A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant Failure
Kynurenine is a tryptophan metabolite linked to several inflammatory processes including transplant failure, a significant challenge in transplant medicine. The detection of small molecules such as kynurenine, however, is often complex and time consuming. Herein, we report the successful synthesis o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/6/1380 |
| _version_ | 1797488308629012480 |
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| author | Max Borgolte Isabel Quint Lars Kaiser René Csuk Hans-Peter Deigner |
| author_facet | Max Borgolte Isabel Quint Lars Kaiser René Csuk Hans-Peter Deigner |
| author_sort | Max Borgolte |
| collection | DOAJ |
| description | Kynurenine is a tryptophan metabolite linked to several inflammatory processes including transplant failure, a significant challenge in transplant medicine. The detection of small molecules such as kynurenine, however, is often complex and time consuming. Herein, we report the successful synthesis of a fluorescently labelled kynurenine derivative, showing proper fluorescence and anti-kynurenine antibody binding behavior in a magnetic bead immunoassay (MIA). The fluorescent kynurenine–rhodamine B conjugate shows a K<sub>D</sub>-value of 5.9 µM as well as IC50 values of 4.0 µM in PBS and 10.2 µM in saliva. We thus introduce a rapid test for kynurenine as a potential biomarker for kidney transplant failure. |
| first_indexed | 2024-03-10T00:00:16Z |
| format | Article |
| id | doaj.art-5233075e130c407682d3f9ecab2a8127 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T00:00:16Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-5233075e130c407682d3f9ecab2a81272023-11-23T16:17:12ZengMDPI AGDiagnostics2075-44182022-06-01126138010.3390/diagnostics12061380A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant FailureMax Borgolte0Isabel Quint1Lars Kaiser2René Csuk3Hans-Peter Deigner4Institute of Precision Medicine, Furtwangen University, Jakob-Kienzle Str. 17, 78054 Villingen-Schwenningen, GermanyInstitute of Precision Medicine, Furtwangen University, Jakob-Kienzle Str. 17, 78054 Villingen-Schwenningen, GermanyInstitute of Precision Medicine, Furtwangen University, Jakob-Kienzle Str. 17, 78054 Villingen-Schwenningen, GermanyInstitute of Organic Chemistry, Martin-Luther University Halle-Wittenberg, Kurt-Mothes-Str. 2, 06120 Halle (Saale), GermanyInstitute of Precision Medicine, Furtwangen University, Jakob-Kienzle Str. 17, 78054 Villingen-Schwenningen, GermanyKynurenine is a tryptophan metabolite linked to several inflammatory processes including transplant failure, a significant challenge in transplant medicine. The detection of small molecules such as kynurenine, however, is often complex and time consuming. Herein, we report the successful synthesis of a fluorescently labelled kynurenine derivative, showing proper fluorescence and anti-kynurenine antibody binding behavior in a magnetic bead immunoassay (MIA). The fluorescent kynurenine–rhodamine B conjugate shows a K<sub>D</sub>-value of 5.9 µM as well as IC50 values of 4.0 µM in PBS and 10.2 µM in saliva. We thus introduce a rapid test for kynurenine as a potential biomarker for kidney transplant failure.https://www.mdpi.com/2075-4418/12/6/1380transplant failuretransplant medicinebiomarkersfluorescent probesfluorescencekynurenine |
| spellingShingle | Max Borgolte Isabel Quint Lars Kaiser René Csuk Hans-Peter Deigner A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant Failure Diagnostics transplant failure transplant medicine biomarkers fluorescent probes fluorescence kynurenine |
| title | A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant Failure |
| title_full | A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant Failure |
| title_fullStr | A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant Failure |
| title_full_unstemmed | A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant Failure |
| title_short | A Fluorescence-Based Competitive Antibody Binding Assay for Kynurenine, a Potential Biomarker of Kidney Transplant Failure |
| title_sort | fluorescence based competitive antibody binding assay for kynurenine a potential biomarker of kidney transplant failure |
| topic | transplant failure transplant medicine biomarkers fluorescent probes fluorescence kynurenine |
| url | https://www.mdpi.com/2075-4418/12/6/1380 |
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