Modulation of gut microbiota by berberine and decocted Coptis chinensis Franch. in a high-fat diet-induced metabolic syndrome rat model
Objective: To investigate the effect of decocted Coptis chinensis Franch. (Huanglian Jianji, 黄连煎剂, HLJJ) and berberine on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD). Methods: Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-04-01
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| Series: | Journal of Traditional Chinese Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S209575481730090X |
| _version_ | 1797714357521481728 |
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| author | Ti Huang Xu Yan Xiaoguang Yan Fei Huang Jie Li Juncai Xiao Yi Li |
| author_facet | Ti Huang Xu Yan Xiaoguang Yan Fei Huang Jie Li Juncai Xiao Yi Li |
| author_sort | Ti Huang |
| collection | DOAJ |
| description | Objective: To investigate the effect of decocted Coptis chinensis Franch. (Huanglian Jianji, 黄连煎剂, HLJJ) and berberine on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).
Methods: Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n = 15) and HFD group (n = 45). After 8 weeks of feeding, the HFD group was further divided into three groups: a group provided with a combination of HLJJ and HFD for 4 weeks, a group provided with a combination of berberine and HFD for 4 weeks, and a group supplied only with HFD for 4 consecutive weeks. Epididymal fat weight, serum high-density lipoproteincholesterol-C (HDL-C), low-density lipoproteincholesterol-C (LDL-C) and irisin levels were measured. In addition, the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq platform.
Results: MS was successfully induced in the rats with HFD for 8 weeks. After 12 weeks, serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P < .05). The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity level. Furthermore, at the genus level, Akkermansia muciniphila (A. muciniphila), Bacteroides, and Ruminococcus were markedly increased, whereas Candidatus arthromitus, Prevotella, Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group, compared with the HFD group. The abundance of A. muciniphila, Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group, whereas, the abundance of Oscillospira was lower in the berberine group, compared with the HLJJ group.
Conclusion: HLJJ and berberine changed the gut microbiota in rats with MS. The mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different. |
| first_indexed | 2024-03-12T07:50:42Z |
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| id | doaj.art-5224fc1ed33f4f67bcf4425b770d9c5a |
| institution | Directory Open Access Journal |
| issn | 2095-7548 |
| language | English |
| last_indexed | 2024-03-12T07:50:42Z |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
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| series | Journal of Traditional Chinese Medical Sciences |
| spelling | doaj.art-5224fc1ed33f4f67bcf4425b770d9c5a2023-09-02T20:37:23ZengElsevierJournal of Traditional Chinese Medical Sciences2095-75482017-04-014214915710.1016/j.jtcms.2017.05.005Modulation of gut microbiota by berberine and decocted Coptis chinensis Franch. in a high-fat diet-induced metabolic syndrome rat modelTi Huang0Xu Yan1Xiaoguang Yan2Fei Huang3Jie Li4Juncai Xiao5Yi Li6Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, ChinaDepartment of Traditional Chinese Medicine, Beijing Hospital, Beijing 100730, ChinaDepartment of Traditional Chinese Medicine, Beijing Hospital, Beijing 100730, ChinaDepartment of Traditional Chinese Medicine, Beijing Hospital, Beijing 100730, ChinaBGI College, Shenzhen, 518083, ChinaDepartment of Endocrinology, Traditional Chinese Medicine Hospital in Pinggu District, Beijing, 101200, ChinaDepartment of Traditional Chinese Medicine, Beijing Hospital, Beijing 100730, ChinaObjective: To investigate the effect of decocted Coptis chinensis Franch. (Huanglian Jianji, 黄连煎剂, HLJJ) and berberine on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD). Methods: Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n = 15) and HFD group (n = 45). After 8 weeks of feeding, the HFD group was further divided into three groups: a group provided with a combination of HLJJ and HFD for 4 weeks, a group provided with a combination of berberine and HFD for 4 weeks, and a group supplied only with HFD for 4 consecutive weeks. Epididymal fat weight, serum high-density lipoproteincholesterol-C (HDL-C), low-density lipoproteincholesterol-C (LDL-C) and irisin levels were measured. In addition, the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq platform. Results: MS was successfully induced in the rats with HFD for 8 weeks. After 12 weeks, serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P < .05). The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity level. Furthermore, at the genus level, Akkermansia muciniphila (A. muciniphila), Bacteroides, and Ruminococcus were markedly increased, whereas Candidatus arthromitus, Prevotella, Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group, compared with the HFD group. The abundance of A. muciniphila, Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group, whereas, the abundance of Oscillospira was lower in the berberine group, compared with the HLJJ group. Conclusion: HLJJ and berberine changed the gut microbiota in rats with MS. The mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different.http://www.sciencedirect.com/science/article/pii/S209575481730090XMetabolic syndromeGut microbiotaDecocted Coptis chinensis Franch. (HLJJ)BerberineHigh-fat diet |
| spellingShingle | Ti Huang Xu Yan Xiaoguang Yan Fei Huang Jie Li Juncai Xiao Yi Li Modulation of gut microbiota by berberine and decocted Coptis chinensis Franch. in a high-fat diet-induced metabolic syndrome rat model Journal of Traditional Chinese Medical Sciences Metabolic syndrome Gut microbiota Decocted Coptis chinensis Franch. (HLJJ) Berberine High-fat diet |
| title | Modulation of gut microbiota by berberine and decocted Coptis chinensis Franch. in a high-fat diet-induced metabolic syndrome rat model |
| title_full | Modulation of gut microbiota by berberine and decocted Coptis chinensis Franch. in a high-fat diet-induced metabolic syndrome rat model |
| title_fullStr | Modulation of gut microbiota by berberine and decocted Coptis chinensis Franch. in a high-fat diet-induced metabolic syndrome rat model |
| title_full_unstemmed | Modulation of gut microbiota by berberine and decocted Coptis chinensis Franch. in a high-fat diet-induced metabolic syndrome rat model |
| title_short | Modulation of gut microbiota by berberine and decocted Coptis chinensis Franch. in a high-fat diet-induced metabolic syndrome rat model |
| title_sort | modulation of gut microbiota by berberine and decocted coptis chinensis franch in a high fat diet induced metabolic syndrome rat model |
| topic | Metabolic syndrome Gut microbiota Decocted Coptis chinensis Franch. (HLJJ) Berberine High-fat diet |
| url | http://www.sciencedirect.com/science/article/pii/S209575481730090X |
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