Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi

Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi

Summary: This protocol outlines a new genetic complementation strategy to investigate gene function in Trypanosoma cruzi, the parasite causing Chagas disease. We combine CRISPR-Cas9 technology with recombination of variants of the target gene containing the desired mutations that are resistant to Ca...

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Bibliographic Details
Main Authors:	Gisele Fernanda Assine Picchi-Constante, Priscila Mazzocchi Hiraiwa, Martin Marek, Vanessa Zulkievicz Rogerio, Eloise Pavão Guerra-Slompo, Christophe Romier, Nilson Ivo Tonin Zanchin
Format:	Article
Language:	English
Published:	Elsevier 2022-06-01
Series:	STAR Protocols
Subjects:	Cell Biology CRISPR Microbiology Model Organisms Molecular Biology
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722002040

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