Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort study
Abstract Background Microparticles (MPs) are small (<1 μm) cell membrane-derived vesicles that are formed in response to cellular activation or early stages of apoptosis. Increased plasma MP levels have been associated with liver disease severity. Here we investigated the clinical impact of ascit...
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-017-1288-3 |
| _version_ | 1819022717229203456 |
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| author | Cornelius Engelmann Katrin Splith Sandra Krohn Adam Herber Albrecht Boehlig Stephan Boehm Johann Pratschke Thomas Berg Moritz Schmelzle |
| author_facet | Cornelius Engelmann Katrin Splith Sandra Krohn Adam Herber Albrecht Boehlig Stephan Boehm Johann Pratschke Thomas Berg Moritz Schmelzle |
| author_sort | Cornelius Engelmann |
| collection | DOAJ |
| description | Abstract Background Microparticles (MPs) are small (<1 μm) cell membrane-derived vesicles that are formed in response to cellular activation or early stages of apoptosis. Increased plasma MP levels have been associated with liver disease severity. Here we investigated the clinical impact of ascites MPs in patients with decompensated liver cirrhosis. Methods Ascites and blood samples of 163 patients with cirrhosis (ascites n = 163, blood n = 31) were collected between February 2011 and December 2012. MPs were obtained from ascites and from blood by two-step ultracentrifugation and quantified by flow cytometry. Quantitative absolute MP levels were correlated with clinical and laboratory baseline parameters as well as patient outcomes. Ascites microparticles were stained with antibodies against CD66b (neutrophils) and CD3 (lymphocytes) in a subgroup of 60 matched patients. Results MPs were detected in all ascites and blood samples. Absolute ascites MP levels correlated with blood levels (r = 0.444, p = 0.011). Low ascites MP levels (<488.4 MP/μL) were associated with a poor 30-day survival probability (<488.4 MP/μL 71.1% vs. >488.4 MP/μL 94.7%, log rank p = 0.001) and such patients had a higher relative amount of ascites microparticles derived from neutrophils and lymphocytes. Low levels of ascites MPs, high MELD score and antibiotic treatment were independent risk factors for death within 30 days. Conclusions Ascites MP levels predict short-term survival along with the liver function in patients with decompensated cirrhosis. Further studies which evaluate ascites MPs as disease specific biomarker with a validation cohort and which investigate its underlying mechanisms are needed. Neutrophils and lymphocytes contributed more frequently to the release of microparticles in patients with low ascites levels, possibly indicating an immune activation in this cohort. |
| first_indexed | 2024-12-21T04:27:26Z |
| format | Article |
| id | doaj.art-525c94c5cd3c4dfab587f27e6741e903 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-21T04:27:26Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-525c94c5cd3c4dfab587f27e6741e9032022-12-21T19:16:01ZengBMCJournal of Translational Medicine1479-58762017-09-0115111010.1186/s12967-017-1288-3Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort studyCornelius Engelmann0Katrin Splith1Sandra Krohn2Adam Herber3Albrecht Boehlig4Stephan Boehm5Johann Pratschke6Thomas Berg7Moritz Schmelzle8Section of Hepatology, Department of Internal Medicine, Neurology, Dermatology, University Hospital LeipzigDepartment of Surgery, Campus Virchow Klinikum, Charité-Universitätsmedizin BerlinSection of Hepatology, Department of Internal Medicine, Neurology, Dermatology, University Hospital LeipzigSection of Hepatology, Department of Internal Medicine, Neurology, Dermatology, University Hospital LeipzigSection of Hepatology, Department of Internal Medicine, Neurology, Dermatology, University Hospital LeipzigKlinische Virologie, Max von Pettenkofer-Institut, Medizinische Fakultät, Ludwig-Maximilians-Universität MünchenDepartment of Surgery, Campus Virchow Klinikum, Charité-Universitätsmedizin BerlinSection of Hepatology, Department of Internal Medicine, Neurology, Dermatology, University Hospital LeipzigDepartment of Surgery, Campus Virchow Klinikum, Charité-Universitätsmedizin BerlinAbstract Background Microparticles (MPs) are small (<1 μm) cell membrane-derived vesicles that are formed in response to cellular activation or early stages of apoptosis. Increased plasma MP levels have been associated with liver disease severity. Here we investigated the clinical impact of ascites MPs in patients with decompensated liver cirrhosis. Methods Ascites and blood samples of 163 patients with cirrhosis (ascites n = 163, blood n = 31) were collected between February 2011 and December 2012. MPs were obtained from ascites and from blood by two-step ultracentrifugation and quantified by flow cytometry. Quantitative absolute MP levels were correlated with clinical and laboratory baseline parameters as well as patient outcomes. Ascites microparticles were stained with antibodies against CD66b (neutrophils) and CD3 (lymphocytes) in a subgroup of 60 matched patients. Results MPs were detected in all ascites and blood samples. Absolute ascites MP levels correlated with blood levels (r = 0.444, p = 0.011). Low ascites MP levels (<488.4 MP/μL) were associated with a poor 30-day survival probability (<488.4 MP/μL 71.1% vs. >488.4 MP/μL 94.7%, log rank p = 0.001) and such patients had a higher relative amount of ascites microparticles derived from neutrophils and lymphocytes. Low levels of ascites MPs, high MELD score and antibiotic treatment were independent risk factors for death within 30 days. Conclusions Ascites MP levels predict short-term survival along with the liver function in patients with decompensated cirrhosis. Further studies which evaluate ascites MPs as disease specific biomarker with a validation cohort and which investigate its underlying mechanisms are needed. Neutrophils and lymphocytes contributed more frequently to the release of microparticles in patients with low ascites levels, possibly indicating an immune activation in this cohort.http://link.springer.com/article/10.1186/s12967-017-1288-3CirrhosisAscitesMicroparticlesMP |
| spellingShingle | Cornelius Engelmann Katrin Splith Sandra Krohn Adam Herber Albrecht Boehlig Stephan Boehm Johann Pratschke Thomas Berg Moritz Schmelzle Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort study Journal of Translational Medicine Cirrhosis Ascites Microparticles MP |
| title | Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort study |
| title_full | Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort study |
| title_fullStr | Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort study |
| title_full_unstemmed | Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort study |
| title_short | Absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis: a cohort study |
| title_sort | absolute quantification of microparticles by flow cytometry in ascites of patients with decompensated cirrhosis a cohort study |
| topic | Cirrhosis Ascites Microparticles MP |
| url | http://link.springer.com/article/10.1186/s12967-017-1288-3 |
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