Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response
In addition to their role in the biosynthesis of important molecules such as proteins and specialized metabolites, amino acids are known to function as signaling molecules through various pathways to report nitrogen status and trigger appropriate metabolic and cellular responses. Moreover, changes i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2020.606386/full |
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| author | Julien Besnard Julien Besnard Unnati Sonawala Bal Maharjan Eva Collakova Scott A. Finlayson Scott A. Finlayson Guillaume Pilot John McDowell Sakiko Okumoto Sakiko Okumoto |
| author_facet | Julien Besnard Julien Besnard Unnati Sonawala Bal Maharjan Eva Collakova Scott A. Finlayson Scott A. Finlayson Guillaume Pilot John McDowell Sakiko Okumoto Sakiko Okumoto |
| author_sort | Julien Besnard |
| collection | DOAJ |
| description | In addition to their role in the biosynthesis of important molecules such as proteins and specialized metabolites, amino acids are known to function as signaling molecules through various pathways to report nitrogen status and trigger appropriate metabolic and cellular responses. Moreover, changes in amino acid levels through altered amino acid transporter activities trigger plant immune responses. Specifically, loss of function of major amino acid transporter, over-expression of cationic amino acid transporter, or over-expression of the positive regulators of membrane amino acid export all lead to dwarfed phenotypes and upregulated salicylic acid (SA)-induced stress marker genes. However, whether increasing amino acid exporter protein levels lead to similar stress phenotypes has not been investigated so far. Recently, a family of transporters, namely USUALLY MULTIPLE ACIDS MOVE IN AND OUT TRANSPORTERS (UMAMITs), were identified as amino acid exporters. The goal of this study was to investigate the effects of increased amino acid export on plant development, growth, and reproduction to further examine the link between amino acid transport and stress responses. The results presented here show strong evidence that an increased expression of UMAMIT transporters induces stress phenotypes and pathogen resistance, likely due to the establishment of a constitutive stress response via a SA-dependent pathway. |
| first_indexed | 2024-12-14T22:26:07Z |
| format | Article |
| id | doaj.art-52565a4351cd46478a312f93bde77a05 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-14T22:26:07Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-52565a4351cd46478a312f93bde77a052022-12-21T22:45:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-01-011110.3389/fpls.2020.606386606386Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress ResponseJulien Besnard0Julien Besnard1Unnati Sonawala2Bal Maharjan3Eva Collakova4Scott A. Finlayson5Scott A. Finlayson6Guillaume Pilot7John McDowell8Sakiko Okumoto9Sakiko Okumoto10Department of Plant Pathology, Physiology and Weed Science, Virginia Tech, Blacksburg, VA, United StatesDepartment of Soil and Crop Sciences, Texas A&M AgriLife Research, Texas A&M University, College Station, TX, United StatesDepartment of Plant Pathology, Physiology and Weed Science, Virginia Tech, Blacksburg, VA, United StatesDepartment of Soil and Crop Sciences, Texas A&M AgriLife Research, Texas A&M University, College Station, TX, United StatesSchool of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, United StatesDepartment of Soil and Crop Sciences, Texas A&M AgriLife Research, Texas A&M University, College Station, TX, United StatesFaculty of Molecular and Environmental Plant Sciences, Texas A&M University, College Station, TX, United StatesSchool of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, United StatesSchool of Plant and Environmental Sciences, Virginia Tech, Blacksburg, VA, United StatesDepartment of Plant Pathology, Physiology and Weed Science, Virginia Tech, Blacksburg, VA, United StatesDepartment of Soil and Crop Sciences, Texas A&M AgriLife Research, Texas A&M University, College Station, TX, United StatesIn addition to their role in the biosynthesis of important molecules such as proteins and specialized metabolites, amino acids are known to function as signaling molecules through various pathways to report nitrogen status and trigger appropriate metabolic and cellular responses. Moreover, changes in amino acid levels through altered amino acid transporter activities trigger plant immune responses. Specifically, loss of function of major amino acid transporter, over-expression of cationic amino acid transporter, or over-expression of the positive regulators of membrane amino acid export all lead to dwarfed phenotypes and upregulated salicylic acid (SA)-induced stress marker genes. However, whether increasing amino acid exporter protein levels lead to similar stress phenotypes has not been investigated so far. Recently, a family of transporters, namely USUALLY MULTIPLE ACIDS MOVE IN AND OUT TRANSPORTERS (UMAMITs), were identified as amino acid exporters. The goal of this study was to investigate the effects of increased amino acid export on plant development, growth, and reproduction to further examine the link between amino acid transport and stress responses. The results presented here show strong evidence that an increased expression of UMAMIT transporters induces stress phenotypes and pathogen resistance, likely due to the establishment of a constitutive stress response via a SA-dependent pathway.https://www.frontiersin.org/articles/10.3389/fpls.2020.606386/fullmembrane transportamino acid transportamino acid metabolismstress responsesalicylic acid |
| spellingShingle | Julien Besnard Julien Besnard Unnati Sonawala Bal Maharjan Eva Collakova Scott A. Finlayson Scott A. Finlayson Guillaume Pilot John McDowell Sakiko Okumoto Sakiko Okumoto Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response Frontiers in Plant Science membrane transport amino acid transport amino acid metabolism stress response salicylic acid |
| title | Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response |
| title_full | Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response |
| title_fullStr | Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response |
| title_full_unstemmed | Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response |
| title_short | Increased Expression of UMAMIT Amino Acid Transporters Results in Activation of Salicylic Acid Dependent Stress Response |
| title_sort | increased expression of umamit amino acid transporters results in activation of salicylic acid dependent stress response |
| topic | membrane transport amino acid transport amino acid metabolism stress response salicylic acid |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2020.606386/full |
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