miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical Cancer
The overexpression of miR-218-5p in cervical cancer (CC) cell lines decreases migration, invasion and proliferation. The objective was to identify target genes of miR-218-5p and the signaling pathways and cellular processes that they regulate. The relationship between the expression of miR-218-5p an...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/13/6993 |
| _version_ | 1797479853794000896 |
|---|---|
| author | Merlin Itsel Cruz-De la Rosa Hilda Jiménez-Wences Judit Alarcón-Millán Manuel Joaquín Romero-López Carlos Alberto Castañón-Sánchez Eric Genaro Salmerón-Bárcenas Gloria Fernández-Tilapa |
| author_facet | Merlin Itsel Cruz-De la Rosa Hilda Jiménez-Wences Judit Alarcón-Millán Manuel Joaquín Romero-López Carlos Alberto Castañón-Sánchez Eric Genaro Salmerón-Bárcenas Gloria Fernández-Tilapa |
| author_sort | Merlin Itsel Cruz-De la Rosa |
| collection | DOAJ |
| description | The overexpression of miR-218-5p in cervical cancer (CC) cell lines decreases migration, invasion and proliferation. The objective was to identify target genes of miR-218-5p and the signaling pathways and cellular processes that they regulate. The relationship between the expression of miR-218-5p and RUNX2 and overall survival in CC as well as the effect of the exogenous overexpression of miR-218-5p on the level of RUNX2 were analyzed. The target gene prediction of miR-218-5p was performed in TargetScan, miRTarBase and miRDB. Predicted target genes were subjected to gene ontology (GO) and pathway enrichment analysis using the Kyoto Encyclopaedia of Genes and Genomes (KEGG). The miR-218-5p mimetic was transfected into C-33A and CaSki cells, and the miR-218-5p and RUNX2 levels were determined by RT–qPCR. Of the 118 predicted targets for miR-218-5p, 86 are involved in protein binding, and 10, including RUNX2, are involved in the upregulation of proliferation. Low miR-218-5p expression and a high level of RUNX2 are related to poor prognosis in CC. miR-218-5p overexpression is related to decreased RUNX2 expression in C-33A and CaSki cells. miR-218-5p may regulate RUNX2, and both molecules may be prognostic markers in CC. |
| first_indexed | 2024-03-09T21:51:46Z |
| format | Article |
| id | doaj.art-5222bcc154794237ae3b87908755de7c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:51:46Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-5222bcc154794237ae3b87908755de7c2023-11-23T20:06:01ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012313699310.3390/ijms23136993miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical CancerMerlin Itsel Cruz-De la Rosa0Hilda Jiménez-Wences1Judit Alarcón-Millán2Manuel Joaquín Romero-López3Carlos Alberto Castañón-Sánchez4Eric Genaro Salmerón-Bárcenas5Gloria Fernández-Tilapa6Laboratorio de Investigación Clínica, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Avenida Lázaro Cárdenas S/N, Ciudad Universitaria Sur, Col. La Haciendita, Chilpancingo, Guerrero 39087, MexicoLaboratorio de Investigación Clínica, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Avenida Lázaro Cárdenas S/N, Ciudad Universitaria Sur, Col. La Haciendita, Chilpancingo, Guerrero 39087, MexicoLaboratorio de Investigación Clínica, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Avenida Lázaro Cárdenas S/N, Ciudad Universitaria Sur, Col. La Haciendita, Chilpancingo, Guerrero 39087, MexicoLaboratorio de Investigación Clínica, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Avenida Lázaro Cárdenas S/N, Ciudad Universitaria Sur, Col. La Haciendita, Chilpancingo, Guerrero 39087, MexicoLaboratorio de Investigación Biomédica, Hospital Regional de Alta Especialidad de Oaxaca, San Bartolo Coyotepec, Oaxaca 71256, MexicoDepartamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Ciudad de México 07360, MexicoLaboratorio de Investigación Clínica, Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Avenida Lázaro Cárdenas S/N, Ciudad Universitaria Sur, Col. La Haciendita, Chilpancingo, Guerrero 39087, MexicoThe overexpression of miR-218-5p in cervical cancer (CC) cell lines decreases migration, invasion and proliferation. The objective was to identify target genes of miR-218-5p and the signaling pathways and cellular processes that they regulate. The relationship between the expression of miR-218-5p and RUNX2 and overall survival in CC as well as the effect of the exogenous overexpression of miR-218-5p on the level of RUNX2 were analyzed. The target gene prediction of miR-218-5p was performed in TargetScan, miRTarBase and miRDB. Predicted target genes were subjected to gene ontology (GO) and pathway enrichment analysis using the Kyoto Encyclopaedia of Genes and Genomes (KEGG). The miR-218-5p mimetic was transfected into C-33A and CaSki cells, and the miR-218-5p and RUNX2 levels were determined by RT–qPCR. Of the 118 predicted targets for miR-218-5p, 86 are involved in protein binding, and 10, including RUNX2, are involved in the upregulation of proliferation. Low miR-218-5p expression and a high level of RUNX2 are related to poor prognosis in CC. miR-218-5p overexpression is related to decreased RUNX2 expression in C-33A and CaSki cells. miR-218-5p may regulate RUNX2, and both molecules may be prognostic markers in CC.https://www.mdpi.com/1422-0067/23/13/6993cervical cancermiR-218-5p<i>RUNX2</i>C-33ACaSki |
| spellingShingle | Merlin Itsel Cruz-De la Rosa Hilda Jiménez-Wences Judit Alarcón-Millán Manuel Joaquín Romero-López Carlos Alberto Castañón-Sánchez Eric Genaro Salmerón-Bárcenas Gloria Fernández-Tilapa miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical Cancer International Journal of Molecular Sciences cervical cancer miR-218-5p <i>RUNX2</i> C-33A CaSki |
| title | miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical Cancer |
| title_full | miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical Cancer |
| title_fullStr | miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical Cancer |
| title_full_unstemmed | miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical Cancer |
| title_short | miR-218-5p/RUNX2 Axis Positively Regulates Proliferation and Is Associated with Poor Prognosis in Cervical Cancer |
| title_sort | mir 218 5p runx2 axis positively regulates proliferation and is associated with poor prognosis in cervical cancer |
| topic | cervical cancer miR-218-5p <i>RUNX2</i> C-33A CaSki |
| url | https://www.mdpi.com/1422-0067/23/13/6993 |
| work_keys_str_mv | AT merlinitselcruzdelarosa mir2185prunx2axispositivelyregulatesproliferationandisassociatedwithpoorprognosisincervicalcancer AT hildajimenezwences mir2185prunx2axispositivelyregulatesproliferationandisassociatedwithpoorprognosisincervicalcancer AT juditalarconmillan mir2185prunx2axispositivelyregulatesproliferationandisassociatedwithpoorprognosisincervicalcancer AT manueljoaquinromerolopez mir2185prunx2axispositivelyregulatesproliferationandisassociatedwithpoorprognosisincervicalcancer AT carlosalbertocastanonsanchez mir2185prunx2axispositivelyregulatesproliferationandisassociatedwithpoorprognosisincervicalcancer AT ericgenarosalmeronbarcenas mir2185prunx2axispositivelyregulatesproliferationandisassociatedwithpoorprognosisincervicalcancer AT gloriafernandeztilapa mir2185prunx2axispositivelyregulatesproliferationandisassociatedwithpoorprognosisincervicalcancer |