Sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically-important nitro compounds
Abstract In this study, highly fluorescent sulfur and nitrogen co-doped carbon quantum dots (SN-CQDs) were synthesized by a simple one-pot hydrothermal method using thiosemicarbazide and citric acid as starting materials. Various spectroscopic and microscopic techniques were applied to characterize...
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-32494-8 |
| _version_ | 1797850030060601344 |
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| author | Galal Magdy Shaimaa Ebrahim Fathalla Belal Ramadan A. El-Domany Ahmed M. Abdel-Megied |
| author_facet | Galal Magdy Shaimaa Ebrahim Fathalla Belal Ramadan A. El-Domany Ahmed M. Abdel-Megied |
| author_sort | Galal Magdy |
| collection | DOAJ |
| description | Abstract In this study, highly fluorescent sulfur and nitrogen co-doped carbon quantum dots (SN-CQDs) were synthesized by a simple one-pot hydrothermal method using thiosemicarbazide and citric acid as starting materials. Various spectroscopic and microscopic techniques were applied to characterize the prepared SN-CQDs. The synthesized SN-CQDs’ maximum fluorescence emission was obtained at 430 nm after excitation at 360 nm. Rifampicin (RFP), tinidazole (TNZ), ornidazole (ONZ), and metronidazole (MNZ) all quantitatively and selectively quenched the SN-CQDs’ native fluorescence, which was the base-for their-spectrofluorimetric estimation without the need for any tedious pre-treatment steps or high-cost instrumentation. SN-CQDs demonstrated a “turn-off” fluorescence response to RFP, TNZ, ONZ, and MNZ over the ranges of 1.0–30.0, 10.0–200.0, 6.0–200.0, and 5.0–100.0 μM with detection limits of 0.31, 1.76, 0.57, and 0.75 μM and quantitation limits of 0.93, 5.32, 1.74, and 2.28 μM respectively. The suggested method was successfully used to determine the investigated drugs in their commercial dosage forms. The method was further extended to their determination in spiked human plasma samples, with satisfactory mean % recoveries (99.44–100.29) and low % RSD values (< 4.52). The mechanism of fluorescence quenching was studied and discussed. The suggested method was validated in accordance with ICH recommendations. |
| first_indexed | 2024-04-09T18:54:50Z |
| format | Article |
| id | doaj.art-523e8e8a41164dab9dfb94cb6c58811e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T18:54:50Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-523e8e8a41164dab9dfb94cb6c58811e2023-04-09T11:15:55ZengNature PortfolioScientific Reports2045-23222023-04-0113111910.1038/s41598-023-32494-8Sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically-important nitro compoundsGalal Magdy0Shaimaa Ebrahim1Fathalla Belal2Ramadan A. El-Domany3Ahmed M. Abdel-Megied4Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Kafrelsheikh UniversityPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Kafrelsheikh UniversityPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura UniversityMicrobiology and Immunology Department, Faculty of Pharmacy, Kafrelsheikh UniversityPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Kafrelsheikh UniversityAbstract In this study, highly fluorescent sulfur and nitrogen co-doped carbon quantum dots (SN-CQDs) were synthesized by a simple one-pot hydrothermal method using thiosemicarbazide and citric acid as starting materials. Various spectroscopic and microscopic techniques were applied to characterize the prepared SN-CQDs. The synthesized SN-CQDs’ maximum fluorescence emission was obtained at 430 nm after excitation at 360 nm. Rifampicin (RFP), tinidazole (TNZ), ornidazole (ONZ), and metronidazole (MNZ) all quantitatively and selectively quenched the SN-CQDs’ native fluorescence, which was the base-for their-spectrofluorimetric estimation without the need for any tedious pre-treatment steps or high-cost instrumentation. SN-CQDs demonstrated a “turn-off” fluorescence response to RFP, TNZ, ONZ, and MNZ over the ranges of 1.0–30.0, 10.0–200.0, 6.0–200.0, and 5.0–100.0 μM with detection limits of 0.31, 1.76, 0.57, and 0.75 μM and quantitation limits of 0.93, 5.32, 1.74, and 2.28 μM respectively. The suggested method was successfully used to determine the investigated drugs in their commercial dosage forms. The method was further extended to their determination in spiked human plasma samples, with satisfactory mean % recoveries (99.44–100.29) and low % RSD values (< 4.52). The mechanism of fluorescence quenching was studied and discussed. The suggested method was validated in accordance with ICH recommendations.https://doi.org/10.1038/s41598-023-32494-8 |
| spellingShingle | Galal Magdy Shaimaa Ebrahim Fathalla Belal Ramadan A. El-Domany Ahmed M. Abdel-Megied Sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically-important nitro compounds Scientific Reports |
| title | Sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically-important nitro compounds |
| title_full | Sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically-important nitro compounds |
| title_fullStr | Sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically-important nitro compounds |
| title_full_unstemmed | Sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically-important nitro compounds |
| title_short | Sulfur and nitrogen co-doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically-important nitro compounds |
| title_sort | sulfur and nitrogen co doped carbon quantum dots as fluorescent probes for the determination of some pharmaceutically important nitro compounds |
| url | https://doi.org/10.1038/s41598-023-32494-8 |
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