IL-25 promotes trophoblast proliferation and invasion via binding with IL-17RB and associated with PE
Introduction: Interleukin-25 is a Th2 interleukin and has been shown to influence cell behavior. This study aims to illustrate its affection on extravillous trophoblasts function and association with PE. Methods qPCR and immunohistochemistry demonstrate IL-25 and IL-17RB expression. CCK-8 test and t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-07-01
|
| Series: | Hypertension in Pregnancy |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10641955.2021.1950177 |
| _version_ | 1797681511019839488 |
|---|---|
| author | Siyu Liu Yi Sun Yao Tang Rong Hu Qiongjie Zhou Xiaotian Li |
| author_facet | Siyu Liu Yi Sun Yao Tang Rong Hu Qiongjie Zhou Xiaotian Li |
| author_sort | Siyu Liu |
| collection | DOAJ |
| description | Introduction: Interleukin-25 is a Th2 interleukin and has been shown to influence cell behavior. This study aims to illustrate its affection on extravillous trophoblasts function and association with PE. Methods qPCR and immunohistochemistry demonstrate IL-25 and IL-17RB expression. CCK-8 test and transwell were to access the behavior of HTR8 in the presence or absence of IL-25, IL-25 neutralization antibody. Results EVTs and HTR8 express IL-25 and IL-17RB. IL-25 promotes proliferation and invasion (p< 0.05),which was abolished in the presence of IL-25Ab (p< 0.05). Conclusion: IL-25 may contribute to promote trophoblast invasion and proliferation and abnormal decline of IL-25 might be associated with PE. Methods: qPCR and immunohistochemistry (IHC) were used to demonstrate IL-25 and its receptor IL-17RB expression in primary human trophoblasts of normal first- and third- trimester, as well as third-trimester of PE. CCK-8 test and transwell invasion system in vitro were applied separately to access the behavior of trophoblast cell line (HTR8) in the presence or absence of IL-25, IL-25 neutralization antibody (IL-25 Ab). Results: EVTs and HTR8 express IL-25 and IL-17RB. The expressions increase in third-trimester during normal pregnancy. In PE, both of IL-25 and IL-17RB expressions decrease (p< 0.05). IL-25 for 24 h promoted HTR8 proliferation and invasion (p< 0.05). The effect was abolished in the presence of IL-25Ab (p< 0.05). Conclusion: This study demonstrates that IL-25 may contribute to promote trophoblast invasion and proliferation and abnormal decline of IL-25 might be associated with PE. |
| first_indexed | 2024-03-11T23:45:53Z |
| format | Article |
| id | doaj.art-523e3af5e8c84864b72cb07484b437e0 |
| institution | Directory Open Access Journal |
| issn | 1064-1955 1525-6065 |
| language | English |
| last_indexed | 2024-03-11T23:45:53Z |
| publishDate | 2021-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hypertension in Pregnancy |
| spelling | doaj.art-523e3af5e8c84864b72cb07484b437e02023-09-19T09:24:44ZengTaylor & Francis GroupHypertension in Pregnancy1064-19551525-60652021-07-0140320921710.1080/10641955.2021.19501771950177IL-25 promotes trophoblast proliferation and invasion via binding with IL-17RB and associated with PESiyu Liu0Yi Sun1Yao Tang2Rong Hu3Qiongjie Zhou4Xiaotian Li5Obstetrics and Gynecology Hospital of Fudan UniversityObstetrics and Gynecology Hospital of Fudan UniversityUiT the Arctic University of NorwayUiT the Arctic University of NorwayUiT the Arctic University of NorwayObstetrics and Gynecology Hospital of Fudan UniversityIntroduction: Interleukin-25 is a Th2 interleukin and has been shown to influence cell behavior. This study aims to illustrate its affection on extravillous trophoblasts function and association with PE. Methods qPCR and immunohistochemistry demonstrate IL-25 and IL-17RB expression. CCK-8 test and transwell were to access the behavior of HTR8 in the presence or absence of IL-25, IL-25 neutralization antibody. Results EVTs and HTR8 express IL-25 and IL-17RB. IL-25 promotes proliferation and invasion (p< 0.05),which was abolished in the presence of IL-25Ab (p< 0.05). Conclusion: IL-25 may contribute to promote trophoblast invasion and proliferation and abnormal decline of IL-25 might be associated with PE. Methods: qPCR and immunohistochemistry (IHC) were used to demonstrate IL-25 and its receptor IL-17RB expression in primary human trophoblasts of normal first- and third- trimester, as well as third-trimester of PE. CCK-8 test and transwell invasion system in vitro were applied separately to access the behavior of trophoblast cell line (HTR8) in the presence or absence of IL-25, IL-25 neutralization antibody (IL-25 Ab). Results: EVTs and HTR8 express IL-25 and IL-17RB. The expressions increase in third-trimester during normal pregnancy. In PE, both of IL-25 and IL-17RB expressions decrease (p< 0.05). IL-25 for 24 h promoted HTR8 proliferation and invasion (p< 0.05). The effect was abolished in the presence of IL-25Ab (p< 0.05). Conclusion: This study demonstrates that IL-25 may contribute to promote trophoblast invasion and proliferation and abnormal decline of IL-25 might be associated with PE.http://dx.doi.org/10.1080/10641955.2021.1950177il-25/trophoblast/proliferation/ invasion/ pre-eclampsia |
| spellingShingle | Siyu Liu Yi Sun Yao Tang Rong Hu Qiongjie Zhou Xiaotian Li IL-25 promotes trophoblast proliferation and invasion via binding with IL-17RB and associated with PE Hypertension in Pregnancy il-25/trophoblast/proliferation/ invasion/ pre-eclampsia |
| title | IL-25 promotes trophoblast proliferation and invasion via binding with IL-17RB and associated with PE |
| title_full | IL-25 promotes trophoblast proliferation and invasion via binding with IL-17RB and associated with PE |
| title_fullStr | IL-25 promotes trophoblast proliferation and invasion via binding with IL-17RB and associated with PE |
| title_full_unstemmed | IL-25 promotes trophoblast proliferation and invasion via binding with IL-17RB and associated with PE |
| title_short | IL-25 promotes trophoblast proliferation and invasion via binding with IL-17RB and associated with PE |
| title_sort | il 25 promotes trophoblast proliferation and invasion via binding with il 17rb and associated with pe |
| topic | il-25/trophoblast/proliferation/ invasion/ pre-eclampsia |
| url | http://dx.doi.org/10.1080/10641955.2021.1950177 |
| work_keys_str_mv | AT siyuliu il25promotestrophoblastproliferationandinvasionviabindingwithil17rbandassociatedwithpe AT yisun il25promotestrophoblastproliferationandinvasionviabindingwithil17rbandassociatedwithpe AT yaotang il25promotestrophoblastproliferationandinvasionviabindingwithil17rbandassociatedwithpe AT ronghu il25promotestrophoblastproliferationandinvasionviabindingwithil17rbandassociatedwithpe AT qiongjiezhou il25promotestrophoblastproliferationandinvasionviabindingwithil17rbandassociatedwithpe AT xiaotianli il25promotestrophoblastproliferationandinvasionviabindingwithil17rbandassociatedwithpe |