Effect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening
Objective: To prepare different polarities leave crude extracts of Juniperus excels (J. excels) and to determine their phytochemical screening, antioxidant, antimicrobial and cytotoxic activities. Methods: The phytochemical screening of different crude extracts was determined by well-established met...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-09-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2305050014600294 |
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| author | Afaf M. Weli Jamila R.K. AL-Hinai Jawaher M.A. Al-Mjrafi Jawaher R.S. Alnaaimi Mohammad A. Hossain Sadri Saeed Md. S. Aktar |
| author_facet | Afaf M. Weli Jamila R.K. AL-Hinai Jawaher M.A. Al-Mjrafi Jawaher R.S. Alnaaimi Mohammad A. Hossain Sadri Saeed Md. S. Aktar |
| author_sort | Afaf M. Weli |
| collection | DOAJ |
| description | Objective: To prepare different polarities leave crude extracts of Juniperus excels (J. excels) and to determine their phytochemical screening, antioxidant, antimicrobial and cytotoxic activities.
Methods: The phytochemical screening of different crude extracts was determined by well-established methods. The antioxidant activity was determined by free radical scavenging (2,2-diphenyl-1-picrylhydrazyl, DPPH) method. The antimicrobial activity was evaluated by agar disc diffusion method and cytotoxic activity was determined through brine shrimp lethality assay.
Results: The phytochemical tests showed that alkaloids, flavonoids, saponins, steroide, triterpenoids and tannins are present in all leave crude extracts except chloroform crude extract do not contain tannins. The antioxidant results of crude extracts were found to be in the order of hydro alcoholic > chloroform> ethyl acetate >hexane. All leave crude extracts showed moderate antibacterial against gram positive and gram negative food borne pathogen bacteria. The cytotoxicity results revealed that hexane and chloroform extracts have killed all the shrimp larvae at the concentration of 500 μg/mL.
Conclusion: More in-vivo and in-vitro studies along with detailed phytochemical investigations are needed in order to potentially use this plant in the prevention and therapies of some oxidative damage related diseases. |
| first_indexed | 2024-12-10T22:29:59Z |
| format | Article |
| id | doaj.art-524df11d0b944e6988465858ae93f4f7 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 |
| language | English |
| last_indexed | 2024-12-10T22:29:59Z |
| publishDate | 2014-09-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-524df11d0b944e6988465858ae93f4f72022-12-22T01:31:04ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002014-09-013321822310.1016/S2305-0500(14)60029-4Effect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screeningAfaf M. WeliJamila R.K. AL-HinaiJawaher M.A. Al-MjrafiJawaher R.S. AlnaaimiMohammad A. HossainSadri SaeedMd. S. AktarObjective: To prepare different polarities leave crude extracts of Juniperus excels (J. excels) and to determine their phytochemical screening, antioxidant, antimicrobial and cytotoxic activities. Methods: The phytochemical screening of different crude extracts was determined by well-established methods. The antioxidant activity was determined by free radical scavenging (2,2-diphenyl-1-picrylhydrazyl, DPPH) method. The antimicrobial activity was evaluated by agar disc diffusion method and cytotoxic activity was determined through brine shrimp lethality assay. Results: The phytochemical tests showed that alkaloids, flavonoids, saponins, steroide, triterpenoids and tannins are present in all leave crude extracts except chloroform crude extract do not contain tannins. The antioxidant results of crude extracts were found to be in the order of hydro alcoholic > chloroform> ethyl acetate >hexane. All leave crude extracts showed moderate antibacterial against gram positive and gram negative food borne pathogen bacteria. The cytotoxicity results revealed that hexane and chloroform extracts have killed all the shrimp larvae at the concentration of 500 μg/mL. Conclusion: More in-vivo and in-vitro studies along with detailed phytochemical investigations are needed in order to potentially use this plant in the prevention and therapies of some oxidative damage related diseases.http://www.sciencedirect.com/science/article/pii/S2305050014600294Juniperus excelsMacerationPhytochemical screeningAntioxidant activitiesAtimicrobial activitiesCytotoxic activities |
| spellingShingle | Afaf M. Weli Jamila R.K. AL-Hinai Jawaher M.A. Al-Mjrafi Jawaher R.S. Alnaaimi Mohammad A. Hossain Sadri Saeed Md. S. Aktar Effect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening Asian Pacific Journal of Reproduction Juniperus excels Maceration Phytochemical screening Antioxidant activities Atimicrobial activities Cytotoxic activities |
| title | Effect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening |
| title_full | Effect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening |
| title_fullStr | Effect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening |
| title_full_unstemmed | Effect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening |
| title_short | Effect of different polarities leaves crude extracts of Omani juniperus excels on antioxidant, antimicrobial and cytotoxic activities and their biochemical screening |
| title_sort | effect of different polarities leaves crude extracts of omani juniperus excels on antioxidant antimicrobial and cytotoxic activities and their biochemical screening |
| topic | Juniperus excels Maceration Phytochemical screening Antioxidant activities Atimicrobial activities Cytotoxic activities |
| url | http://www.sciencedirect.com/science/article/pii/S2305050014600294 |
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