Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium
Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating bla...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2020-04-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/31493 |
| _version_ | 1818385123964354560 |
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| author | Vista Budiariati Dwi Budiono Mokhamad Fahrudin Berry Juliandi Ratih Rinendyaputri Arief Boediono |
| author_facet | Vista Budiariati Dwi Budiono Mokhamad Fahrudin Berry Juliandi Ratih Rinendyaputri Arief Boediono |
| author_sort | Vista Budiariati |
| collection | DOAJ |
| description | Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst. |
| first_indexed | 2024-12-14T03:33:10Z |
| format | Article |
| id | doaj.art-5252eba7e4da4cf3b37572dc339e5622 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-12-14T03:33:10Z |
| publishDate | 2020-04-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-5252eba7e4da4cf3b37572dc339e56222022-12-21T23:18:42ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942020-04-0127210.4308/hjb.27.2.89Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free MediumVista Budiariati0Dwi Budiono1Mokhamad Fahrudin2Berry Juliandi3Ratih Rinendyaputri4Arief Boediono5Faculty of Veterinary Medicine, Universitas Gadjah Mada, YogyakartaDepartment of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, BogorDepartment of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, BogorDepartment of Biology, Faculty of Mathematics and Natural Sciences, IPB University, BogorCenter for Research and Development of Biomedical and Basic Health Technology, National Institute of Health Research and Development, Ministry of Health, JakartaDepartment of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University, BogorParthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.https://journal.ipb.ac.id/index.php/hayati/article/view/31493 |
| spellingShingle | Vista Budiariati Dwi Budiono Mokhamad Fahrudin Berry Juliandi Ratih Rinendyaputri Arief Boediono Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium Hayati Journal of Biosciences |
| title | Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium |
| title_full | Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium |
| title_fullStr | Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium |
| title_full_unstemmed | Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium |
| title_short | Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium |
| title_sort | production and characterization of mouse diploid parthenogenetic blastocyst developed in phosphate free medium |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/31493 |
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