Genomic Analysis of Endophytic <i>Bacillus</i>-Related Strains Isolated from the Medicinal Plant <i>Origanum vulgare</i> L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds
Multidrug-resistant pathogens represent a serious threat to human health. The inefficacy of traditional antibiotic drugs could be surmounted through the exploitation of natural bioactive compounds of which medicinal plants are a great reservoir. The finding that bacteria living inside plant tissues,...
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MDPI AG
2022-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/5/919 |
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| author | Giulia Semenzato Tania Alonso-Vásquez Sara Del Duca Alberto Vassallo Christopher Riccardi Marco Zaccaroni Nadia Mucci Anna Padula Giovanni Emiliani Antonio Palumbo Piccionello Anna Maria Puglia Renato Fani |
| author_facet | Giulia Semenzato Tania Alonso-Vásquez Sara Del Duca Alberto Vassallo Christopher Riccardi Marco Zaccaroni Nadia Mucci Anna Padula Giovanni Emiliani Antonio Palumbo Piccionello Anna Maria Puglia Renato Fani |
| author_sort | Giulia Semenzato |
| collection | DOAJ |
| description | Multidrug-resistant pathogens represent a serious threat to human health. The inefficacy of traditional antibiotic drugs could be surmounted through the exploitation of natural bioactive compounds of which medicinal plants are a great reservoir. The finding that bacteria living inside plant tissues, (i.e., the endophytic bacterial microbiome) can influence the synthesis of the aforementioned compounds leads to the necessity of unraveling the mechanisms involved in the determination of this symbiotic relationship. Here, we report the genome sequence of four endophytic bacterial strains isolated from the medicinal plant <i>Origanum vulgare</i> L. and able to antagonize the growth of opportunistic pathogens of cystic fibrosis patients. The in silico analysis revealed the presence of gene clusters involved in the production of antimicrobial compounds, such as paeninodin, paenilarvins, polymyxin, and paenicidin A. Endophytes’ adaptation to the plant microenvironment was evaluated through the analysis of the presence of antibiotic resistance genes in the four genomes. The diesel fuel degrading potential was also tested. Strains grew in minimum media supplemented with diesel fuel, but no <i>n</i>-alkanes degradation genes were found in their genomes, suggesting that diesel fuel degradation might occur through other steps involving enzymes catalyzing the oxidation of aromatic compounds. |
| first_indexed | 2024-03-10T03:23:02Z |
| format | Article |
| id | doaj.art-52348d3765864bee8780a05595d2c44e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:23:02Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-52348d3765864bee8780a05595d2c44e2023-11-23T12:15:00ZengMDPI AGMicroorganisms2076-26072022-04-0110591910.3390/microorganisms10050919Genomic Analysis of Endophytic <i>Bacillus</i>-Related Strains Isolated from the Medicinal Plant <i>Origanum vulgare</i> L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive CompoundsGiulia Semenzato0Tania Alonso-Vásquez1Sara Del Duca2Alberto Vassallo3Christopher Riccardi4Marco Zaccaroni5Nadia Mucci6Anna Padula7Giovanni Emiliani8Antonio Palumbo Piccionello9Anna Maria Puglia10Renato Fani11Department of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyInstitute for Environmental Protection and Research, Via Ca’ Fornacetta 9, Ozzano dell’Emilia, 40064 Bologna, ItalyInstitute for Environmental Protection and Research, Via Ca’ Fornacetta 9, Ozzano dell’Emilia, 40064 Bologna, ItalyInstitute for Sustainable Plant Protection (IPSP), National Research Council (CNR), Via Madonna del Piano 10, Sesto Fiorentino, 50019 Florence, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies-STEBICEF, University of Palermo, Viale delle Scienze Ed.17, 90128 Palermo, ItalyDepartment of Biological, Chemical and Pharmaceutical Sciences and Technologies-STEBICEF, University of Palermo, Viale delle Scienze Ed.17, 90128 Palermo, ItalyDepartment of Biology, University of Florence, Via Madonna del Piano 6, Sesto Fiorentino, 50019 Florence, ItalyMultidrug-resistant pathogens represent a serious threat to human health. The inefficacy of traditional antibiotic drugs could be surmounted through the exploitation of natural bioactive compounds of which medicinal plants are a great reservoir. The finding that bacteria living inside plant tissues, (i.e., the endophytic bacterial microbiome) can influence the synthesis of the aforementioned compounds leads to the necessity of unraveling the mechanisms involved in the determination of this symbiotic relationship. Here, we report the genome sequence of four endophytic bacterial strains isolated from the medicinal plant <i>Origanum vulgare</i> L. and able to antagonize the growth of opportunistic pathogens of cystic fibrosis patients. The in silico analysis revealed the presence of gene clusters involved in the production of antimicrobial compounds, such as paeninodin, paenilarvins, polymyxin, and paenicidin A. Endophytes’ adaptation to the plant microenvironment was evaluated through the analysis of the presence of antibiotic resistance genes in the four genomes. The diesel fuel degrading potential was also tested. Strains grew in minimum media supplemented with diesel fuel, but no <i>n</i>-alkanes degradation genes were found in their genomes, suggesting that diesel fuel degradation might occur through other steps involving enzymes catalyzing the oxidation of aromatic compounds.https://www.mdpi.com/2076-2607/10/5/919bacterial endophytesessential oilantimicrobial resistancemicrobiomeplant growth-promoting bacteria |
| spellingShingle | Giulia Semenzato Tania Alonso-Vásquez Sara Del Duca Alberto Vassallo Christopher Riccardi Marco Zaccaroni Nadia Mucci Anna Padula Giovanni Emiliani Antonio Palumbo Piccionello Anna Maria Puglia Renato Fani Genomic Analysis of Endophytic <i>Bacillus</i>-Related Strains Isolated from the Medicinal Plant <i>Origanum vulgare</i> L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds Microorganisms bacterial endophytes essential oil antimicrobial resistance microbiome plant growth-promoting bacteria |
| title | Genomic Analysis of Endophytic <i>Bacillus</i>-Related Strains Isolated from the Medicinal Plant <i>Origanum vulgare</i> L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds |
| title_full | Genomic Analysis of Endophytic <i>Bacillus</i>-Related Strains Isolated from the Medicinal Plant <i>Origanum vulgare</i> L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds |
| title_fullStr | Genomic Analysis of Endophytic <i>Bacillus</i>-Related Strains Isolated from the Medicinal Plant <i>Origanum vulgare</i> L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds |
| title_full_unstemmed | Genomic Analysis of Endophytic <i>Bacillus</i>-Related Strains Isolated from the Medicinal Plant <i>Origanum vulgare</i> L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds |
| title_short | Genomic Analysis of Endophytic <i>Bacillus</i>-Related Strains Isolated from the Medicinal Plant <i>Origanum vulgare</i> L. Revealed the Presence of Metabolic Pathways Involved in the Biosynthesis of Bioactive Compounds |
| title_sort | genomic analysis of endophytic i bacillus i related strains isolated from the medicinal plant i origanum vulgare i l revealed the presence of metabolic pathways involved in the biosynthesis of bioactive compounds |
| topic | bacterial endophytes essential oil antimicrobial resistance microbiome plant growth-promoting bacteria |
| url | https://www.mdpi.com/2076-2607/10/5/919 |
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