Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in <i>Fragaria vesca</i> Genotypes of Differing Cold Tolerance
Identifying and characterizing cold responsive genes in <i>Fragaria vesca</i> associated with or responsible for low temperature tolerance is a vital part of strawberry cultivar development. In this study we have investigated the transcript levels of eight genes, two dehydrin genes, thre...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Isam Fattash Zachary Deitch Relindis Njah Nelson Osuagwu Vera Mageney Robert C. Wilson Jahn Davik Muath Alsheikh Stephen Randall |
| author_facet | Isam Fattash Zachary Deitch Relindis Njah Nelson Osuagwu Vera Mageney Robert C. Wilson Jahn Davik Muath Alsheikh Stephen Randall |
| author_sort | Isam Fattash |
| collection | DOAJ |
| description | Identifying and characterizing cold responsive genes in <i>Fragaria vesca</i> associated with or responsible for low temperature tolerance is a vital part of strawberry cultivar development. In this study we have investigated the transcript levels of eight genes, two dehydrin genes, three putative ABA-regulated genes, two cold–inducible CBF genes and the alcohol dehydrogenase gene, extracted from leaf and crown tissues of three <i>F. vesca</i> genotypes that vary in cold tolerance. Transcript levels of the CBF/DREB1 transcription factor <i>FvCBF1E</i> exhibited stronger cold up-regulation in comparison to <i>FvCBF1B.1</i> in all genotypes. Transcripts of <i>FvADH</i> were highly up-regulated in both crown and leaf tissues from all three genotypes. In the ‘ALTA’ genotype, <i>FvADH</i> transcripts were significantly higher in leaf than crown tissues and more than 10 to 20-fold greater than in the less cold-tolerant ‘NCGR1363’ and ‘FDP817’ genotypes. <i>FvGEM</i>, containing the conserved ABRE promoter element, transcript was found to be cold-regulated in crowns. Direct comparison of the kinetics of transcript and protein accumulation of dehydrins was scrutinized. In all genotypes and organs, the changes of <i>XERO2</i> transcript levels generally preceded protein changes, while levels of COR47 protein accumulation preceded the increases in <i>COR47</i> RNA in ‘ALTA’ crowns. |
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| spelling | doaj.art-5218b9b57fe242a3a06c2cc3c2bb1ac42023-11-21T23:02:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012211612410.3390/ijms22116124Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in <i>Fragaria vesca</i> Genotypes of Differing Cold ToleranceIsam Fattash0Zachary Deitch1Relindis Njah2Nelson Osuagwu3Vera Mageney4Robert C. Wilson5Jahn Davik6Muath Alsheikh7Stephen Randall8Department of Biology and Biotechnology, American University of Madaba, Madaba 11821, JordanBiology Department 723 W Michigan Street IUPUI, Indianapolis, IN 46202, USADepartment of Biotechnology, INN University, 2318 Hamar, NorwayDepartment of Clinical Medicine, University of Bergen, Postboks 7804, N-5020 Bergen, NorwayInstitute for Biology and Environmental Sciences (IBU), Carl von Ossietzky Universität Oldenburg, Carl von Ossietzky-Str. 9-11, 26111 Oldenburg, GermanyDepartment of Biotechnology, INN University, 2318 Hamar, NorwayDepartment of Molecular Plant Biology, Norwegian Institute of Bioeconomy Research, Høgskoleveien 8, N-1433 Ås, NorwayGraminor Breeding Ltd., Hommelstadveien 60, N-2322 Ridabu, NorwayBiology Department 723 W Michigan Street IUPUI, Indianapolis, IN 46202, USAIdentifying and characterizing cold responsive genes in <i>Fragaria vesca</i> associated with or responsible for low temperature tolerance is a vital part of strawberry cultivar development. In this study we have investigated the transcript levels of eight genes, two dehydrin genes, three putative ABA-regulated genes, two cold–inducible CBF genes and the alcohol dehydrogenase gene, extracted from leaf and crown tissues of three <i>F. vesca</i> genotypes that vary in cold tolerance. Transcript levels of the CBF/DREB1 transcription factor <i>FvCBF1E</i> exhibited stronger cold up-regulation in comparison to <i>FvCBF1B.1</i> in all genotypes. Transcripts of <i>FvADH</i> were highly up-regulated in both crown and leaf tissues from all three genotypes. In the ‘ALTA’ genotype, <i>FvADH</i> transcripts were significantly higher in leaf than crown tissues and more than 10 to 20-fold greater than in the less cold-tolerant ‘NCGR1363’ and ‘FDP817’ genotypes. <i>FvGEM</i>, containing the conserved ABRE promoter element, transcript was found to be cold-regulated in crowns. Direct comparison of the kinetics of transcript and protein accumulation of dehydrins was scrutinized. In all genotypes and organs, the changes of <i>XERO2</i> transcript levels generally preceded protein changes, while levels of COR47 protein accumulation preceded the increases in <i>COR47</i> RNA in ‘ALTA’ crowns.https://www.mdpi.com/1422-0067/22/11/6124dehydrinsABA-regulated genesCBF genesalcohol dehydrogenase |
| spellingShingle | Isam Fattash Zachary Deitch Relindis Njah Nelson Osuagwu Vera Mageney Robert C. Wilson Jahn Davik Muath Alsheikh Stephen Randall Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in <i>Fragaria vesca</i> Genotypes of Differing Cold Tolerance International Journal of Molecular Sciences dehydrins ABA-regulated genes CBF genes alcohol dehydrogenase |
| title | Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in <i>Fragaria vesca</i> Genotypes of Differing Cold Tolerance |
| title_full | Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in <i>Fragaria vesca</i> Genotypes of Differing Cold Tolerance |
| title_fullStr | Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in <i>Fragaria vesca</i> Genotypes of Differing Cold Tolerance |
| title_full_unstemmed | Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in <i>Fragaria vesca</i> Genotypes of Differing Cold Tolerance |
| title_short | Accumulation Dynamics of Transcripts and Proteins of Cold-Responsive Genes in <i>Fragaria vesca</i> Genotypes of Differing Cold Tolerance |
| title_sort | accumulation dynamics of transcripts and proteins of cold responsive genes in i fragaria vesca i genotypes of differing cold tolerance |
| topic | dehydrins ABA-regulated genes CBF genes alcohol dehydrogenase |
| url | https://www.mdpi.com/1422-0067/22/11/6124 |
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