Preparation, Characterization, and In Vitro Anticancer Activity Evaluation of Broccoli-Derived Extracellular Vesicle-Coated Astaxanthin Nanoparticles
Astaxanthin (AST) is a type of ketone carotenoid having significant antioxidation and anticancer abilities. However, its application is limited due to its low stability and bioavailability. In our study, poly (lactic-co-glycolic acid) (PLGA)-encapsulated AST (AST@PLGA) nanoparticles were prepared by...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/12/3955 |
| _version_ | 1797483908288217088 |
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| author | Chunmei Li Qi Song Xialian Yin Ruilong Song Gang Chen |
| author_facet | Chunmei Li Qi Song Xialian Yin Ruilong Song Gang Chen |
| author_sort | Chunmei Li |
| collection | DOAJ |
| description | Astaxanthin (AST) is a type of ketone carotenoid having significant antioxidation and anticancer abilities. However, its application is limited due to its low stability and bioavailability. In our study, poly (lactic-co-glycolic acid) (PLGA)-encapsulated AST (AST@PLGA) nanoparticles were prepared by emulsion solvent evaporation method and then further processed by ultrasound with broccoli-derived extracellular vesicles (BEVs), thereby evolving as BEV-coated AST@PLGA nanoparticles (AST@PLGA@BEVs). The preparation process and methods were optimized by three factors and three levels of response surface method to increase drug loading (DL). After optimization, the DL was increased to 6.824%, and the size, polydispersity index, and zeta potential of AST@PLGA@BEVs reached 191.60 ± 2.23 nm, 0.166, and −15.85 ± 0.92 mV, respectively. Moreover, AST@PLGA@BEVs exhibited more notable anticancer activity than AST in vitro. Collectively, these results indicate that the method of loading AST in broccoli-derived EVs is feasible and has important significance for the further development and utilization of AST as a functional food. |
| first_indexed | 2024-03-09T22:54:06Z |
| format | Article |
| id | doaj.art-521925622dbd4b90a3e33a788c3d91ec |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T22:54:06Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-521925622dbd4b90a3e33a788c3d91ec2023-11-23T18:14:21ZengMDPI AGMolecules1420-30492022-06-012712395510.3390/molecules27123955Preparation, Characterization, and In Vitro Anticancer Activity Evaluation of Broccoli-Derived Extracellular Vesicle-Coated Astaxanthin NanoparticlesChunmei Li0Qi Song1Xialian Yin2Ruilong Song3Gang Chen4College of Food Science and Engineering, Yangzhou University, Yangzhou 225009, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225009, ChinaCollege of Food Science and Engineering, Yangzhou University, Yangzhou 225009, ChinaInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaSchool of Rehabilitation Science and Engineering, University of Health and Rehabilitation Sciences, Qingdao 266024, ChinaAstaxanthin (AST) is a type of ketone carotenoid having significant antioxidation and anticancer abilities. However, its application is limited due to its low stability and bioavailability. In our study, poly (lactic-co-glycolic acid) (PLGA)-encapsulated AST (AST@PLGA) nanoparticles were prepared by emulsion solvent evaporation method and then further processed by ultrasound with broccoli-derived extracellular vesicles (BEVs), thereby evolving as BEV-coated AST@PLGA nanoparticles (AST@PLGA@BEVs). The preparation process and methods were optimized by three factors and three levels of response surface method to increase drug loading (DL). After optimization, the DL was increased to 6.824%, and the size, polydispersity index, and zeta potential of AST@PLGA@BEVs reached 191.60 ± 2.23 nm, 0.166, and −15.85 ± 0.92 mV, respectively. Moreover, AST@PLGA@BEVs exhibited more notable anticancer activity than AST in vitro. Collectively, these results indicate that the method of loading AST in broccoli-derived EVs is feasible and has important significance for the further development and utilization of AST as a functional food.https://www.mdpi.com/1420-3049/27/12/3955extracellular vesiclesastaxanthinbroccolipreparationanticancer activity |
| spellingShingle | Chunmei Li Qi Song Xialian Yin Ruilong Song Gang Chen Preparation, Characterization, and In Vitro Anticancer Activity Evaluation of Broccoli-Derived Extracellular Vesicle-Coated Astaxanthin Nanoparticles Molecules extracellular vesicles astaxanthin broccoli preparation anticancer activity |
| title | Preparation, Characterization, and In Vitro Anticancer Activity Evaluation of Broccoli-Derived Extracellular Vesicle-Coated Astaxanthin Nanoparticles |
| title_full | Preparation, Characterization, and In Vitro Anticancer Activity Evaluation of Broccoli-Derived Extracellular Vesicle-Coated Astaxanthin Nanoparticles |
| title_fullStr | Preparation, Characterization, and In Vitro Anticancer Activity Evaluation of Broccoli-Derived Extracellular Vesicle-Coated Astaxanthin Nanoparticles |
| title_full_unstemmed | Preparation, Characterization, and In Vitro Anticancer Activity Evaluation of Broccoli-Derived Extracellular Vesicle-Coated Astaxanthin Nanoparticles |
| title_short | Preparation, Characterization, and In Vitro Anticancer Activity Evaluation of Broccoli-Derived Extracellular Vesicle-Coated Astaxanthin Nanoparticles |
| title_sort | preparation characterization and in vitro anticancer activity evaluation of broccoli derived extracellular vesicle coated astaxanthin nanoparticles |
| topic | extracellular vesicles astaxanthin broccoli preparation anticancer activity |
| url | https://www.mdpi.com/1420-3049/27/12/3955 |
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