Massively parallel DNA sequencing: the new frontier in biogeography
The advent of Sanger sequencing represented a scientific break-through that greatly advanced biogeographic studies. However, this technology has several limitations that have hampered more advanced studies in the field. The development of novel techniques which more fully exploit the potential of Ma...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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International Biogeography Society
2013-04-01
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| Series: | Frontiers of Biogeography |
| Subjects: | |
| Online Access: | http://escholarship.org/uc/item/7xv39142 |
| _version_ | 1819241388717375488 |
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| author | Luiz A. Rocha Moisés A. Bernal Michelle R. Gaither Michael E. Alfaro |
| author_facet | Luiz A. Rocha Moisés A. Bernal Michelle R. Gaither Michael E. Alfaro |
| author_sort | Luiz A. Rocha |
| collection | DOAJ |
| description | The advent of Sanger sequencing represented a scientific break-through that greatly advanced biogeographic studies. However, this technology has several limitations that have hampered more advanced studies in the field. The development of novel techniques which more fully exploit the potential of Massively Parallel Sequencing (MPS) to deliver sequence data at a fraction of the cost of Sanger sequencing promises to revolutionize biogeographic studies. Approaches like Restriction-site Associated DNA sequencing (RADseq) and UltraConserved Element (UCE) sequencing enable the collection of unprecedented amounts of data for multi-locus studies of population genetics and phylogenetics respectively, which in turn can be used for biogeographic analysis. Here we review those and other methods related to MPS, and provide examples of how they can be used in tropical Atlantic biogeography. |
| first_indexed | 2024-12-23T14:23:07Z |
| format | Article |
| id | doaj.art-523e16baa5884bb290573b7ba377189e |
| institution | Directory Open Access Journal |
| issn | 1948-6596 |
| language | English |
| last_indexed | 2024-12-23T14:23:07Z |
| publishDate | 2013-04-01 |
| publisher | International Biogeography Society |
| record_format | Article |
| series | Frontiers of Biogeography |
| spelling | doaj.art-523e16baa5884bb290573b7ba377189e2022-12-21T17:43:44ZengInternational Biogeography SocietyFrontiers of Biogeography1948-65962013-04-0151Massively parallel DNA sequencing: the new frontier in biogeographyLuiz A. RochaMoisés A. BernalMichelle R. GaitherMichael E. AlfaroThe advent of Sanger sequencing represented a scientific break-through that greatly advanced biogeographic studies. However, this technology has several limitations that have hampered more advanced studies in the field. The development of novel techniques which more fully exploit the potential of Massively Parallel Sequencing (MPS) to deliver sequence data at a fraction of the cost of Sanger sequencing promises to revolutionize biogeographic studies. Approaches like Restriction-site Associated DNA sequencing (RADseq) and UltraConserved Element (UCE) sequencing enable the collection of unprecedented amounts of data for multi-locus studies of population genetics and phylogenetics respectively, which in turn can be used for biogeographic analysis. Here we review those and other methods related to MPS, and provide examples of how they can be used in tropical Atlantic biogeography.http://escholarship.org/uc/item/7xv39142next-generation sequencing, phylogeography, phylogenetics, genomics, hybridization |
| spellingShingle | Luiz A. Rocha Moisés A. Bernal Michelle R. Gaither Michael E. Alfaro Massively parallel DNA sequencing: the new frontier in biogeography Frontiers of Biogeography next-generation sequencing, phylogeography, phylogenetics, genomics, hybridization |
| title | Massively parallel DNA sequencing: the new frontier in biogeography |
| title_full | Massively parallel DNA sequencing: the new frontier in biogeography |
| title_fullStr | Massively parallel DNA sequencing: the new frontier in biogeography |
| title_full_unstemmed | Massively parallel DNA sequencing: the new frontier in biogeography |
| title_short | Massively parallel DNA sequencing: the new frontier in biogeography |
| title_sort | massively parallel dna sequencing the new frontier in biogeography |
| topic | next-generation sequencing, phylogeography, phylogenetics, genomics, hybridization |
| url | http://escholarship.org/uc/item/7xv39142 |
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