Optimization of Culture Parameters for Improved Production of Bioactive Metabolite by Endophytic Geosmithia pallida (KU693285) Isolated from Brucea mollis Wall ex. Kurz, An Endangered Medicinal Plant
The present study envisaged to optimise the culture parameters for improved bioactive secondary metabolite production by Geosmithia pallida (KU693285), a potent endophytic fungi, isolated from endangered medicinal plant Brucea mollis Wall ex. Kurz. G. pallida was isolated from healthy, surface st...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2018-09-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/optimization-of-culture-parameters-for-improved-production-of-bioactive-metabolite-by-endophytic-geosmithia-pallida-ku693285-isolated-from-brucea-mollis-wall-ex-kurz-an-endangered-medicinal-plant/ |
| _version_ | 1818691038237163520 |
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| author | Deepanwita Deka Dhruva Kumar Jha |
| author_facet | Deepanwita Deka Dhruva Kumar Jha |
| author_sort | Deepanwita Deka |
| collection | DOAJ |
| description | The present study envisaged to optimise the culture parameters for improved bioactive
secondary metabolite production by Geosmithia pallida (KU693285), a potent endophytic
fungi, isolated from endangered medicinal plant Brucea mollis Wall ex. Kurz. G. pallida was
isolated from healthy, surface sterilized leaf, bark and root tissues of B. mollis. Sporulation was
induced in sterile G. pallida by growing it on modified PDA media. Identification of G. Pallida
was confirmed by ITS r-DNA sequence analysis. The culture parameters were optimized for
enhanced production of metabolites. Maximum metabolite production was observed at 25°C
incubation temperature and 6.5 pH on 28th day of inoculation, when lactose and yeast extract
were added in the basal medium respectively as carbon and nitrogen source along with 3.5 g/l
NaCl. The inhibition zone showed by G. pallida was significantly higher during the optimum
culture conditions than that of the control (basal media). From the present study, it is concluded
that for the maximum production of bioactive metabolite the culture parameters need to be
optimized. These results would steer the authors to carry out further studies on purification,
characterization, and identification of bioactive metabolites produced by G. pallida (KU693285)
for pharmaceutical as well as agricultural applications. |
| first_indexed | 2024-12-17T12:35:32Z |
| format | Article |
| id | doaj.art-524960c887a0464caed4e391297fa1d0 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-17T12:35:32Z |
| publishDate | 2018-09-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-524960c887a0464caed4e391297fa1d02022-12-21T21:48:19ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2018-09-011231205121310.22207/JPAM.12.3.21Optimization of Culture Parameters for Improved Production of Bioactive Metabolite by Endophytic Geosmithia pallida (KU693285) Isolated from Brucea mollis Wall ex. Kurz, An Endangered Medicinal PlantDeepanwita Deka0Dhruva Kumar Jha1Microbial Ecology Laboratory, Department of Botany, Gauhati University, Guwahati - 781 014, Assam, India.Microbial Ecology Laboratory, Department of Botany, Gauhati University, Guwahati - 781 014, Assam, India.The present study envisaged to optimise the culture parameters for improved bioactive secondary metabolite production by Geosmithia pallida (KU693285), a potent endophytic fungi, isolated from endangered medicinal plant Brucea mollis Wall ex. Kurz. G. pallida was isolated from healthy, surface sterilized leaf, bark and root tissues of B. mollis. Sporulation was induced in sterile G. pallida by growing it on modified PDA media. Identification of G. Pallida was confirmed by ITS r-DNA sequence analysis. The culture parameters were optimized for enhanced production of metabolites. Maximum metabolite production was observed at 25°C incubation temperature and 6.5 pH on 28th day of inoculation, when lactose and yeast extract were added in the basal medium respectively as carbon and nitrogen source along with 3.5 g/l NaCl. The inhibition zone showed by G. pallida was significantly higher during the optimum culture conditions than that of the control (basal media). From the present study, it is concluded that for the maximum production of bioactive metabolite the culture parameters need to be optimized. These results would steer the authors to carry out further studies on purification, characterization, and identification of bioactive metabolites produced by G. pallida (KU693285) for pharmaceutical as well as agricultural applications.https://microbiologyjournal.org/optimization-of-culture-parameters-for-improved-production-of-bioactive-metabolite-by-endophytic-geosmithia-pallida-ku693285-isolated-from-brucea-mollis-wall-ex-kurz-an-endangered-medicinal-plant/endophytic fungigeosmithia pallida (ku693285)bioactive secondary metaboliteoptimizationcultural parameters |
| spellingShingle | Deepanwita Deka Dhruva Kumar Jha Optimization of Culture Parameters for Improved Production of Bioactive Metabolite by Endophytic Geosmithia pallida (KU693285) Isolated from Brucea mollis Wall ex. Kurz, An Endangered Medicinal Plant Journal of Pure and Applied Microbiology endophytic fungi geosmithia pallida (ku693285) bioactive secondary metabolite optimization cultural parameters |
| title | Optimization of Culture Parameters for Improved Production of Bioactive Metabolite by Endophytic Geosmithia pallida (KU693285) Isolated from Brucea mollis Wall ex. Kurz, An Endangered Medicinal Plant |
| title_full | Optimization of Culture Parameters for Improved Production of Bioactive Metabolite by Endophytic Geosmithia pallida (KU693285) Isolated from Brucea mollis Wall ex. Kurz, An Endangered Medicinal Plant |
| title_fullStr | Optimization of Culture Parameters for Improved Production of Bioactive Metabolite by Endophytic Geosmithia pallida (KU693285) Isolated from Brucea mollis Wall ex. Kurz, An Endangered Medicinal Plant |
| title_full_unstemmed | Optimization of Culture Parameters for Improved Production of Bioactive Metabolite by Endophytic Geosmithia pallida (KU693285) Isolated from Brucea mollis Wall ex. Kurz, An Endangered Medicinal Plant |
| title_short | Optimization of Culture Parameters for Improved Production of Bioactive Metabolite by Endophytic Geosmithia pallida (KU693285) Isolated from Brucea mollis Wall ex. Kurz, An Endangered Medicinal Plant |
| title_sort | optimization of culture parameters for improved production of bioactive metabolite by endophytic geosmithia pallida ku693285 isolated from brucea mollis wall ex kurz an endangered medicinal plant |
| topic | endophytic fungi geosmithia pallida (ku693285) bioactive secondary metabolite optimization cultural parameters |
| url | https://microbiologyjournal.org/optimization-of-culture-parameters-for-improved-production-of-bioactive-metabolite-by-endophytic-geosmithia-pallida-ku693285-isolated-from-brucea-mollis-wall-ex-kurz-an-endangered-medicinal-plant/ |
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