Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection
miRNAs, small non-coding RNAs that regulate gene expression, are involved in various pathological processes, including viral infections. Virus infections may interfere with the miRNA pathway through the inhibition of genes involved in miRNA biogenesis. A reduction in the number and the levels of miR...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/5/1177 |
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| author | Nathalie Garnier Famara Sane Layal Massara Fabrice Soncin Philippe Gosset Didier Hober Sabine Szunerits Ilka Engelmann |
| author_facet | Nathalie Garnier Famara Sane Layal Massara Fabrice Soncin Philippe Gosset Didier Hober Sabine Szunerits Ilka Engelmann |
| author_sort | Nathalie Garnier |
| collection | DOAJ |
| description | miRNAs, small non-coding RNAs that regulate gene expression, are involved in various pathological processes, including viral infections. Virus infections may interfere with the miRNA pathway through the inhibition of genes involved in miRNA biogenesis. A reduction in the number and the levels of miRNAs expressed in nasopharyngeal swabs of patients with severe COVID-19 was lately observed by us, pointing towards the potential of miRNAs as possible diagnostic or prognostic biomarkers for predicting outcomes among patients with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The objective of the present study was to investigate whether SARS-CoV-2 infection influences the expression levels of messenger RNAs (mRNAs) of key genes involved in miRNA biogenesis. mRNA levels of <i>AGO2</i>, <i>DICER1</i>, <i>DGCR8</i>, <i>DROSHA</i>, and Exportin-5 (<i>XPO5</i>) were measured by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) in nasopharyngeal swab specimens from patients with COVID-19 and controls, as well as in cells infected with SARS-CoV-2 in vitro. Our data showed that the mRNA expression levels of <i>AGO2</i>, <i>DICER1</i>, <i>DGCR8</i>, <i>DROSHA</i>, and <i>XPO5</i> were not significantly different in patients with severe COVID-19 when compared to patients with non-severe COVID-19 and controls. Similarly, the mRNA expression of these genes was not affected by SARS-CoV-2 infection in NHBE and Calu-3 cells. However, in Vero E6 cells, <i>AGO2</i>, <i>DICER1</i>, <i>DGCR8</i>, and <i>XPO5</i> mRNA levels were slightly upregulated 24 h after infection with SARS-CoV-2. In conclusion, we did not find evidence for downregulation of mRNA levels of miRNA biogenesis genes during SARS-CoV-2 infection, neither ex vivo nor in vitro. |
| first_indexed | 2024-03-11T03:14:20Z |
| format | Article |
| id | doaj.art-525dbea4b02840b393bb3dcf62b4b470 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T03:14:20Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-525dbea4b02840b393bb3dcf62b4b4702023-11-18T03:40:06ZengMDPI AGViruses1999-49152023-05-01155117710.3390/v15051177Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 InfectionNathalie Garnier0Famara Sane1Layal Massara2Fabrice Soncin3Philippe Gosset4Didier Hober5Sabine Szunerits6Ilka Engelmann7Laboratoire de Virologie ULR3610, University Lille and CHU Lille, F-59000 Lille, FranceLaboratoire de Virologie ULR3610, University Lille and CHU Lille, F-59000 Lille, FranceCNRS UMR 9017, Inserm U1019, CHU Lille, Institut Pasteur de Lille, CIIL—OpInfIELD, University Lille, F-59000 Lille, FranceCNRS/IIS/Centre Oscar Lambret/Lille University SMMiL-E Project, CNRS Délégation Hauts-de-France, F-59000 Lille, FranceCNRS UMR 9017, Inserm U1019, CHU Lille, Institut Pasteur de Lille, CIIL—OpInfIELD, University Lille, F-59000 Lille, FranceLaboratoire de Virologie ULR3610, University Lille and CHU Lille, F-59000 Lille, FranceUniv. Lille, CNRS, Centrale Lille, Univ. Polytechnique Hauts-de-France, UMR 8520, IEMN, F-59000 Lille, FranceLaboratoire de Virologie ULR3610, University Lille and CHU Lille, F-59000 Lille, FrancemiRNAs, small non-coding RNAs that regulate gene expression, are involved in various pathological processes, including viral infections. Virus infections may interfere with the miRNA pathway through the inhibition of genes involved in miRNA biogenesis. A reduction in the number and the levels of miRNAs expressed in nasopharyngeal swabs of patients with severe COVID-19 was lately observed by us, pointing towards the potential of miRNAs as possible diagnostic or prognostic biomarkers for predicting outcomes among patients with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The objective of the present study was to investigate whether SARS-CoV-2 infection influences the expression levels of messenger RNAs (mRNAs) of key genes involved in miRNA biogenesis. mRNA levels of <i>AGO2</i>, <i>DICER1</i>, <i>DGCR8</i>, <i>DROSHA</i>, and Exportin-5 (<i>XPO5</i>) were measured by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) in nasopharyngeal swab specimens from patients with COVID-19 and controls, as well as in cells infected with SARS-CoV-2 in vitro. Our data showed that the mRNA expression levels of <i>AGO2</i>, <i>DICER1</i>, <i>DGCR8</i>, <i>DROSHA</i>, and <i>XPO5</i> were not significantly different in patients with severe COVID-19 when compared to patients with non-severe COVID-19 and controls. Similarly, the mRNA expression of these genes was not affected by SARS-CoV-2 infection in NHBE and Calu-3 cells. However, in Vero E6 cells, <i>AGO2</i>, <i>DICER1</i>, <i>DGCR8</i>, and <i>XPO5</i> mRNA levels were slightly upregulated 24 h after infection with SARS-CoV-2. In conclusion, we did not find evidence for downregulation of mRNA levels of miRNA biogenesis genes during SARS-CoV-2 infection, neither ex vivo nor in vitro.https://www.mdpi.com/1999-4915/15/5/1177miRNASARS-CoV-2RNA interferenceCOVID-19Ago2Dicer |
| spellingShingle | Nathalie Garnier Famara Sane Layal Massara Fabrice Soncin Philippe Gosset Didier Hober Sabine Szunerits Ilka Engelmann Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection Viruses miRNA SARS-CoV-2 RNA interference COVID-19 Ago2 Dicer |
| title | Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection |
| title_full | Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection |
| title_fullStr | Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection |
| title_full_unstemmed | Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection |
| title_short | Genes Involved in miRNA Biogenesis Are Not Downregulated in SARS-CoV-2 Infection |
| title_sort | genes involved in mirna biogenesis are not downregulated in sars cov 2 infection |
| topic | miRNA SARS-CoV-2 RNA interference COVID-19 Ago2 Dicer |
| url | https://www.mdpi.com/1999-4915/15/5/1177 |
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