Diversity of Helicobacter pylori isolates expressing the major protein antigens and inducing specific antibodies in host(幽门螺杆菌临床菌株主要蛋白抗原表达及其诱导宿主产生抗体差异性的研究)
建立了基于幽门螺杆菌重组蛋白抗原rUreB、rHpaA、rVacA、rCagA1、rNapA、rFlaA和rFlaB的ELISAs ,用于检测幽门螺杆菌临床菌株上述抗原表达率和幽门螺杆菌感染病人血清IgG阳性率,另采用PCR检测临床菌株中上述抗原编码基因携带率,以期为选择幽门螺杆菌基因工程疫苗抗原提供依据.实验结果显示,rUreB、rHpaA、rVacA、rCagA1、rNapA、rFlaA 和 rFlaB 表达量分别约为细菌总蛋白的35%、32%、15%、23%、56%、25%和20%.各重组抗原均能与商品化幽门螺杆菌抗血清(DAKO)产生阳性Western Bolt结果,免疫家兔能产生特异...
| Main Authors: | , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Zhejiang University Press
2009-01-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2009.01.019 |