PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic
A method based on the polymerase chain reaction (PCR) principle was validated for detecting cow's milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spin Food I by Invitek Co., designed for the samples of animal origin. The PCR method applied util...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2006-06-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-200603-0004_pcr-based-detection-of-cow-s-milk-in-goat-and-sheep-cheeses-marketed-in-the-czech-republic.php |
| _version_ | 1797899806165696512 |
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| author | Eva Mašková Ivana Paulíčková |
| author_facet | Eva Mašková Ivana Paulíčková |
| author_sort | Eva Mašková |
| collection | DOAJ |
| description | A method based on the polymerase chain reaction (PCR) principle was validated for detecting cow's milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spin Food I by Invitek Co., designed for the samples of animal origin. The PCR method applied utilizes the sequence of the mitochondrial gene coding cytochrome b which is specific for mammals. It uses the common forward primer and the reverse primer species-specific. After electrophoresis, cow DNA was characterised by the fragment of the size of 274 bp, goat DNA by the fragment of 157 bp, and sheep DNA by the fragment of 331 bp. The detection limit of the PCR method described (1%) was determined with model samples made from pure goat cheese with a defined addition of cheese made from cow's milk. The method validated was applied in the analysis of 17 goat cheeses and 7 sheep cheeses obtained from retail trade. Products of Czech, Slovak, French, Dutch, and Italian origin were examined. The presence of undeclared cow's milk was detected in three kinds of goat cheese and in one of sheep cheese. |
| first_indexed | 2024-04-10T08:35:47Z |
| format | Article |
| id | doaj.art-52157159161647c29d178d1b2e741544 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:47Z |
| publishDate | 2006-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-52157159161647c29d178d1b2e7415442023-02-23T03:27:01ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172006-06-0124312713210.17221/3307-CJFScjf-200603-0004PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech RepublicEva Mašková0Ivana Paulíčková1Food Research Institute Prague, Prague, Czech RepublicFood Research Institute Prague, Prague, Czech RepublicA method based on the polymerase chain reaction (PCR) principle was validated for detecting cow's milk in goat and sheep cheeses. DNA was isolated from the cheeses using the isolation kit Invisorb Spin Food I by Invitek Co., designed for the samples of animal origin. The PCR method applied utilizes the sequence of the mitochondrial gene coding cytochrome b which is specific for mammals. It uses the common forward primer and the reverse primer species-specific. After electrophoresis, cow DNA was characterised by the fragment of the size of 274 bp, goat DNA by the fragment of 157 bp, and sheep DNA by the fragment of 331 bp. The detection limit of the PCR method described (1%) was determined with model samples made from pure goat cheese with a defined addition of cheese made from cow's milk. The method validated was applied in the analysis of 17 goat cheeses and 7 sheep cheeses obtained from retail trade. Products of Czech, Slovak, French, Dutch, and Italian origin were examined. The presence of undeclared cow's milk was detected in three kinds of goat cheese and in one of sheep cheese.https://cjfs.agriculturejournals.cz/artkey/cjf-200603-0004_pcr-based-detection-of-cow-s-milk-in-goat-and-sheep-cheeses-marketed-in-the-czech-republic.phppolymerase chain reactioncow's milkgoat cheesesheep cheese |
| spellingShingle | Eva Mašková Ivana Paulíčková PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic Czech Journal of Food Sciences polymerase chain reaction cow's milk goat cheese sheep cheese |
| title | PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic |
| title_full | PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic |
| title_fullStr | PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic |
| title_full_unstemmed | PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic |
| title_short | PCR-based detection of cow's milk in goat and sheep cheeses marketed in the Czech Republic |
| title_sort | pcr based detection of cow s milk in goat and sheep cheeses marketed in the czech republic |
| topic | polymerase chain reaction cow's milk goat cheese sheep cheese |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-200603-0004_pcr-based-detection-of-cow-s-milk-in-goat-and-sheep-cheeses-marketed-in-the-czech-republic.php |
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