Choice of DNA extraction method affects stool microbiome recovery and subsequent phenotypic association analyses

Choice of DNA extraction method affects stool microbiome recovery and subsequent phenotypic association analyses

Abstract The lack of standardization in the methods of DNA extraction from fecal samples represents the major source of experimental variation in the microbiome research field. In this study, we aimed to compare the metagenomic profiles and microbiome–phenotype associations obtained by applying two...

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Bibliographic Details
Main Authors: Asier Fernández-Pato, Trishla Sinha, Ranko Gacesa, Sergio Andreu-Sánchez, Milla F. Brandao Gois, Jody Gelderloos-Arends, Dianne B. H. Jansen, Marloes Kruk, Martin Jaeger, Leo A. B. Joosten, Mihai G. Netea, Rinse K. Weersma, Cisca Wijmenga, Hermie J. M. Harmsen, Jingyuan Fu, Alexandra Zhernakova, Alexander Kurilshikov
Format: Article
Language:English
Published: Nature Portfolio 2024-02-01
Series:Scientific Reports
Subjects:
Gut microbiome
Fecal sample
DNA extraction
Shotgun metagenomics
Online Access:https://doi.org/10.1038/s41598-024-54353-w

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