Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report
Objective: Prostate carcinoma is a serious public health in men with increasing incidence and mortality rates. The present study aimed to investigate cytotoxic activities of silver nanoparticles (AgNPs) against PC-3 human prostate cancer cell line in vitro. Materials and Methods: The antiproliferat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Verduci Editore
2022-03-01
|
| Series: | World Cancer Research Journal |
| Subjects: | |
| Online Access: | https://www.wcrj.net/wp-content/uploads/sites/5/2022/03/e2242.pdf |
| _version_ | 1811293453506576384 |
|---|---|
| author | A. Jafari Z. Niknam M. Rahimi H. Zali M. Rezaei-Tavirani |
| author_facet | A. Jafari Z. Niknam M. Rahimi H. Zali M. Rezaei-Tavirani |
| author_sort | A. Jafari |
| collection | DOAJ |
| description | Objective: Prostate carcinoma is a serious public health in men with increasing incidence and mortality rates. The present study aimed to investigate cytotoxic activities of silver nanoparticles (AgNPs) against PC-3 human prostate cancer cell line in vitro.
Materials and Methods: The antiproliferative effect of AgNPs on PC-3 cells was assessed using various concentrations of AgNPs (5-80 µg/mL) by MTT assay. We have also investigated the effect of AgNPs on the migration property of cancers cells using a wound-healing assay. Then, obtained results were analyzed using ANOVA and Student’s t-test.
Results: The AgNPs diminished significantly the viability of PC-3 cells in a dose and time-dependent manner. According to the dose-dependent viability curve, the IC50 (50% inhibiting concentration) at 48 h was calculated to be 65.7 µg/mL. Migration assay also confirmed the antimetastatic potential of the AgNPs against PC-3 cells. After 24 h, the migration rate was 2.5 fold lower for AgNPs-treated cells compared to control cells.
Conclusions: Our findings support the use of AgNPs as an appropriate option for future therapeutic application in prostate cancer therapy. |
| first_indexed | 2024-04-13T05:01:38Z |
| format | Article |
| id | doaj.art-525392b2c8da4f7b811c508d13101a2a |
| institution | Directory Open Access Journal |
| issn | 2372-3416 |
| language | English |
| last_indexed | 2024-04-13T05:01:38Z |
| publishDate | 2022-03-01 |
| publisher | Verduci Editore |
| record_format | Article |
| series | World Cancer Research Journal |
| spelling | doaj.art-525392b2c8da4f7b811c508d13101a2a2022-12-22T03:01:19ZengVerduci EditoreWorld Cancer Research Journal2372-34162022-03-01910.32113/wcrj_20223_22422242Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental reportA. Jafari0Z. Niknam1M. Rahimi2H. Zali3M. Rezaei-Tavirani4Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, IranProteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IranResearch Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranProteomics Research Center, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, IranObjective: Prostate carcinoma is a serious public health in men with increasing incidence and mortality rates. The present study aimed to investigate cytotoxic activities of silver nanoparticles (AgNPs) against PC-3 human prostate cancer cell line in vitro. Materials and Methods: The antiproliferative effect of AgNPs on PC-3 cells was assessed using various concentrations of AgNPs (5-80 µg/mL) by MTT assay. We have also investigated the effect of AgNPs on the migration property of cancers cells using a wound-healing assay. Then, obtained results were analyzed using ANOVA and Student’s t-test. Results: The AgNPs diminished significantly the viability of PC-3 cells in a dose and time-dependent manner. According to the dose-dependent viability curve, the IC50 (50% inhibiting concentration) at 48 h was calculated to be 65.7 µg/mL. Migration assay also confirmed the antimetastatic potential of the AgNPs against PC-3 cells. After 24 h, the migration rate was 2.5 fold lower for AgNPs-treated cells compared to control cells. Conclusions: Our findings support the use of AgNPs as an appropriate option for future therapeutic application in prostate cancer therapy.https://www.wcrj.net/wp-content/uploads/sites/5/2022/03/e2242.pdfprostate cancersilver nanoparticlesviabilitymetastasis |
| spellingShingle | A. Jafari Z. Niknam M. Rahimi H. Zali M. Rezaei-Tavirani Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report World Cancer Research Journal prostate cancer silver nanoparticles viability metastasis |
| title | Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report |
| title_full | Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report |
| title_fullStr | Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report |
| title_full_unstemmed | Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report |
| title_short | Silver nanoparticles inhibit proliferation and migration of prostate cancer cells: an experimental report |
| title_sort | silver nanoparticles inhibit proliferation and migration of prostate cancer cells an experimental report |
| topic | prostate cancer silver nanoparticles viability metastasis |
| url | https://www.wcrj.net/wp-content/uploads/sites/5/2022/03/e2242.pdf |
| work_keys_str_mv | AT ajafari silvernanoparticlesinhibitproliferationandmigrationofprostatecancercellsanexperimentalreport AT zniknam silvernanoparticlesinhibitproliferationandmigrationofprostatecancercellsanexperimentalreport AT mrahimi silvernanoparticlesinhibitproliferationandmigrationofprostatecancercellsanexperimentalreport AT hzali silvernanoparticlesinhibitproliferationandmigrationofprostatecancercellsanexperimentalreport AT mrezaeitavirani silvernanoparticlesinhibitproliferationandmigrationofprostatecancercellsanexperimentalreport |