The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum
The main problem in processing bovine colostrum is preserving as many beneficial compounds as possible, most of which have low thermal stability. The present study evaluates the possibility of using cold plasma (CP) as a decontamination technology and its effect on selected biologically active fract...
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MDPI AG
2023-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/9/5490 |
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| author | Elżbieta Bogusławska-Wąs Alicja Dłubała Wojciech Sawicki Małgorzata Ożgo Adam Lepczyński |
| author_facet | Elżbieta Bogusławska-Wąs Alicja Dłubała Wojciech Sawicki Małgorzata Ożgo Adam Lepczyński |
| author_sort | Elżbieta Bogusławska-Wąs |
| collection | DOAJ |
| description | The main problem in processing bovine colostrum is preserving as many beneficial compounds as possible, most of which have low thermal stability. The present study evaluates the possibility of using cold plasma (CP) as a decontamination technology and its effect on selected biologically active fractions of freeze-dried bovine colostrum. The plasma process was carried out in air, nitrogen, and oxygen environments. The results revealed that the sterilization process using CP caused slight changes in the colour of the samples expressed by the attributes ΔC, ΔL, Δh and ΔE. The decontamination effect depended on the gas used and the type of microorganism. The highest decontamination effects were gained under oxygen conditions, where reductions were obtained for total psychrophilic bacteria (THPC) by log 1.24, mesophilic bacteria (THMC) by log 1.02, <i>Enterobacteriaceae</i> by log 1.16, <i>E. coli</i> by log 0.96, yeast (TYMC) by log 0.92. A significantly lower decontaminating effect was obtained for Gram-positive bacteria and sporophytic forms. Additionally, the application of CP, regardless of the gas used, affected the modification of protein structure and reduction of immunoglobulin concentration. as proven by proteomics analyses (1-DE, 2-DE, MALDI–TOF MS). The same applied to β-lactoglobulin in air and oxygen and BSA in nitrogen and air. |
| first_indexed | 2024-03-11T04:24:07Z |
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| id | doaj.art-525c84cfdce14e6992d281fde4357ee7 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T04:24:07Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-525c84cfdce14e6992d281fde4357ee72023-11-17T22:34:44ZengMDPI AGApplied Sciences2076-34172023-04-01139549010.3390/app13095490The Effect of Cold Plasma on Selected Parameters of Bovine ColostrumElżbieta Bogusławska-Wąs0Alicja Dłubała1Wojciech Sawicki2Małgorzata Ożgo3Adam Lepczyński4Department of Applied Microbiology and Human Nutrition Physiology, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology Szczecin, Papieża Pawła VI 3, 71-459 Szczecin, PolandDepartment of Applied Microbiology and Human Nutrition Physiology, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology Szczecin, Papieża Pawła VI 3, 71-459 Szczecin, PolandDepartment of Applied Microbiology and Human Nutrition Physiology, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology Szczecin, Papieża Pawła VI 3, 71-459 Szczecin, PolandDepartment of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, PolandDepartment of Physiology, Cytobiology and Proteomics, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology Szczecin, Klemensa Janickiego 29, 71-270 Szczecin, PolandThe main problem in processing bovine colostrum is preserving as many beneficial compounds as possible, most of which have low thermal stability. The present study evaluates the possibility of using cold plasma (CP) as a decontamination technology and its effect on selected biologically active fractions of freeze-dried bovine colostrum. The plasma process was carried out in air, nitrogen, and oxygen environments. The results revealed that the sterilization process using CP caused slight changes in the colour of the samples expressed by the attributes ΔC, ΔL, Δh and ΔE. The decontamination effect depended on the gas used and the type of microorganism. The highest decontamination effects were gained under oxygen conditions, where reductions were obtained for total psychrophilic bacteria (THPC) by log 1.24, mesophilic bacteria (THMC) by log 1.02, <i>Enterobacteriaceae</i> by log 1.16, <i>E. coli</i> by log 0.96, yeast (TYMC) by log 0.92. A significantly lower decontaminating effect was obtained for Gram-positive bacteria and sporophytic forms. Additionally, the application of CP, regardless of the gas used, affected the modification of protein structure and reduction of immunoglobulin concentration. as proven by proteomics analyses (1-DE, 2-DE, MALDI–TOF MS). The same applied to β-lactoglobulin in air and oxygen and BSA in nitrogen and air.https://www.mdpi.com/2076-3417/13/9/5490cold plasmacolostrumproteomedecontamination technologymicrobiological safety |
| spellingShingle | Elżbieta Bogusławska-Wąs Alicja Dłubała Wojciech Sawicki Małgorzata Ożgo Adam Lepczyński The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum Applied Sciences cold plasma colostrum proteome decontamination technology microbiological safety |
| title | The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum |
| title_full | The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum |
| title_fullStr | The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum |
| title_full_unstemmed | The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum |
| title_short | The Effect of Cold Plasma on Selected Parameters of Bovine Colostrum |
| title_sort | effect of cold plasma on selected parameters of bovine colostrum |
| topic | cold plasma colostrum proteome decontamination technology microbiological safety |
| url | https://www.mdpi.com/2076-3417/13/9/5490 |
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