Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites
IntroductionThe modernized lifestyle has been paralleled by an epidemic of inflammatory bowel disease (IBD). Excessive consumption of cold beverages is especially common among the modern humans. However, whether cold stress contributes directly to the gut barrier and gut–brain axis is not clear.Meth...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1134246/full |
| _version_ | 1827969625107726336 |
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| author | Lijuan Sun Xueying Wang Yuankang Zou Yixuan He Changting Liang Juan Li Pu Li Pu Li Jianbin Zhang |
| author_facet | Lijuan Sun Xueying Wang Yuankang Zou Yixuan He Changting Liang Juan Li Pu Li Pu Li Jianbin Zhang |
| author_sort | Lijuan Sun |
| collection | DOAJ |
| description | IntroductionThe modernized lifestyle has been paralleled by an epidemic of inflammatory bowel disease (IBD). Excessive consumption of cold beverages is especially common among the modern humans. However, whether cold stress contributes directly to the gut barrier and gut–brain axis is not clear.MethodsWe conducted a cold stress model induced by cold water. The mice were treated with 14 consecutive days of intragastric cold or common water administration. We observed changes in gut transit and gut barrier in the colon. We also employed RNA sequencing-based transcriptomic analysis to identify the genes potentially driving gut injury, and simultaneously examined the gut microbiota and metabolites in the feces.ResultsWe found that cold stress disturbed the intestinal function and increased gut permeability. A set of core genes related to immune responses were consistently overexpressed in the cold stress group. Additionally, cold stress induced decreased bacterial diversity, ecological network, and increased pathogens mainly belonging to Proteobacteria. The dopamine signaling pathway-related metabolites were largely reduced in the cold stress group.ConclusionThis study revealed that cold stress could trigger an IBD-like phenotype in mice, implying that cold stress is a possible risk factor for IBD development. |
| first_indexed | 2024-04-09T18:40:33Z |
| format | Article |
| id | doaj.art-52507296624f49ee8e7e27e63086a2bc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T18:40:33Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-52507296624f49ee8e7e27e63086a2bc2023-04-11T05:52:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-04-011410.3389/fmicb.2023.11342461134246Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolitesLijuan Sun0Xueying Wang1Yuankang Zou2Yixuan He3Changting Liang4Juan Li5Pu Li6Pu Li7Jianbin Zhang8Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi’an, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi’an, ChinaDepartment of Occupational and Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi’an, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi’an, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi’an, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi’an, ChinaKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi’an, ChinaDepartment of Anesthesiology, The Second Affiliated Hospital of Air Force Medical University, Xi’an, Shanxi, ChinaDepartment of Occupational and Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment, School of Public Health, Fourth Military Medical University, Xi’an, ChinaIntroductionThe modernized lifestyle has been paralleled by an epidemic of inflammatory bowel disease (IBD). Excessive consumption of cold beverages is especially common among the modern humans. However, whether cold stress contributes directly to the gut barrier and gut–brain axis is not clear.MethodsWe conducted a cold stress model induced by cold water. The mice were treated with 14 consecutive days of intragastric cold or common water administration. We observed changes in gut transit and gut barrier in the colon. We also employed RNA sequencing-based transcriptomic analysis to identify the genes potentially driving gut injury, and simultaneously examined the gut microbiota and metabolites in the feces.ResultsWe found that cold stress disturbed the intestinal function and increased gut permeability. A set of core genes related to immune responses were consistently overexpressed in the cold stress group. Additionally, cold stress induced decreased bacterial diversity, ecological network, and increased pathogens mainly belonging to Proteobacteria. The dopamine signaling pathway-related metabolites were largely reduced in the cold stress group.ConclusionThis study revealed that cold stress could trigger an IBD-like phenotype in mice, implying that cold stress is a possible risk factor for IBD development.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1134246/fullcold stressinflammatory bowel diseasegut microbiotagut–brain axisanimal research |
| spellingShingle | Lijuan Sun Xueying Wang Yuankang Zou Yixuan He Changting Liang Juan Li Pu Li Pu Li Jianbin Zhang Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites Frontiers in Microbiology cold stress inflammatory bowel disease gut microbiota gut–brain axis animal research |
| title | Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites |
| title_full | Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites |
| title_fullStr | Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites |
| title_full_unstemmed | Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites |
| title_short | Cold stress induces colitis-like phenotypes in mice by altering gut microbiota and metabolites |
| title_sort | cold stress induces colitis like phenotypes in mice by altering gut microbiota and metabolites |
| topic | cold stress inflammatory bowel disease gut microbiota gut–brain axis animal research |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1134246/full |
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