Cytotoxic Effects of Dibromoisatin Derivatives on Hela and HepG2 Cell Lines Using MTT Assay
Background and purpose: Tumors are one of the most important threats to human life and improving the drugs used in their treatment is always necessary. Isatin is found in many plants and also synthesized through different methods. Many studies have been done on Isatin ring, including the construct...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2017-08-01
|
| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-8870-en.html |
| _version_ | 1797943708756213760 |
|---|---|
| author | Mohammad Shokrzadeh Saied Emami Melika Amirzadeh Mona Modanloo |
| author_facet | Mohammad Shokrzadeh Saied Emami Melika Amirzadeh Mona Modanloo |
| author_sort | Mohammad Shokrzadeh |
| collection | DOAJ |
| description | Background and purpose: Tumors are one of the most important threats to human life and improving the drugs used in their treatment is always necessary. Isatin is found in many plants and also synthesized through different methods. Many studies have been done on Isatin ring, including the construction of Dibromoisatin derivatives that exhibit anti-cancer effects. The aim of this study was to determine the cytotoxic effects of Dibromoisatin derivatives on cervical and liver cancer cells.
Materials and methods: In this study, 10 Dibromoisatin derivative made at concentrations of 1, 50, 100, 200, and 400 µg/mL were tested against cervical (HeLa) and liver cancer cell lines (HepG2). The cell viability was assessed by MTT assay and the IC50 value was calculated in PRISM software.
Results: Comparing the cytotoxic effects of Dibromoisatin derivatives showed that the highest cytotoxic effects in both cell lines was detected in composition No. 9 with IC50 value of HepG2= 1.194 and IC50 value of HeLa= 0.025.
Conclusion: These derivatives were more effective against liver cancer cells and some Dibromoisatin derivatives were found to have high toxicity against cancer cells, therefore, they could be of great benefit in improving the drugs used in chemotherapy. |
| first_indexed | 2024-04-10T20:28:28Z |
| format | Article |
| id | doaj.art-5236287bd3264945a6cc6230baa168a8 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:28:28Z |
| publishDate | 2017-08-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-5236287bd3264945a6cc6230baa168a82023-01-25T07:50:58ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792017-08-01271512431Cytotoxic Effects of Dibromoisatin Derivatives on Hela and HepG2 Cell Lines Using MTT AssayMohammad Shokrzadeh0Saied Emami1Melika Amirzadeh2Mona Modanloo3 Associate Professor, Department of Toxicology and Pharmacology, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran Professor, Department of Pharmacology, Mazandaran University of Medical Sciences, Sari, Iran Pharmacy student, Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran PhD Student in Toxicology, Pharmaceutical Sciences Researches Center, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Tumors are one of the most important threats to human life and improving the drugs used in their treatment is always necessary. Isatin is found in many plants and also synthesized through different methods. Many studies have been done on Isatin ring, including the construction of Dibromoisatin derivatives that exhibit anti-cancer effects. The aim of this study was to determine the cytotoxic effects of Dibromoisatin derivatives on cervical and liver cancer cells. Materials and methods: In this study, 10 Dibromoisatin derivative made at concentrations of 1, 50, 100, 200, and 400 µg/mL were tested against cervical (HeLa) and liver cancer cell lines (HepG2). The cell viability was assessed by MTT assay and the IC50 value was calculated in PRISM software. Results: Comparing the cytotoxic effects of Dibromoisatin derivatives showed that the highest cytotoxic effects in both cell lines was detected in composition No. 9 with IC50 value of HepG2= 1.194 and IC50 value of HeLa= 0.025. Conclusion: These derivatives were more effective against liver cancer cells and some Dibromoisatin derivatives were found to have high toxicity against cancer cells, therefore, they could be of great benefit in improving the drugs used in chemotherapy.http://jmums.mazums.ac.ir/article-1-8870-en.htmldibromoisatincytotoxicityliver cancercervical cancer |
| spellingShingle | Mohammad Shokrzadeh Saied Emami Melika Amirzadeh Mona Modanloo Cytotoxic Effects of Dibromoisatin Derivatives on Hela and HepG2 Cell Lines Using MTT Assay Journal of Mazandaran University of Medical Sciences dibromoisatin cytotoxicity liver cancer cervical cancer |
| title | Cytotoxic Effects of Dibromoisatin Derivatives on Hela and HepG2 Cell Lines Using MTT Assay |
| title_full | Cytotoxic Effects of Dibromoisatin Derivatives on Hela and HepG2 Cell Lines Using MTT Assay |
| title_fullStr | Cytotoxic Effects of Dibromoisatin Derivatives on Hela and HepG2 Cell Lines Using MTT Assay |
| title_full_unstemmed | Cytotoxic Effects of Dibromoisatin Derivatives on Hela and HepG2 Cell Lines Using MTT Assay |
| title_short | Cytotoxic Effects of Dibromoisatin Derivatives on Hela and HepG2 Cell Lines Using MTT Assay |
| title_sort | cytotoxic effects of dibromoisatin derivatives on hela and hepg2 cell lines using mtt assay |
| topic | dibromoisatin cytotoxicity liver cancer cervical cancer |
| url | http://jmums.mazums.ac.ir/article-1-8870-en.html |
| work_keys_str_mv | AT mohammadshokrzadeh cytotoxiceffectsofdibromoisatinderivativesonhelaandhepg2celllinesusingmttassay AT saiedemami cytotoxiceffectsofdibromoisatinderivativesonhelaandhepg2celllinesusingmttassay AT melikaamirzadeh cytotoxiceffectsofdibromoisatinderivativesonhelaandhepg2celllinesusingmttassay AT monamodanloo cytotoxiceffectsofdibromoisatinderivativesonhelaandhepg2celllinesusingmttassay |