Hepatitis C virus infection causes iron deficiency in Huh7.5.1 cells.
Patients with chronic hepatitis C virus (HCV) infection frequently develop systemic iron overload, which exacerbates morbidity. Nevertheless, iron inhibits HCV replication in cell culture models and thereby exerts antiviral activity. We hypothesized that the cellular iron status is crucial for the e...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3862679?pdf=render |
| _version_ | 1818535255477321728 |
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| author | Carine Fillebeen Kostas Pantopoulos |
| author_facet | Carine Fillebeen Kostas Pantopoulos |
| author_sort | Carine Fillebeen |
| collection | DOAJ |
| description | Patients with chronic hepatitis C virus (HCV) infection frequently develop systemic iron overload, which exacerbates morbidity. Nevertheless, iron inhibits HCV replication in cell culture models and thereby exerts antiviral activity. We hypothesized that the cellular iron status is crucial for the establishment of HCV infection. We show that HCV infection of permissive Huh7.5.1 hepatoma cells promotes an iron deficient phenotype. Thus, HCV leads to increased iron regulatory protein (IRP) activity, accumulation of IRP2 and suppression of transferrin receptor 1 (TfR1) and divalent metal transporter 1 (DMT1) in the host. These data suggest that HCV regulates cellular iron levels to bypass iron-mediated inhibition in viral replication. |
| first_indexed | 2024-12-11T18:22:24Z |
| format | Article |
| id | doaj.art-521f93fa4fc943bfa7e2b992cbb12208 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T18:22:24Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-521f93fa4fc943bfa7e2b992cbb122082022-12-22T00:55:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8330710.1371/journal.pone.0083307Hepatitis C virus infection causes iron deficiency in Huh7.5.1 cells.Carine FillebeenKostas PantopoulosPatients with chronic hepatitis C virus (HCV) infection frequently develop systemic iron overload, which exacerbates morbidity. Nevertheless, iron inhibits HCV replication in cell culture models and thereby exerts antiviral activity. We hypothesized that the cellular iron status is crucial for the establishment of HCV infection. We show that HCV infection of permissive Huh7.5.1 hepatoma cells promotes an iron deficient phenotype. Thus, HCV leads to increased iron regulatory protein (IRP) activity, accumulation of IRP2 and suppression of transferrin receptor 1 (TfR1) and divalent metal transporter 1 (DMT1) in the host. These data suggest that HCV regulates cellular iron levels to bypass iron-mediated inhibition in viral replication.http://europepmc.org/articles/PMC3862679?pdf=render |
| spellingShingle | Carine Fillebeen Kostas Pantopoulos Hepatitis C virus infection causes iron deficiency in Huh7.5.1 cells. PLoS ONE |
| title | Hepatitis C virus infection causes iron deficiency in Huh7.5.1 cells. |
| title_full | Hepatitis C virus infection causes iron deficiency in Huh7.5.1 cells. |
| title_fullStr | Hepatitis C virus infection causes iron deficiency in Huh7.5.1 cells. |
| title_full_unstemmed | Hepatitis C virus infection causes iron deficiency in Huh7.5.1 cells. |
| title_short | Hepatitis C virus infection causes iron deficiency in Huh7.5.1 cells. |
| title_sort | hepatitis c virus infection causes iron deficiency in huh7 5 1 cells |
| url | http://europepmc.org/articles/PMC3862679?pdf=render |
| work_keys_str_mv | AT carinefillebeen hepatitiscvirusinfectioncausesirondeficiencyinhuh751cells AT kostaspantopoulos hepatitiscvirusinfectioncausesirondeficiencyinhuh751cells |