Small RNAs Virome Characterization Reveals Arthropod-Associated Viruses in <i>Anopheles atroparvus </i>from the Ebro Delta, Spain
Even though malaria was eradicated from Europe after the mid-20th century, in 2017, more than 8000 imported cases were reported in the continent. Due to travel routes to endemic areas, climate change, and the presence of native vector mosquitoes (genus <i>Anopheles</i>), the re-establish...
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MDPI AG
2020-06-01
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| author | Lotty Birnberg Francisco M. Codoñer Raúl Escosa Carles Aranda Ana Isabel Núñez Sandra Talavera Núria Busquets |
| author_facet | Lotty Birnberg Francisco M. Codoñer Raúl Escosa Carles Aranda Ana Isabel Núñez Sandra Talavera Núria Busquets |
| author_sort | Lotty Birnberg |
| collection | DOAJ |
| description | Even though malaria was eradicated from Europe after the mid-20th century, in 2017, more than 8000 imported cases were reported in the continent. Due to travel routes to endemic areas, climate change, and the presence of native vector mosquitoes (genus <i>Anopheles</i>), the re-establishment of autochthonous malaria transmission is a current concern. <i>Anopheles atroparvus</i> (Van Thiel, 1972) is one of the 11 sibling species within the Palearctic <i>Anopheles maculipennis</i> complex, which formerly were considered the main vectors of the disease in the European continent. The microbiota (bacteria and viruses) of vector species has been demonstrated to play a significant role in the biology of these organisms, including their infection susceptibility and their capacity to transmit disease-causing agents. Recently, with the improvement of metagenomics techniques, several viruses that naturally infect vector mosquitoes have been identified. The purpose of the present study was to characterize, for the first time, the virome present in <i>An. atroparvus </i>from the Ebro Delta and assess its evolution after ten generations in the laboratory. Small RNA sequencing was used to characterize the virome from wild-caught <i>An. atroparvus</i> females and from the tenth generation produced under controlled laboratory conditions. Through this approach, we were able to identify viral linages previously reported in other invertebrates, such as Chaq virus and several Partiti-like viruses. A reduction in the viral composition was observed during the colonization process. The present study contributes to the understanding of the viral diversity of a medically relevant vector species in its natural setting and under confinement, and sets a baseline for further studies to assess the potential implications of these viruses in the transmission of pathogens. |
| first_indexed | 2024-03-10T18:56:13Z |
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| language | English |
| last_indexed | 2024-03-10T18:56:13Z |
| publishDate | 2020-06-01 |
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| series | Proceedings |
| spelling | doaj.art-525d906236db43eaba84730713597adf2023-11-20T04:46:33ZengMDPI AGProceedings2504-39002020-06-0150110310.3390/proceedings2020050103Small RNAs Virome Characterization Reveals Arthropod-Associated Viruses in <i>Anopheles atroparvus </i>from the Ebro Delta, SpainLotty Birnberg0Francisco M. Codoñer1Raúl Escosa2Carles Aranda3Ana Isabel Núñez4Sandra Talavera5Núria Busquets6IRTA, Centre de Recerca en Sanitat Animal (IRTA-CReSA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainLifesequencing, Parc Cientific Universitat de Valencia, 46980 Paterna, SpainConsorci de Politiques Ambientals de les Terres de l’Ebre (COPATE), Plaça de Lluis Companys, 43870 Amposta, SpainIRTA, Centre de Recerca en Sanitat Animal (IRTA-CReSA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainIRTA, Centre de Recerca en Sanitat Animal (IRTA-CReSA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainIRTA, Centre de Recerca en Sanitat Animal (IRTA-CReSA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainIRTA, Centre de Recerca en Sanitat Animal (IRTA-CReSA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainEven though malaria was eradicated from Europe after the mid-20th century, in 2017, more than 8000 imported cases were reported in the continent. Due to travel routes to endemic areas, climate change, and the presence of native vector mosquitoes (genus <i>Anopheles</i>), the re-establishment of autochthonous malaria transmission is a current concern. <i>Anopheles atroparvus</i> (Van Thiel, 1972) is one of the 11 sibling species within the Palearctic <i>Anopheles maculipennis</i> complex, which formerly were considered the main vectors of the disease in the European continent. The microbiota (bacteria and viruses) of vector species has been demonstrated to play a significant role in the biology of these organisms, including their infection susceptibility and their capacity to transmit disease-causing agents. Recently, with the improvement of metagenomics techniques, several viruses that naturally infect vector mosquitoes have been identified. The purpose of the present study was to characterize, for the first time, the virome present in <i>An. atroparvus </i>from the Ebro Delta and assess its evolution after ten generations in the laboratory. Small RNA sequencing was used to characterize the virome from wild-caught <i>An. atroparvus</i> females and from the tenth generation produced under controlled laboratory conditions. Through this approach, we were able to identify viral linages previously reported in other invertebrates, such as Chaq virus and several Partiti-like viruses. A reduction in the viral composition was observed during the colonization process. The present study contributes to the understanding of the viral diversity of a medically relevant vector species in its natural setting and under confinement, and sets a baseline for further studies to assess the potential implications of these viruses in the transmission of pathogens.https://www.mdpi.com/2504-3900/50/1/103Anopheles atroparvussmall RNAChaq virusPartiti-like virusesarthropodviruses |
| spellingShingle | Lotty Birnberg Francisco M. Codoñer Raúl Escosa Carles Aranda Ana Isabel Núñez Sandra Talavera Núria Busquets Small RNAs Virome Characterization Reveals Arthropod-Associated Viruses in <i>Anopheles atroparvus </i>from the Ebro Delta, Spain Proceedings Anopheles atroparvus small RNA Chaq virus Partiti-like viruses arthropod viruses |
| title | Small RNAs Virome Characterization Reveals Arthropod-Associated Viruses in <i>Anopheles atroparvus </i>from the Ebro Delta, Spain |
| title_full | Small RNAs Virome Characterization Reveals Arthropod-Associated Viruses in <i>Anopheles atroparvus </i>from the Ebro Delta, Spain |
| title_fullStr | Small RNAs Virome Characterization Reveals Arthropod-Associated Viruses in <i>Anopheles atroparvus </i>from the Ebro Delta, Spain |
| title_full_unstemmed | Small RNAs Virome Characterization Reveals Arthropod-Associated Viruses in <i>Anopheles atroparvus </i>from the Ebro Delta, Spain |
| title_short | Small RNAs Virome Characterization Reveals Arthropod-Associated Viruses in <i>Anopheles atroparvus </i>from the Ebro Delta, Spain |
| title_sort | small rnas virome characterization reveals arthropod associated viruses in i anopheles atroparvus i from the ebro delta spain |
| topic | Anopheles atroparvus small RNA Chaq virus Partiti-like viruses arthropod viruses |
| url | https://www.mdpi.com/2504-3900/50/1/103 |
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