Essential oil of Croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin-induced diabetes mellitus in rats
Croton zehntneri is an aromatic shrub, native to Northeast Brazil, where it is used in folk medicine. The essential oil of C. zehntneri (EOCz) has shown potent antioxidant and anti-inflammatory activities. Our aim was to investigate the beneficial effect of EOCz on electrophysiological changes in do...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031323000398 |
| _version_ | 1827950188278317056 |
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| author | Francisco Walber Ferreira-da-Silva Kerly Shamyra da Silva-Alves Andrelina Noronha Coelho-de-Souza José Henrique Leal-Cardoso |
| author_facet | Francisco Walber Ferreira-da-Silva Kerly Shamyra da Silva-Alves Andrelina Noronha Coelho-de-Souza José Henrique Leal-Cardoso |
| author_sort | Francisco Walber Ferreira-da-Silva |
| collection | DOAJ |
| description | Croton zehntneri is an aromatic shrub, native to Northeast Brazil, where it is used in folk medicine. The essential oil of C. zehntneri (EOCz) has shown potent antioxidant and anti-inflammatory activities. Our aim was to investigate the beneficial effect of EOCz on electrophysiological changes in dorsal root ganglia (DRG) of rats with streptozotocin-induced diabetes mellitus (DM). DRG excitability and passive and active membrane properties were studied in control and DM-induced rats. Intracellular recording in current-clamp mode with sharp microelectrode was used. For Na+ channel investigation, patch-clamp recording in whole-cell mode was used. DM caused depolarizations (from -60.6 to -55.0 mV) of resting membrane potential without alteration of cell membrane input resistance. Also, it showed a tendency towards a decrease in rheobase and a tendency for increase in excitability, which was confirmed by increase in Na+ current (from -113.6 to -178.3 pF/pA). EOCz did not reverse the hyperglycemia of DM. Regardless of that, upon oral treatment, EOCz prevented neuronal alterations. EOCz was demonstrated to have low toxicity. The preventive effects of EOCz on neuronal changes coupled with its low toxicity make EOCz a potential candidate for a type of treatment of diabetic neuropathy complementary to therapy with hypoglycemic agents. |
| first_indexed | 2024-04-09T13:21:55Z |
| format | Article |
| id | doaj.art-5242945706f64c84869ca12b7cb8434d |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-04-09T13:21:55Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-5242945706f64c84869ca12b7cb8434d2023-05-11T04:24:56ZengElsevierPhytomedicine Plus2667-03132023-05-0132100443Essential oil of Croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin-induced diabetes mellitus in ratsFrancisco Walber Ferreira-da-Silva0Kerly Shamyra da Silva-Alves1Andrelina Noronha Coelho-de-Souza2José Henrique Leal-Cardoso3Laboratory of Electrophysiology, Superior Institute of Biomedical Science, State University of Ceará, 1700 Silas Munguba Av., Campus of Itaperi, CEP, Fortaleza, Ceará, 60740-903, Brazil; Center of Exact Sciences and Technology, Civil Engineering Department, State University of Vale do Acaraú, 317 Dr. Guarany Av., Campus of Cidao, Sobral, Ceará, 62010-305, BrazilLaboratory of Electrophysiology, Superior Institute of Biomedical Science, State University of Ceará, 1700 Silas Munguba Av., Campus of Itaperi, CEP, Fortaleza, Ceará, 60740-903, BrazilLaboratory of Experimental Physiology, Superior Institute of Biomedical Science, State University of Ceará, 1700 Silas Munguba Av., Campus of Itaperi, Fortaleza, Ceará, 60740-903, BrazilLaboratory of Electrophysiology, Superior Institute of Biomedical Science, State University of Ceará, 1700 Silas Munguba Av., Campus of Itaperi, CEP, Fortaleza, Ceará, 60740-903, Brazil; Corresponding author.Croton zehntneri is an aromatic shrub, native to Northeast Brazil, where it is used in folk medicine. The essential oil of C. zehntneri (EOCz) has shown potent antioxidant and anti-inflammatory activities. Our aim was to investigate the beneficial effect of EOCz on electrophysiological changes in dorsal root ganglia (DRG) of rats with streptozotocin-induced diabetes mellitus (DM). DRG excitability and passive and active membrane properties were studied in control and DM-induced rats. Intracellular recording in current-clamp mode with sharp microelectrode was used. For Na+ channel investigation, patch-clamp recording in whole-cell mode was used. DM caused depolarizations (from -60.6 to -55.0 mV) of resting membrane potential without alteration of cell membrane input resistance. Also, it showed a tendency towards a decrease in rheobase and a tendency for increase in excitability, which was confirmed by increase in Na+ current (from -113.6 to -178.3 pF/pA). EOCz did not reverse the hyperglycemia of DM. Regardless of that, upon oral treatment, EOCz prevented neuronal alterations. EOCz was demonstrated to have low toxicity. The preventive effects of EOCz on neuronal changes coupled with its low toxicity make EOCz a potential candidate for a type of treatment of diabetic neuropathy complementary to therapy with hypoglycemic agents.http://www.sciencedirect.com/science/article/pii/S2667031323000398Croton zehntneriEssential oilPeripheral diabetic neuropathyDorsal root gangliaResting membrane potentialSodium current |
| spellingShingle | Francisco Walber Ferreira-da-Silva Kerly Shamyra da Silva-Alves Andrelina Noronha Coelho-de-Souza José Henrique Leal-Cardoso Essential oil of Croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin-induced diabetes mellitus in rats Phytomedicine Plus Croton zehntneri Essential oil Peripheral diabetic neuropathy Dorsal root ganglia Resting membrane potential Sodium current |
| title | Essential oil of Croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin-induced diabetes mellitus in rats |
| title_full | Essential oil of Croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin-induced diabetes mellitus in rats |
| title_fullStr | Essential oil of Croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin-induced diabetes mellitus in rats |
| title_full_unstemmed | Essential oil of Croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin-induced diabetes mellitus in rats |
| title_short | Essential oil of Croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin-induced diabetes mellitus in rats |
| title_sort | essential oil of croton zehntneri prevents electrophysiological alterations in dorsal root ganglia of streptozotocin induced diabetes mellitus in rats |
| topic | Croton zehntneri Essential oil Peripheral diabetic neuropathy Dorsal root ganglia Resting membrane potential Sodium current |
| url | http://www.sciencedirect.com/science/article/pii/S2667031323000398 |
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