Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants
Inflammatory bowel diseases (IBDs) are autoimmune disorders of the gut. It is increasingly clear that voluntary exercise (VE) may exert protection against IBDs, but the exact background mechanism needs to be elucidated. In the present study, we aimed to investigate the possible role of NETosis and t...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/8/1531 |
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| author | Nikoletta Almási Szilvia Török Amin Al-awar Médea Veszelka László Király Denise Börzsei Renáta Szabó Csaba Varga |
| author_facet | Nikoletta Almási Szilvia Török Amin Al-awar Médea Veszelka László Király Denise Börzsei Renáta Szabó Csaba Varga |
| author_sort | Nikoletta Almási |
| collection | DOAJ |
| description | Inflammatory bowel diseases (IBDs) are autoimmune disorders of the gut. It is increasingly clear that voluntary exercise (VE) may exert protection against IBDs, but the exact background mechanism needs to be elucidated. In the present study, we aimed to investigate the possible role of NETosis and the antioxidant peroxiredoxin (Prdx) enzyme family in VE-induced protection. Wistar Han rats were randomly divided into two groups: sedentary (SED) and VE. After the 6-week voluntary wheel running, animals were treated with 2,4,6-trinitrobenzene sulphonic acid (TNBS) as a model of colitis. Here, we found that VE significantly decreased inflammation and ulceration of the colon in the VE TNBS group compared with SED TNBS. We also found that VE significantly decreased the expression of protein arginine deiminase 4 (PAD4) and myeloperoxidase (MPO), and markedly reduced citrullinated histone H3 (citH3) compared with SED TNBS. Furthermore, VE caused a significant increase in the levels of Prdx6 in the control and TNBS groups. Taken together, we found that a prior 6-week VE effectively reduces inflammation in TNBS-induced colitis, and we suggest that the protective effect of VE may be mediated via the inhibition of NETosis and upregulation of Prdx6 antioxidant. |
| first_indexed | 2024-03-11T00:10:26Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T00:10:26Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-524748c69dea478c95dca60f6d1fd5b82023-11-19T00:01:17ZengMDPI AGAntioxidants2076-39212023-07-01128153110.3390/antiox12081531Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx AntioxidantsNikoletta Almási0Szilvia Török1Amin Al-awar2Médea Veszelka3László Király4Denise Börzsei5Renáta Szabó6Csaba Varga7Department of Physiology, Anatomy and Neuroscience, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, H-6726 Szeged, HungaryZala-Cereália Kft, H-8790 Zalaszentgrót-Tüskeszentpéter, HungaryDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, University of Szeged, H-6726 Szeged, HungaryInflammatory bowel diseases (IBDs) are autoimmune disorders of the gut. It is increasingly clear that voluntary exercise (VE) may exert protection against IBDs, but the exact background mechanism needs to be elucidated. In the present study, we aimed to investigate the possible role of NETosis and the antioxidant peroxiredoxin (Prdx) enzyme family in VE-induced protection. Wistar Han rats were randomly divided into two groups: sedentary (SED) and VE. After the 6-week voluntary wheel running, animals were treated with 2,4,6-trinitrobenzene sulphonic acid (TNBS) as a model of colitis. Here, we found that VE significantly decreased inflammation and ulceration of the colon in the VE TNBS group compared with SED TNBS. We also found that VE significantly decreased the expression of protein arginine deiminase 4 (PAD4) and myeloperoxidase (MPO), and markedly reduced citrullinated histone H3 (citH3) compared with SED TNBS. Furthermore, VE caused a significant increase in the levels of Prdx6 in the control and TNBS groups. Taken together, we found that a prior 6-week VE effectively reduces inflammation in TNBS-induced colitis, and we suggest that the protective effect of VE may be mediated via the inhibition of NETosis and upregulation of Prdx6 antioxidant.https://www.mdpi.com/2076-3921/12/8/1531voluntary exerciseinflammationperoxiredoxinIBDS |
| spellingShingle | Nikoletta Almási Szilvia Török Amin Al-awar Médea Veszelka László Király Denise Börzsei Renáta Szabó Csaba Varga Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants Antioxidants voluntary exercise inflammation peroxiredoxin IBDS |
| title | Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants |
| title_full | Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants |
| title_fullStr | Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants |
| title_full_unstemmed | Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants |
| title_short | Voluntary Exercise-Mediated Protection in TNBS-Induced Rat Colitis: The Involvement of NETosis and Prdx Antioxidants |
| title_sort | voluntary exercise mediated protection in tnbs induced rat colitis the involvement of netosis and prdx antioxidants |
| topic | voluntary exercise inflammation peroxiredoxin IBDS |
| url | https://www.mdpi.com/2076-3921/12/8/1531 |
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