du 11 Novembre 1918, F-69622 Villeurbanne, FranceGDEC, INRAE, UCA, F-63000 Clermont-Ferrand, FranceGDEC, INRAE, UCA, F-63000 Clermont-Ferrand, FranceGDEC, INRAE, UCA, F-63000 Clermont-Ferrand, FranceUniv Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR5557 Ecologie Microbienne, 43 Bd du 11 Novembre 1918, F-69622 Villeurbanne, FranceUniv Lyon, Université Claude Bernard Lyon 1, CNRS, INRAE, VetAgro Sup, UMR5557 Ecologie Microbienne, 43 Bd du 11 Novembre 1918, F-69622 Villeurbanne, FranceCrop varieties differ in their ability to interact with Plant Growth-Promoting Rhizobacteria (PGPR), but the genetic basis for these differences is unknown. This issue was addressed with the PGPR <i>Azospirillum baldaniorum</i> Sp245, using 187 wheat accessions. We screened the accessions based on the seedling colonization by the PGPR and the expression of the phenylpyruvate decarboxylase gene <i>ppdC</i> (for synthesis of the auxin indole-3-acetic acid), using <i>gusA</i> fusions. Then, the effects of the PGPR on the selected accessions stimulating Sp245 (or not) were compared in soil under stress. Finally, a genome-wide association approach was implemented to identify the quantitative trait loci (QTL) associated with PGPR interaction. Overall, the ancient genotypes were more effective than the modern genotypes for <i>Azospirillum</i> root colonization and <i>ppdC</i> expression. In non-sterile soil, <i>A. baldaniorum</i> Sp245 improved wheat performance for three of the four PGPR-stimulating genotypes and none of the four non-PGPR-stimulating genotypes. The genome-wide association did not identify any region for root colonization but revealed 22 regions spread on 11 wheat chromosomes for <i>ppdC</i> expression and/or <i>ppdC</i> induction rate. This is the first QTL study focusing on molecular interaction with PGPR bacteria. The molecular markers identified provide the possibility to improve the capacity of modern wheat genotypes to interact with Sp245, as well as, potentially, other <i>Azospirillum</i> strains.https://www.mdpi.com/2076-2607/11/6/1615<i>Azospirillum</i>GWASIAAPGPRQTLroot |
| spellingShingle | Jordan Valente Florence Gerin Agathe Mini Rohan Richard Jacques Le Gouis Claire Prigent-Combaret Yvan Moënne-Loccoz Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing <i>Azospirillum</i> PGPR Microorganisms <i>Azospirillum</i> GWAS IAA PGPR QTL root |
| title | Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing <i>Azospirillum</i> PGPR |
| title_full | Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing <i>Azospirillum</i> PGPR |
| title_fullStr | Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing <i>Azospirillum</i> PGPR |
| title_full_unstemmed | Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing <i>Azospirillum</i> PGPR |
| title_short | Symbiotic Variations among Wheat Genotypes and Detection of Quantitative Trait Loci for Molecular Interaction with Auxin-Producing <i>Azospirillum</i> PGPR |
| title_sort | symbiotic variations among wheat genotypes and detection of quantitative trait loci for molecular interaction with auxin producing i azospirillum i pgpr |
| topic | <i>Azospirillum</i> GWAS IAA PGPR QTL root |
| url | https://www.mdpi.com/2076-2607/11/6/1615 |
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