Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach
Abstract Background Pepper is one of the most cultivated crops worldwide, but is sensitive to salinity. This sensitivity is dependent on varieties and our knowledge about how they can face such stress is limited, mainly according to a molecular point of view. This is the main reason why we decided t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-04-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-02938-2 |
| _version_ | 1818966138865844224 |
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| author | Lidia López-Serrano Ángeles Calatayud Salvador López-Galarza Ramón Serrano Eduardo Bueso |
| author_facet | Lidia López-Serrano Ángeles Calatayud Salvador López-Galarza Ramón Serrano Eduardo Bueso |
| author_sort | Lidia López-Serrano |
| collection | DOAJ |
| description | Abstract Background Pepper is one of the most cultivated crops worldwide, but is sensitive to salinity. This sensitivity is dependent on varieties and our knowledge about how they can face such stress is limited, mainly according to a molecular point of view. This is the main reason why we decided to develop this transcriptomic analysis. Tolerant and sensitive accessions, respectively called A25 and A6, were grown for 14 days under control conditions and irrigated with 70 mM of NaCl. Biomass, different physiological parameters and differentially expressed genes were analysed to give response to differential salinity mechanisms between both accessions. Results The genetic changes found between the accessions under both control and stress conditions could explain the physiological behaviour in A25 by the decrease of osmotic potential that could be due mainly to an increase in potassium and proline accumulation, improved growth (e.g. expansins), more efficient starch accumulation (e.g. BAM1), ion homeostasis (e.g. CBL9, HAI3, BASS1), photosynthetic protection (e.g. FIB1A, TIL, JAR1) and antioxidant activity (e.g. PSDS3, SnRK2.10). In addition, misregulation of ABA signalling (e.g. HAB1, ERD4, HAI3) and other stress signalling genes (e.g. JAR1) would appear crucial to explain the different sensitivity to NaCl in both accessions. Conclusions After analysing the physiological behaviour and transcriptomic results, we have concluded that A25 accession utilizes different strategies to cope better salt stress, being ABA-signalling a pivotal point of regulation. However, other strategies, such as the decrease in osmotic potential to preserve water status in leaves seem to be important to explain the defence response to salinity in pepper A25 plants. |
| first_indexed | 2024-12-20T13:28:09Z |
| format | Article |
| id | doaj.art-5236d73cfe544c8da6bdd590c3820d63 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-20T13:28:09Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-5236d73cfe544c8da6bdd590c3820d632022-12-21T19:39:11ZengBMCBMC Plant Biology1471-22292021-04-0121111710.1186/s12870-021-02938-2Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approachLidia López-Serrano0Ángeles Calatayud1Salvador López-Galarza2Ramón Serrano3Eduardo Bueso4Centro de Citricultura y Producción Vegetal, Departamento de Horticultura, Instituto Valenciano de Investigaciones AgrariasCentro de Citricultura y Producción Vegetal, Departamento de Horticultura, Instituto Valenciano de Investigaciones AgrariasDepartamento de Producción Vegetal, Universitat Politècnica de ValènciaInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.CInstituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-C.S.I.CAbstract Background Pepper is one of the most cultivated crops worldwide, but is sensitive to salinity. This sensitivity is dependent on varieties and our knowledge about how they can face such stress is limited, mainly according to a molecular point of view. This is the main reason why we decided to develop this transcriptomic analysis. Tolerant and sensitive accessions, respectively called A25 and A6, were grown for 14 days under control conditions and irrigated with 70 mM of NaCl. Biomass, different physiological parameters and differentially expressed genes were analysed to give response to differential salinity mechanisms between both accessions. Results The genetic changes found between the accessions under both control and stress conditions could explain the physiological behaviour in A25 by the decrease of osmotic potential that could be due mainly to an increase in potassium and proline accumulation, improved growth (e.g. expansins), more efficient starch accumulation (e.g. BAM1), ion homeostasis (e.g. CBL9, HAI3, BASS1), photosynthetic protection (e.g. FIB1A, TIL, JAR1) and antioxidant activity (e.g. PSDS3, SnRK2.10). In addition, misregulation of ABA signalling (e.g. HAB1, ERD4, HAI3) and other stress signalling genes (e.g. JAR1) would appear crucial to explain the different sensitivity to NaCl in both accessions. Conclusions After analysing the physiological behaviour and transcriptomic results, we have concluded that A25 accession utilizes different strategies to cope better salt stress, being ABA-signalling a pivotal point of regulation. However, other strategies, such as the decrease in osmotic potential to preserve water status in leaves seem to be important to explain the defence response to salinity in pepper A25 plants.https://doi.org/10.1186/s12870-021-02938-2Abscisic acidGrowthIon homeostasisPhotosynthesisSalt stressTolerant accessions |
| spellingShingle | Lidia López-Serrano Ángeles Calatayud Salvador López-Galarza Ramón Serrano Eduardo Bueso Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach BMC Plant Biology Abscisic acid Growth Ion homeostasis Photosynthesis Salt stress Tolerant accessions |
| title | Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach |
| title_full | Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach |
| title_fullStr | Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach |
| title_full_unstemmed | Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach |
| title_short | Uncovering salt tolerance mechanisms in pepper plants: a physiological and transcriptomic approach |
| title_sort | uncovering salt tolerance mechanisms in pepper plants a physiological and transcriptomic approach |
| topic | Abscisic acid Growth Ion homeostasis Photosynthesis Salt stress Tolerant accessions |
| url | https://doi.org/10.1186/s12870-021-02938-2 |
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