Morphometrical Identification and Phylogenetic Analysis of Rhinonyssidae (Acari: Mesostigmata) Parasitizing Avian Hosts: New Molecular Data
Members of the family Rhinonyssidae are tiny hematophagous endoparasitic mites that inhabit the nasal cavities of birds and can cause trauma to their hosts. Traditionally, identifying species in this group has relied on observing their morphometrical characteristics. Nevertheless, determining specie...
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MDPI AG
2023-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/7/1783 |
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| author | Susana A. Sánchez-Carrión Ivan Dimov Francisco J. Márquez Jiménez Manuel de Rojas Álvarez |
| author_facet | Susana A. Sánchez-Carrión Ivan Dimov Francisco J. Márquez Jiménez Manuel de Rojas Álvarez |
| author_sort | Susana A. Sánchez-Carrión |
| collection | DOAJ |
| description | Members of the family Rhinonyssidae are tiny hematophagous endoparasitic mites that inhabit the nasal cavities of birds and can cause trauma to their hosts. Traditionally, identifying species in this group has relied on observing their morphometrical characteristics. Nevertheless, determining species within this particular group has become more challenging due to the rising number of newly discovered species. Moreover, the morphometrical traits vary depending on the specific genus or group of species being studied. In this study, the complete internal transcribed spacer ITS1, 5.8S rDNA, and ITS2 regions of the ribosomal DNA from eighteen species of rhinonyssid mites belonging to four genera were sequenced to assess the utility of this genomic region in resolving taxonomic questions in this group and to estimate the phylogenetic relationships among the species. Mites were collected by dissecting the nasal cavities of birds under a stereomicroscope. Specimens used for morphometrical analyses were cleared in 85% lactic acid for 1–48 h and mounted in Hoyer’s medium. Other specimens were preserved at −20 °C for molecular studies. From the data obtained in this study, it can be concluded that a thorough review and an accurate morphometrical identification and determination of the discriminatory traits are needed in this group of mites. Moreover, although the ITS1–5.8S–ITS2 fragment solves different taxonomic and phylogenetic problems at the species level, it would be necessary to test new molecular markers, or even a combination of nuclear and mitochondrial markers or different domains of the nuclear 28S rDNA, to discover a reliable taxonomic situation for rhinonyssids. |
| first_indexed | 2024-03-11T00:48:43Z |
| format | Article |
| id | doaj.art-5243636350c342bd8335022a967e8924 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T00:48:43Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5243636350c342bd8335022a967e89242023-11-18T20:36:23ZengMDPI AGMicroorganisms2076-26072023-07-01117178310.3390/microorganisms11071783Morphometrical Identification and Phylogenetic Analysis of Rhinonyssidae (Acari: Mesostigmata) Parasitizing Avian Hosts: New Molecular DataSusana A. Sánchez-Carrión0Ivan Dimov1Francisco J. Márquez Jiménez2Manuel de Rojas Álvarez3Departament of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, SpainDepartment of Clinical Anatomy and Operative Surgery Named after Professor M.G. Prives, Pavlov First Saint Petersburg State Medical University, L’va Tolstogo Str. 6-8, Saint Petersburg 197022, RussiaDepartment of Animal Biology, Vegetal Biology and Ecology, Faculty of Experimental Sciences, Universidad de Jaén, 23071 Jaén, SpainDepartament of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, SpainMembers of the family Rhinonyssidae are tiny hematophagous endoparasitic mites that inhabit the nasal cavities of birds and can cause trauma to their hosts. Traditionally, identifying species in this group has relied on observing their morphometrical characteristics. Nevertheless, determining species within this particular group has become more challenging due to the rising number of newly discovered species. Moreover, the morphometrical traits vary depending on the specific genus or group of species being studied. In this study, the complete internal transcribed spacer ITS1, 5.8S rDNA, and ITS2 regions of the ribosomal DNA from eighteen species of rhinonyssid mites belonging to four genera were sequenced to assess the utility of this genomic region in resolving taxonomic questions in this group and to estimate the phylogenetic relationships among the species. Mites were collected by dissecting the nasal cavities of birds under a stereomicroscope. Specimens used for morphometrical analyses were cleared in 85% lactic acid for 1–48 h and mounted in Hoyer’s medium. Other specimens were preserved at −20 °C for molecular studies. From the data obtained in this study, it can be concluded that a thorough review and an accurate morphometrical identification and determination of the discriminatory traits are needed in this group of mites. Moreover, although the ITS1–5.8S–ITS2 fragment solves different taxonomic and phylogenetic problems at the species level, it would be necessary to test new molecular markers, or even a combination of nuclear and mitochondrial markers or different domains of the nuclear 28S rDNA, to discover a reliable taxonomic situation for rhinonyssids.https://www.mdpi.com/2076-2607/11/7/1783mitesmolecular systematicsRhinonyssidaemorphometricalphylogenetic |
| spellingShingle | Susana A. Sánchez-Carrión Ivan Dimov Francisco J. Márquez Jiménez Manuel de Rojas Álvarez Morphometrical Identification and Phylogenetic Analysis of Rhinonyssidae (Acari: Mesostigmata) Parasitizing Avian Hosts: New Molecular Data Microorganisms mites molecular systematics Rhinonyssidae morphometrical phylogenetic |
| title | Morphometrical Identification and Phylogenetic Analysis of Rhinonyssidae (Acari: Mesostigmata) Parasitizing Avian Hosts: New Molecular Data |
| title_full | Morphometrical Identification and Phylogenetic Analysis of Rhinonyssidae (Acari: Mesostigmata) Parasitizing Avian Hosts: New Molecular Data |
| title_fullStr | Morphometrical Identification and Phylogenetic Analysis of Rhinonyssidae (Acari: Mesostigmata) Parasitizing Avian Hosts: New Molecular Data |
| title_full_unstemmed | Morphometrical Identification and Phylogenetic Analysis of Rhinonyssidae (Acari: Mesostigmata) Parasitizing Avian Hosts: New Molecular Data |
| title_short | Morphometrical Identification and Phylogenetic Analysis of Rhinonyssidae (Acari: Mesostigmata) Parasitizing Avian Hosts: New Molecular Data |
| title_sort | morphometrical identification and phylogenetic analysis of rhinonyssidae acari mesostigmata parasitizing avian hosts new molecular data |
| topic | mites molecular systematics Rhinonyssidae morphometrical phylogenetic |
| url | https://www.mdpi.com/2076-2607/11/7/1783 |
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