Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b
Long non-coding RNAs (lncRNAs) are involved in shaping chromosome conformation and regulation of preimplantation development. However, the role of lncRNA during somatic cell nuclear transfer (SCNT) reprogramming remains largely unknown. In the present study, we identified 114 upregulated lncRNAs in...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Molecular Therapy: Nucleic Acids |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253121000512 |
| _version_ | 1818865261947650048 |
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| author | Mingtian Deng Yongjie Wan Baobao Chen Xiangpeng Dai Zifei Liu Yingnan Yang Yu Cai Yanli Zhang Feng Wang |
| author_facet | Mingtian Deng Yongjie Wan Baobao Chen Xiangpeng Dai Zifei Liu Yingnan Yang Yu Cai Yanli Zhang Feng Wang |
| author_sort | Mingtian Deng |
| collection | DOAJ |
| description | Long non-coding RNAs (lncRNAs) are involved in shaping chromosome conformation and regulation of preimplantation development. However, the role of lncRNA during somatic cell nuclear transfer (SCNT) reprogramming remains largely unknown. In the present study, we identified 114 upregulated lncRNAs in the 8-cell SCNT embryos as candidate key molecules involved in nuclear reprogramming in goat. We found that H3K4me3 was an epigenetic barrier in goat nuclear reprogramming that and injection of Kdm5b mRNA greatly improved SCNT embryos development through removal of H3K4me3. We further reported that knockdown of lnc_3712 increased the expression of Kdm5b, which led to H3K4me3 demethylation. Of note, the development of goat SCNT embryos was improved when lnc_3712 was knocked down, whereas the blastocyst rate showed no difference in lnc_3712 and Kdm5b double knockdown SCNT embryos compared with the negative control SCNT embryos. Specifically, in lnc_3712 knockdown SCNT embryos, partial of the transcriptional activity and the expression of critical embryonic genes (Wee1, Ctsb, and Ybx1) were similar with that of in vitro fertilization embryos. Therefore, our results elucidate the critical role of lnc_3712 in regulating the development of goat SCNT embryos via repressing Kdm5b, which advances our current understanding of the role of lncRNAs during nuclear reprogramming. |
| first_indexed | 2024-12-19T10:44:45Z |
| format | Article |
| id | doaj.art-5239734ae03f4653841fe28db7ebe45a |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-19T10:44:45Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-5239734ae03f4653841fe28db7ebe45a2022-12-21T20:25:19ZengElsevierMolecular Therapy: Nucleic Acids2162-25312021-06-01245466Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5bMingtian Deng0Yongjie Wan1Baobao Chen2Xiangpeng Dai3Zifei Liu4Yingnan Yang5Yu Cai6Yanli Zhang7Feng Wang8Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaKey Laboratory of Organ Regeneration and Transplantation of Ministry of Education, First Hospital, Jilin University, Changchun, Jilin 130021, ChinaJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author: Yanli Zhang, PhD, Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author: Feng Wang, PhD, Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.Long non-coding RNAs (lncRNAs) are involved in shaping chromosome conformation and regulation of preimplantation development. However, the role of lncRNA during somatic cell nuclear transfer (SCNT) reprogramming remains largely unknown. In the present study, we identified 114 upregulated lncRNAs in the 8-cell SCNT embryos as candidate key molecules involved in nuclear reprogramming in goat. We found that H3K4me3 was an epigenetic barrier in goat nuclear reprogramming that and injection of Kdm5b mRNA greatly improved SCNT embryos development through removal of H3K4me3. We further reported that knockdown of lnc_3712 increased the expression of Kdm5b, which led to H3K4me3 demethylation. Of note, the development of goat SCNT embryos was improved when lnc_3712 was knocked down, whereas the blastocyst rate showed no difference in lnc_3712 and Kdm5b double knockdown SCNT embryos compared with the negative control SCNT embryos. Specifically, in lnc_3712 knockdown SCNT embryos, partial of the transcriptional activity and the expression of critical embryonic genes (Wee1, Ctsb, and Ybx1) were similar with that of in vitro fertilization embryos. Therefore, our results elucidate the critical role of lnc_3712 in regulating the development of goat SCNT embryos via repressing Kdm5b, which advances our current understanding of the role of lncRNAs during nuclear reprogramming.http://www.sciencedirect.com/science/article/pii/S2162253121000512somatic cell nuclear transferreprogramminglong non-coding RNAKdm5bH3K4me3goat |
| spellingShingle | Mingtian Deng Yongjie Wan Baobao Chen Xiangpeng Dai Zifei Liu Yingnan Yang Yu Cai Yanli Zhang Feng Wang Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b Molecular Therapy: Nucleic Acids somatic cell nuclear transfer reprogramming long non-coding RNA Kdm5b H3K4me3 goat |
| title | Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b |
| title_full | Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b |
| title_fullStr | Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b |
| title_full_unstemmed | Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b |
| title_short | Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b |
| title_sort | long non coding rna lnc 3712 impedes nuclear reprogramming via repressing kdm5b |
| topic | somatic cell nuclear transfer reprogramming long non-coding RNA Kdm5b H3K4me3 goat |
| url | http://www.sciencedirect.com/science/article/pii/S2162253121000512 |
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