Mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co-culture system
Ultraviolet radiation induces skin damage, inflammation, and aging. To explore the protective effects of blueberry extract (BE) against ultraviolet-C (UV-C) radiation, we analysed human skin keratinocyte (HaCaT) and human foreskin fibroblast (HFF) cells alone or in a co-culture, treated or not with...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-01-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618306108 |
| _version_ | 1819044286737416192 |
|---|---|
| author | Huailing Wang Jie Liu Daorui Pang Tong Li Rui Hai Liu |
| author_facet | Huailing Wang Jie Liu Daorui Pang Tong Li Rui Hai Liu |
| author_sort | Huailing Wang |
| collection | DOAJ |
| description | Ultraviolet radiation induces skin damage, inflammation, and aging. To explore the protective effects of blueberry extract (BE) against ultraviolet-C (UV-C) radiation, we analysed human skin keratinocyte (HaCaT) and human foreskin fibroblast (HFF) cells alone or in a co-culture, treated or not with BE, after exposure to UV-C. Surprisingly, MMP-1 was significantly up-regulated in HFF cells and in the co-cultured cell group in response to UV-C irradiation (8 mJ/cm2), but not in HaCaT cells. As expected, BE had a significantly protective effects; it decreased DNA fragmentation and inhibited MMP-1 and inflammatory factors expression in response to UV-C radiation. In addition, BE reduced the accumulation of proteins (MMP-1, c-fos, and c-jnu) in the MAPK (mitogen-activated protein kinase) pathway after UV-C radiation. Taken together, these results showed that BE exerted a protective effect against UV-C irradiation, and modulated MMP-1 production through MAPK signalling by direct and indirect pathways in HaCaT and HFF cells. |
| first_indexed | 2024-12-21T10:10:16Z |
| format | Article |
| id | doaj.art-52424ab665244b41bd5b6312b511564b |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-21T10:10:16Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-52424ab665244b41bd5b6312b511564b2022-12-21T19:07:43ZengElsevierJournal of Functional Foods1756-46462019-01-0152603610Mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co-culture systemHuailing Wang0Jie Liu1Daorui Pang2Tong Li3Rui Hai Liu4School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China; Corresponding authors.Department of Allergy, The Third Affiliated Hospital of Shenzhen University, Shenzhen 518020, ChinaSchool of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, ChinaDepartment of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USADepartment of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USA; Corresponding authors.Ultraviolet radiation induces skin damage, inflammation, and aging. To explore the protective effects of blueberry extract (BE) against ultraviolet-C (UV-C) radiation, we analysed human skin keratinocyte (HaCaT) and human foreskin fibroblast (HFF) cells alone or in a co-culture, treated or not with BE, after exposure to UV-C. Surprisingly, MMP-1 was significantly up-regulated in HFF cells and in the co-cultured cell group in response to UV-C irradiation (8 mJ/cm2), but not in HaCaT cells. As expected, BE had a significantly protective effects; it decreased DNA fragmentation and inhibited MMP-1 and inflammatory factors expression in response to UV-C radiation. In addition, BE reduced the accumulation of proteins (MMP-1, c-fos, and c-jnu) in the MAPK (mitogen-activated protein kinase) pathway after UV-C radiation. Taken together, these results showed that BE exerted a protective effect against UV-C irradiation, and modulated MMP-1 production through MAPK signalling by direct and indirect pathways in HaCaT and HFF cells.http://www.sciencedirect.com/science/article/pii/S1756464618306108Blueberry extractsUVCCo-culture modelsMAPK signalsAnti-ultraviolet |
| spellingShingle | Huailing Wang Jie Liu Daorui Pang Tong Li Rui Hai Liu Mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co-culture system Journal of Functional Foods Blueberry extracts UVC Co-culture models MAPK signals Anti-ultraviolet |
| title | Mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co-culture system |
| title_full | Mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co-culture system |
| title_fullStr | Mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co-culture system |
| title_full_unstemmed | Mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co-culture system |
| title_short | Mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co-culture system |
| title_sort | mechanisms underlying the protective effects of blueberry extract against ultraviolet radiation in a skin cell co culture system |
| topic | Blueberry extracts UVC Co-culture models MAPK signals Anti-ultraviolet |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618306108 |
| work_keys_str_mv | AT huailingwang mechanismsunderlyingtheprotectiveeffectsofblueberryextractagainstultravioletradiationinaskincellcoculturesystem AT jieliu mechanismsunderlyingtheprotectiveeffectsofblueberryextractagainstultravioletradiationinaskincellcoculturesystem AT daoruipang mechanismsunderlyingtheprotectiveeffectsofblueberryextractagainstultravioletradiationinaskincellcoculturesystem AT tongli mechanismsunderlyingtheprotectiveeffectsofblueberryextractagainstultravioletradiationinaskincellcoculturesystem AT ruihailiu mechanismsunderlyingtheprotectiveeffectsofblueberryextractagainstultravioletradiationinaskincellcoculturesystem |