Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector?
Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by <i>Pasteurella</i> (<i>P</i>.) <i>multocida</i> capsular type B (<i>Pm</i>B) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf...
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MDPI AG
2021-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1999 |
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| author | Peter Kutzer Claudia A. Szentiks Sabine Bock Guido Fritsch Tibor Magyar Christoph Schulze Torsten Semmler Christa Ewers |
| author_facet | Peter Kutzer Claudia A. Szentiks Sabine Bock Guido Fritsch Tibor Magyar Christoph Schulze Torsten Semmler Christa Ewers |
| author_sort | Peter Kutzer |
| collection | DOAJ |
| description | Since 2010, outbreaks of haemorrhagic septicaemia (HS) caused by <i>Pasteurella</i> (<i>P</i>.) <i>multocida</i> capsular type B (<i>Pm</i>B) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf (<i>Canis lupus</i>) territories. Thus, the main objectives of our study were to investigate the molecular epidemiology of German <i>Pm</i>B-HS-isolates and to assess the role of wolves as putative vectors of this pathogen. We collected 83 <i>Pm</i>B isolates from HS outbreaks that occurred between 2010 and 2019 and sampled 150 wolves, which were found dead in the years 2017 to 2019, revealing another three <i>Pm</i>B isolates. A maximum-likelihood-based phylogeny of the core genomes of 65 <i>Pm</i>B-HS-isolates and the three <i>Pm</i>B-wolf-isolates showed high relatedness. Furthermore, all belonged to capsular:LPS:MLST genotype B:L2:ST122<sup>RIRDC</sup> and showed highly similar virulence gene profiles, but clustered separately from 35 global ST122<sup>RIRDC</sup> strains. Our data revealed that German HS outbreaks were caused by a distinct genomic lineage of <i>Pm</i>B-ST122 strains, hinting towards an independent, ongoing epidemiologic event. We demonstrated for the first time, that carnivores, i.e., wolves, might harbour <i>Pm</i>B as a part of their oropharyngeal microbiota. Furthermore, the results of our study imply that wolves can carry the pathogen over long distances, indicating a major role of that animal species in the ongoing epidemiological event of HS in Germany. |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:02Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-525a34161f85449cb67615a769aba76d2023-11-22T14:20:21ZengMDPI AGMicroorganisms2076-26072021-09-0199199910.3390/microorganisms9091999Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector?Peter Kutzer0Claudia A. Szentiks1Sabine Bock2Guido Fritsch3Tibor Magyar4Christoph Schulze5Torsten Semmler6Christa Ewers7Landeslabor Berlin-Brandenburg, 15236 Frankfurt (Oder), GermanyDepartment of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, 10315 Berlin, GermanyLandeslabor Berlin-Brandenburg, 15236 Frankfurt (Oder), GermanyDepartment of Reproduction Management, Leibniz Institute for Zoo and Wildlife Research, 10315 Berlin, GermanyVeterinary Medical Research Institute, Eötvös Lóránd Research Network (ELKH), 1143 Budapest, HungaryLandeslabor Berlin-Brandenburg, 15236 Frankfurt (Oder), GermanyMicrobial Genomics, Robert Koch Institute, 13353 Berlin, GermanyFaculty of Veterinary Medicine, Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, 35392 Giessen, GermanySince 2010, outbreaks of haemorrhagic septicaemia (HS) caused by <i>Pasteurella</i> (<i>P</i>.) <i>multocida</i> capsular type B (<i>Pm</i>B) emerged in Germany. In 2017, we noticed a close spatiotemporal relationship between HS outbreak sites and wolf (<i>Canis lupus</i>) territories. Thus, the main objectives of our study were to investigate the molecular epidemiology of German <i>Pm</i>B-HS-isolates and to assess the role of wolves as putative vectors of this pathogen. We collected 83 <i>Pm</i>B isolates from HS outbreaks that occurred between 2010 and 2019 and sampled 150 wolves, which were found dead in the years 2017 to 2019, revealing another three <i>Pm</i>B isolates. A maximum-likelihood-based phylogeny of the core genomes of 65 <i>Pm</i>B-HS-isolates and the three <i>Pm</i>B-wolf-isolates showed high relatedness. Furthermore, all belonged to capsular:LPS:MLST genotype B:L2:ST122<sup>RIRDC</sup> and showed highly similar virulence gene profiles, but clustered separately from 35 global ST122<sup>RIRDC</sup> strains. Our data revealed that German HS outbreaks were caused by a distinct genomic lineage of <i>Pm</i>B-ST122 strains, hinting towards an independent, ongoing epidemiologic event. We demonstrated for the first time, that carnivores, i.e., wolves, might harbour <i>Pm</i>B as a part of their oropharyngeal microbiota. Furthermore, the results of our study imply that wolves can carry the pathogen over long distances, indicating a major role of that animal species in the ongoing epidemiological event of HS in Germany.https://www.mdpi.com/2076-2607/9/9/1999<i>Pasteurella multocida</i>wild boardomestic animalscore genomeMLSTvirulence |
| spellingShingle | Peter Kutzer Claudia A. Szentiks Sabine Bock Guido Fritsch Tibor Magyar Christoph Schulze Torsten Semmler Christa Ewers Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? Microorganisms <i>Pasteurella multocida</i> wild boar domestic animals core genome MLST virulence |
| title | Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
| title_full | Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
| title_fullStr | Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
| title_full_unstemmed | Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
| title_short | Re-Emergence and Spread of Haemorrhagic Septicaemia in Germany: The Wolf as a Vector? |
| title_sort | re emergence and spread of haemorrhagic septicaemia in germany the wolf as a vector |
| topic | <i>Pasteurella multocida</i> wild boar domestic animals core genome MLST virulence |
| url | https://www.mdpi.com/2076-2607/9/9/1999 |
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