Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.
Among the various posttranslational modification reactions, glycosylation is the most common, and nearly 50% of all known proteins are thought to be glycosylated. In fact, changes in glycosylation readily occur in carcinogenesis, invasion and metastasis. This report investigated the modification of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3688732?pdf=render |
| _version_ | 1818513084042444800 |
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| author | Zhaohai Zhang Jie Sun Lihong Hao Chunqing Liu Hongye Ma Li Jia |
| author_facet | Zhaohai Zhang Jie Sun Lihong Hao Chunqing Liu Hongye Ma Li Jia |
| author_sort | Zhaohai Zhang |
| collection | DOAJ |
| description | Among the various posttranslational modification reactions, glycosylation is the most common, and nearly 50% of all known proteins are thought to be glycosylated. In fact, changes in glycosylation readily occur in carcinogenesis, invasion and metastasis. This report investigated the modification of glycosylation mediated the invasive properties of Hca-F and Hca-P murine hepatocarcinoma cell lines, which have high, low metastatic potential in the lymph nodes, respectively. Analysis revealed that the N-glycan composition profiling, expression of glycogenes and lectin binding profiling were different in Hca-F cells, as compared to those in Hca-P cells. Further analysis of the N-glycan regulation by tunicamycin (TM) application or PNGase F treatment in Hca-F cells showed partial inhibition of N-glycan glycosylation and decreased invasion both in vitro and in vivo. We targeted glycogene ST6GAL1, which was expressed differently in Hca-F and Hca-P cells, and regulated the expression of ST6GAL1. The altered levels of ST6GAL1 were also responsible for changed invasive properties of Hca-F and Hca-P cells both in vitro and in vivo. These findings indicate a role for glycosylation modification as a mediator of tumor lymphatic metastasis, with its altered expression causing an invasive ability differentially. |
| first_indexed | 2024-12-10T23:56:06Z |
| format | Article |
| id | doaj.art-5243f83f4da745e38cb3e43a48dc0d95 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T23:56:06Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5243f83f4da745e38cb3e43a48dc0d952022-12-22T01:28:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6521810.1371/journal.pone.0065218Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes.Zhaohai ZhangJie SunLihong HaoChunqing LiuHongye MaLi JiaAmong the various posttranslational modification reactions, glycosylation is the most common, and nearly 50% of all known proteins are thought to be glycosylated. In fact, changes in glycosylation readily occur in carcinogenesis, invasion and metastasis. This report investigated the modification of glycosylation mediated the invasive properties of Hca-F and Hca-P murine hepatocarcinoma cell lines, which have high, low metastatic potential in the lymph nodes, respectively. Analysis revealed that the N-glycan composition profiling, expression of glycogenes and lectin binding profiling were different in Hca-F cells, as compared to those in Hca-P cells. Further analysis of the N-glycan regulation by tunicamycin (TM) application or PNGase F treatment in Hca-F cells showed partial inhibition of N-glycan glycosylation and decreased invasion both in vitro and in vivo. We targeted glycogene ST6GAL1, which was expressed differently in Hca-F and Hca-P cells, and regulated the expression of ST6GAL1. The altered levels of ST6GAL1 were also responsible for changed invasive properties of Hca-F and Hca-P cells both in vitro and in vivo. These findings indicate a role for glycosylation modification as a mediator of tumor lymphatic metastasis, with its altered expression causing an invasive ability differentially.http://europepmc.org/articles/PMC3688732?pdf=render |
| spellingShingle | Zhaohai Zhang Jie Sun Lihong Hao Chunqing Liu Hongye Ma Li Jia Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes. PLoS ONE |
| title | Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes. |
| title_full | Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes. |
| title_fullStr | Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes. |
| title_full_unstemmed | Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes. |
| title_short | Modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes. |
| title_sort | modification of glycosylation mediates the invasive properties of murine hepatocarcinoma cell lines to lymph nodes |
| url | http://europepmc.org/articles/PMC3688732?pdf=render |
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