Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity
Abstract Objectives Biomarker levels in nasal secretions can reflect the inflammatory status of nasal mucosa and evolution of sinus disease. The aim of this study was to evaluate the relationship between local inflammatory mediator production and clinical characteristics of patients with nasal polyp...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-06-01
|
| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.794 |
| _version_ | 1818545727363612672 |
|---|---|
| author | Aleksandar Dimić Aleksandar Perić Uglješa Grgurević Jelena Sotirović Milica Labus Nenad Baletić Milanko Milojević Ivan Stanojević Danilo Vojvodić |
| author_facet | Aleksandar Dimić Aleksandar Perić Uglješa Grgurević Jelena Sotirović Milica Labus Nenad Baletić Milanko Milojević Ivan Stanojević Danilo Vojvodić |
| author_sort | Aleksandar Dimić |
| collection | DOAJ |
| description | Abstract Objectives Biomarker levels in nasal secretions can reflect the inflammatory status of nasal mucosa and evolution of sinus disease. The aim of this study was to evaluate the relationship between local inflammatory mediator production and clinical characteristics of patients with nasal polyposis (NP). Methods Thirty‐one nonaeroallergen sensitized patients with NP (NANP), 29 aeroallergen sensitized patients with NP (ANP), and 30 subjects without inflammation of nasal mucosa as controls (C) entered this prospective, cross‐sectional study. Clinical parameters (symptoms, endoscopic, and radiological findings) were assessed. The concentrations of heat shock protein 70 (HSP70), eosinophil cationic protein (ECP), tryptase, substance P and Clara cell protein 16 (CC16) were measured in the nasal secretion samples of all participants by ELISA method. Results Our results showed higher concentrations of HSP70, ECP, and tryptase in ANP than in NANP and C (p < .001 for all markers). On the other hand, levels of CC16 were significantly higher in C than in NANP and ANP groups (p < .001; p < .001, respectively). We found positive correlations between HSP70, ECP, tryptase, and substance P levels and nasal symptom score in patients with NP. Also, HSP70, ECP, tryptase, and substance P showed different levels of positive correlation among themselves, with HSP70 showing highest positive correlation with ECP. Finally, relatively strong negative correlations were found between the levels of CC16 and nasal symptoms, as well as between the CC16 levels and levels of other four mediators in nasal fluid. Conclusion HSP70, ECP, tryptase, and substance P might play a role in the pathogenesis of NP. The results suggest that chronic inflammation in NP involves a self‐sustaining local release of HSP70, ECP, and tryptase, independent of aeroallergen stimulation of the mucosal layer, although the production of these mediators is higher in aeroallergen sensitized NP patients. |
| first_indexed | 2024-12-12T07:43:50Z |
| format | Article |
| id | doaj.art-52354d592a4f454b82bacd49b076ace6 |
| institution | Directory Open Access Journal |
| issn | 2378-8038 |
| language | English |
| last_indexed | 2024-12-12T07:43:50Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj.art-52354d592a4f454b82bacd49b076ace62022-12-22T00:32:42ZengWileyLaryngoscope Investigative Otolaryngology2378-80382022-06-017367167810.1002/lio2.794Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivityAleksandar Dimić0Aleksandar Perić1Uglješa Grgurević2Jelena Sotirović3Milica Labus4Nenad Baletić5Milanko Milojević6Ivan Stanojević7Danilo Vojvodić8Department of Otorhinolaryngology Military Medical Academy Belgrade SerbiaDepartment of Otorhinolaryngology, Faculty of Medicine of the Military Medical Academy University of Defence Belgrade SerbiaDepartment of Otorhinolaryngology Military Medical Academy Belgrade SerbiaDepartment of Otorhinolaryngology, Faculty of Medicine of the Military Medical Academy University of Defence Belgrade SerbiaDepartment of Otorhinolaryngology Military Medical Academy Belgrade SerbiaDepartment of Otorhinolaryngology, Faculty of Medicine of the Military Medical Academy University of Defence Belgrade SerbiaDepartment of Otorhinolaryngology, Faculty of Medicine of the Military Medical Academy University of Defence Belgrade SerbiaInstitute for Medical Research, Division of Clinical and Experimental Immunology Faculty of Medicine of the Military Medical Academy Belgrade SerbiaInstitute for Medical Research, Division of Clinical and Experimental Immunology Faculty of Medicine of the Military Medical Academy Belgrade SerbiaAbstract Objectives Biomarker levels in nasal secretions can reflect the inflammatory status of nasal mucosa and evolution of sinus disease. The aim of this study was to evaluate the relationship between local inflammatory mediator production and clinical characteristics of patients with nasal polyposis (NP). Methods Thirty‐one nonaeroallergen sensitized patients with NP (NANP), 29 aeroallergen sensitized patients with NP (ANP), and 30 subjects without inflammation of nasal mucosa as controls (C) entered this prospective, cross‐sectional study. Clinical parameters (symptoms, endoscopic, and radiological findings) were assessed. The concentrations of heat shock protein 70 (HSP70), eosinophil cationic protein (ECP), tryptase, substance P and Clara cell protein 16 (CC16) were measured in the nasal secretion samples of all participants by ELISA method. Results Our results showed higher concentrations of HSP70, ECP, and tryptase in ANP than in NANP and C (p < .001 for all markers). On the other hand, levels of CC16 were significantly higher in C than in NANP and ANP groups (p < .001; p < .001, respectively). We found positive correlations between HSP70, ECP, tryptase, and substance P levels and nasal symptom score in patients with NP. Also, HSP70, ECP, tryptase, and substance P showed different levels of positive correlation among themselves, with HSP70 showing highest positive correlation with ECP. Finally, relatively strong negative correlations were found between the levels of CC16 and nasal symptoms, as well as between the CC16 levels and levels of other four mediators in nasal fluid. Conclusion HSP70, ECP, tryptase, and substance P might play a role in the pathogenesis of NP. The results suggest that chronic inflammation in NP involves a self‐sustaining local release of HSP70, ECP, and tryptase, independent of aeroallergen stimulation of the mucosal layer, although the production of these mediators is higher in aeroallergen sensitized NP patients.https://doi.org/10.1002/lio2.794eosinophil cationic proteinheat‐shock proteinsinflammationnasal polypssubstance PTryptases |
| spellingShingle | Aleksandar Dimić Aleksandar Perić Uglješa Grgurević Jelena Sotirović Milica Labus Nenad Baletić Milanko Milojević Ivan Stanojević Danilo Vojvodić Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity Laryngoscope Investigative Otolaryngology eosinophil cationic protein heat‐shock proteins inflammation nasal polyps substance P Tryptases |
| title | Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity |
| title_full | Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity |
| title_fullStr | Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity |
| title_full_unstemmed | Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity |
| title_short | Different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity |
| title_sort | different levels of mucus inflammatory mediators in nasal polyposis with and without aeroallergen sensitivity |
| topic | eosinophil cationic protein heat‐shock proteins inflammation nasal polyps substance P Tryptases |
| url | https://doi.org/10.1002/lio2.794 |
| work_keys_str_mv | AT aleksandardimic differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity AT aleksandarperic differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity AT ugljesagrgurevic differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity AT jelenasotirovic differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity AT milicalabus differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity AT nenadbaletic differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity AT milankomilojevic differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity AT ivanstanojevic differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity AT danilovojvodic differentlevelsofmucusinflammatorymediatorsinnasalpolyposiswithandwithoutaeroallergensensitivity |