Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages
Although the neonatal and fetal pathogen Group B Streptococcus (GBS) asymptomatically colonizes the vaginal tract of ∼30% of pregnant women, only a fraction of their offspring develops invasive disease. We and others have postulated that these dimorphic clinical phenotypes are driven by strain varia...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.770499/full |
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| author | Cole R. McCutcheon Macy E. Pell Jennifer A. Gaddy Jennifer A. Gaddy Jennifer A. Gaddy David M. Aronoff David M. Aronoff David M. Aronoff Margaret G. Petroff Margaret G. Petroff Shannon D. Manning |
| author_facet | Cole R. McCutcheon Macy E. Pell Jennifer A. Gaddy Jennifer A. Gaddy Jennifer A. Gaddy David M. Aronoff David M. Aronoff David M. Aronoff Margaret G. Petroff Margaret G. Petroff Shannon D. Manning |
| author_sort | Cole R. McCutcheon |
| collection | DOAJ |
| description | Although the neonatal and fetal pathogen Group B Streptococcus (GBS) asymptomatically colonizes the vaginal tract of ∼30% of pregnant women, only a fraction of their offspring develops invasive disease. We and others have postulated that these dimorphic clinical phenotypes are driven by strain variability; however, the bacterial factors that promote these divergent clinical phenotypes remain unclear. It was previously shown that GBS produces membrane vesicles (MVs) that contain active virulence factors capable of inducing adverse pregnancy outcomes. Because the relationship between strain variation and vesicle composition or production is unknown, we sought to quantify MV production and examine the protein composition, using label-free proteomics on MVs produced by diverse clinical GBS strains representing three phylogenetically distinct lineages. We found that MV production varied across strains, with certain strains displaying nearly twofold increases in production relative to others. Hierarchical clustering and principal component analysis of the proteomes revealed that MV composition is lineage-dependent but independent of clinical phenotype. Multiple proteins that contribute to virulence or immunomodulation, including hyaluronidase, C5a peptidase, and sialidases, were differentially abundant in MVs, and were partially responsible for this divergence. Together, these data indicate that production and composition of GBS MVs vary in a strain-dependent manner, suggesting that MVs have lineage-specific functions relating to virulence. Such differences may contribute to variation in clinical phenotypes observed among individuals infected with GBS strains representing distinct lineages. |
| first_indexed | 2024-12-20T23:38:44Z |
| format | Article |
| id | doaj.art-526121ad7f7e4963ba344e04654c8181 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T23:38:44Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-526121ad7f7e4963ba344e04654c81812022-12-21T19:23:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.770499770499Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse LineagesCole R. McCutcheon0Macy E. Pell1Jennifer A. Gaddy2Jennifer A. Gaddy3Jennifer A. Gaddy4David M. Aronoff5David M. Aronoff6David M. Aronoff7Margaret G. Petroff8Margaret G. Petroff9Shannon D. Manning10Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, United StatesDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, United StatesDivision of Infectious Disease, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United StatesDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, United StatesTennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, TN, United StatesDivision of Infectious Disease, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United StatesDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, United StatesDepartment of Obstetrics and Gynecology, Vanderbilt University Medical Center, Nashville, TN, United StatesDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, United StatesDepartment of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, MI, United StatesDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI, United StatesAlthough the neonatal and fetal pathogen Group B Streptococcus (GBS) asymptomatically colonizes the vaginal tract of ∼30% of pregnant women, only a fraction of their offspring develops invasive disease. We and others have postulated that these dimorphic clinical phenotypes are driven by strain variability; however, the bacterial factors that promote these divergent clinical phenotypes remain unclear. It was previously shown that GBS produces membrane vesicles (MVs) that contain active virulence factors capable of inducing adverse pregnancy outcomes. Because the relationship between strain variation and vesicle composition or production is unknown, we sought to quantify MV production and examine the protein composition, using label-free proteomics on MVs produced by diverse clinical GBS strains representing three phylogenetically distinct lineages. We found that MV production varied across strains, with certain strains displaying nearly twofold increases in production relative to others. Hierarchical clustering and principal component analysis of the proteomes revealed that MV composition is lineage-dependent but independent of clinical phenotype. Multiple proteins that contribute to virulence or immunomodulation, including hyaluronidase, C5a peptidase, and sialidases, were differentially abundant in MVs, and were partially responsible for this divergence. Together, these data indicate that production and composition of GBS MVs vary in a strain-dependent manner, suggesting that MVs have lineage-specific functions relating to virulence. Such differences may contribute to variation in clinical phenotypes observed among individuals infected with GBS strains representing distinct lineages.https://www.frontiersin.org/articles/10.3389/fmicb.2021.770499/fullStreptococcus agalactiaemembrane vesiclesvirulencepathogenesisproteomicsgroup B Streptococcus |
| spellingShingle | Cole R. McCutcheon Macy E. Pell Jennifer A. Gaddy Jennifer A. Gaddy Jennifer A. Gaddy David M. Aronoff David M. Aronoff David M. Aronoff Margaret G. Petroff Margaret G. Petroff Shannon D. Manning Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages Frontiers in Microbiology Streptococcus agalactiae membrane vesicles virulence pathogenesis proteomics group B Streptococcus |
| title | Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages |
| title_full | Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages |
| title_fullStr | Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages |
| title_full_unstemmed | Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages |
| title_short | Production and Composition of Group B Streptococcal Membrane Vesicles Vary Across Diverse Lineages |
| title_sort | production and composition of group b streptococcal membrane vesicles vary across diverse lineages |
| topic | Streptococcus agalactiae membrane vesicles virulence pathogenesis proteomics group B Streptococcus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.770499/full |
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