Electrophysiological and Behavioral Responses of <i>Apis mellifera</i> and <i>Bombus</i><i>terrestris</i> to Melon Flower Volatiles
As important pollinators, honeybees and bumblebees present a pollination behavior that is influenced by flower volatiles through the olfactory system. In this study, volatile compounds from melon flowers were isolated and identified by headspace solid-phase microextraction (HS-SPME) and gas chromato...
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MDPI AG
2022-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/11/973 |
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| author | Jiangchao Zhang Jinjia Liu Fei Gao Min Chen Yusuo Jiang Huiting Zhao Weihua Ma |
| author_facet | Jiangchao Zhang Jinjia Liu Fei Gao Min Chen Yusuo Jiang Huiting Zhao Weihua Ma |
| author_sort | Jiangchao Zhang |
| collection | DOAJ |
| description | As important pollinators, honeybees and bumblebees present a pollination behavior that is influenced by flower volatiles through the olfactory system. In this study, volatile compounds from melon flowers were isolated and identified by headspace solid-phase microextraction (HS-SPME) and gas chromatography–mass spectrometry (GC-MS), and their effects on <i>Apis mellifera</i> and <i>Bombus terrestris</i> were investigated by electroantennogram (EAG) and behavior tests (Y-tube olfactometer). The results showed that 77 volatile compounds were detected in melon flowers, among which the relative content of aldehydes was the highest (61.34%; 82.09%). <i>A. mellifera</i> showed a strong EAG response to e-2-hexenal, e-2-octenal, and 1-nonanal. <i>B. terrestris</i> showed a strong EAG response to e-2-hexenal, e-2-octenal, 2,5-dimethyl-benzaldehyde, benzaldehyde and benzenepropanal. In behavior tests, the volatiles with the highest attractive rate to <i>A. mellifera</i> were e-2-hexenal (200 μg/μL, 33.33%) and e-2-octenal (300 μg/μL, 33.33%), and those to <i>B. terrestris</i> were e-2-hexenal (10 μg/μL, 53.33%) and 2,5-dimethyl-benzaldehyde (100 μg/μL, 43.33%). E-2-hexenal and e-2-octenal were more attractive to <i>A. mellifera</i> than <i>B. terrestris</i>, respectively (10 μg/μL, 10 μg/μL, 200 μg/μL). In conclusion, the volatiles of melon flowers in facilities have certain effects on the electrophysiology and behavior of bees, which is expected to provide theoretical and technical support for the pollination of <i>A. mellifera</i> and <i>B. terrestris</i> in facilities. |
| first_indexed | 2024-03-09T18:58:33Z |
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| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T18:58:33Z |
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| spelling | doaj.art-52270718e7c04f3481985584256022862023-11-24T05:12:51ZengMDPI AGInsects2075-44502022-10-01131197310.3390/insects13110973Electrophysiological and Behavioral Responses of <i>Apis mellifera</i> and <i>Bombus</i><i>terrestris</i> to Melon Flower VolatilesJiangchao Zhang0Jinjia Liu1Fei Gao2Min Chen3Yusuo Jiang4Huiting Zhao5Weihua Ma6College of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Life Sciences, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taiyuan 030031, ChinaAs important pollinators, honeybees and bumblebees present a pollination behavior that is influenced by flower volatiles through the olfactory system. In this study, volatile compounds from melon flowers were isolated and identified by headspace solid-phase microextraction (HS-SPME) and gas chromatography–mass spectrometry (GC-MS), and their effects on <i>Apis mellifera</i> and <i>Bombus terrestris</i> were investigated by electroantennogram (EAG) and behavior tests (Y-tube olfactometer). The results showed that 77 volatile compounds were detected in melon flowers, among which the relative content of aldehydes was the highest (61.34%; 82.09%). <i>A. mellifera</i> showed a strong EAG response to e-2-hexenal, e-2-octenal, and 1-nonanal. <i>B. terrestris</i> showed a strong EAG response to e-2-hexenal, e-2-octenal, 2,5-dimethyl-benzaldehyde, benzaldehyde and benzenepropanal. In behavior tests, the volatiles with the highest attractive rate to <i>A. mellifera</i> were e-2-hexenal (200 μg/μL, 33.33%) and e-2-octenal (300 μg/μL, 33.33%), and those to <i>B. terrestris</i> were e-2-hexenal (10 μg/μL, 53.33%) and 2,5-dimethyl-benzaldehyde (100 μg/μL, 43.33%). E-2-hexenal and e-2-octenal were more attractive to <i>A. mellifera</i> than <i>B. terrestris</i>, respectively (10 μg/μL, 10 μg/μL, 200 μg/μL). In conclusion, the volatiles of melon flowers in facilities have certain effects on the electrophysiology and behavior of bees, which is expected to provide theoretical and technical support for the pollination of <i>A. mellifera</i> and <i>B. terrestris</i> in facilities.https://www.mdpi.com/2075-4450/13/11/973<i>Apis mellifera</i><i>Bombus terrestris</i>flower volatileselectroantennography (EAG)olfactory behaviormelon |
| spellingShingle | Jiangchao Zhang Jinjia Liu Fei Gao Min Chen Yusuo Jiang Huiting Zhao Weihua Ma Electrophysiological and Behavioral Responses of <i>Apis mellifera</i> and <i>Bombus</i><i>terrestris</i> to Melon Flower Volatiles Insects <i>Apis mellifera</i> <i>Bombus terrestris</i> flower volatiles electroantennography (EAG) olfactory behavior melon |
| title | Electrophysiological and Behavioral Responses of <i>Apis mellifera</i> and <i>Bombus</i><i>terrestris</i> to Melon Flower Volatiles |
| title_full | Electrophysiological and Behavioral Responses of <i>Apis mellifera</i> and <i>Bombus</i><i>terrestris</i> to Melon Flower Volatiles |
| title_fullStr | Electrophysiological and Behavioral Responses of <i>Apis mellifera</i> and <i>Bombus</i><i>terrestris</i> to Melon Flower Volatiles |
| title_full_unstemmed | Electrophysiological and Behavioral Responses of <i>Apis mellifera</i> and <i>Bombus</i><i>terrestris</i> to Melon Flower Volatiles |
| title_short | Electrophysiological and Behavioral Responses of <i>Apis mellifera</i> and <i>Bombus</i><i>terrestris</i> to Melon Flower Volatiles |
| title_sort | electrophysiological and behavioral responses of i apis mellifera i and i bombus i i terrestris i to melon flower volatiles |
| topic | <i>Apis mellifera</i> <i>Bombus terrestris</i> flower volatiles electroantennography (EAG) olfactory behavior melon |
| url | https://www.mdpi.com/2075-4450/13/11/973 |
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